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Articles by Abdul Ghafoor
Total Records ( 6 ) for Abdul Ghafoor
  Muhammad Ashraf , Afsari Sharif Qureshi , Abdul Ghafoor and Niaz Ahmad Khan
  Thirteen advance lines and three check varieties viz. , Chakwal-86, Pak-81 and Rawal-87 of wheat were planted at nine locations to estimate genotype x environment interaction. Both the linear and non-linear (pooled deviation) components were highly significant, indicating the presence of both predictable and un-predictable components of "G X E" interaction. The stability parameters for the individual genotype revealed that the genotypes, 89R-35 and 90R-36 showed the regression closer to unity along with low deviation from regression and thus may be stated as stable genotypes.
  Nek Dara Jan , Mohammad lqbal , Abdul Ghafoor , Kashif Waseem and Mohammad Saleem Jillani
  A field trail was conducted to assess the effect of different plant spacing (S1 = 50 cm, S2 = 75 cm and S3 = 100 cm) and NPK doses (T1 = control, T2 = 10454-104, T3 = 12474-124, T4 = 144-94-144 and T5 = 164114-164 kg per hectare) on the growth and yield of bottle gourd. The NPK fertilizer doses has significant effect on days to germination, fruit weight (gm), fruit volume (ml), number of fruits per vine, vine length (cm) and yield per hectare (tones). Increasing NPK fertilizer doses also increased the above mentioned parameters. Maximum yield (20.403 t ha–1) was obtained from 15 (164-114-164). Plant spacing had significant influence on days to germination, fruit weight (gm), fruit volume (ml,) number of fruits per vine, vine length (cm) and yield per hectare. Increasing plant spacing increased all the above mentioned parameters, except yield per hectare. Maximum yield (19.709 t ha–1) was obtained from S1 (50 cm).
  Noman Syed , Muhammad Munir , Atiq Ahmad Alizal and Abdul Ghafoor
  Experiment was conducted to study the effect of FYM only and different combinations of nitrogen and potassium on the onion bulbs (var. Swat-I) production. Phosphorous at 100 kg ha–1 was used as a constant dose. Treatments included control, FYM only, 0-100-90, 60-100-90, 90-100-90, 120-100-90, 90-100-0, 90-100-60 and 90100-120 kg NPK ha–1. Control plots and those treated with FYM produced maximum number of culls (un-marketable bulbs) i.e. 29,25 and 27.75 per plot, respectively. Application of fertilizers reduced the number of culls per plot. No significant differences were found among the treatments for bulbs obtained with thick neck. However, increasing rate of N resulted in greater number of thick neck bulbs. Number of double bulbs counted were also increased with the increase of nitrogen. High nitrogen dose improved the bulb size significantly. Greater number of small-sized bulbs were produced from the plots received no fertilizer or FYM only. Significantly highest yield (14.04 t ha–1) was obtained from the plots treated with maximum amount of N (120-100-90). All treatments did not show any significant effect on the percent moisture content, dry weight and TSS.
  Muhammad Ashraf , Afsari S. Qureshi and Abdul Ghafoor
  Ten genotypes of wheat used characterised with their total DNA variation with special reference to known primers of the genes induced in dehydration and salinity stress. Amplification profiles of DRE primers in identification of polymorphic bands that can be used for finger printing the varieties. Four main groups were observed based on 138 polymorphic bands and variance was related to drought, pedigree or geographic origin. Similar pattern of genetic diversity was also observed when 200 markers were recorded from DRE combinations. Pakistani varieties showed higher degree of 70% dissimilarities, While exotic showed low genetic distance.
  Zahoor Ahmad , Abdul Ghafoor and Amanat Ali
  Tropical fodder legumes not only provide superior fodder but also increase the soil fertility due to nitrogen fixing bacteria. For production of fodder during summer, three exotic fodder species, Lablab purpureus, Vigna umbellata and Macroptilium lathyriodes imported from Plant Introduction Station of U.S.A. were evaluated for quality and yield. These crop species were observed carefully and described morphologically. Herbage yield was more than cowpea and significant among all the species, hence could be used as forage crop. Crude protein, ether extract and crude fibre were higher in Macroptilium, whereas herbage yield was significantly higher in lablab bean. Correlation coefficients were different for various crop species which revealed that improvement might be possible through collection and selection for desirable traits, especially in lablab bean which exhibited high correlation between herbage yield and quality characters.
  Muhammad Sajjad IQBAL , Shahid NADEEM , Shahid MEHBOOB , Abdul GHAFOOR , Muhammad Ibrahim RAJOKA , Afsari Sharif QURESHI and Bushra NIAZ
  Nigella sativa L. has industrial, cosmetic, and pharmaceutical uses but has not been adequately characterized in Pakistan. This investigation was carried out to explore genotype specific fingerprinting of 32 N. sativa L. genotypes based on randomly amplified polymorphic DNA markers. From 58 random primers used, 15 primers generated 249 reproducible and scorable amplification products across all the genotypes, out of which 164 (66%) fragments were polymorphic revealing a high level of polymorphism among these genotypes. The proportion of common bands was low (34%). The size of the amplification products on agarose gels ranged between 0.5 and 10.0 kb. In 13 genotypes, 27 bands of different masses (kilobases) were recorded and were considered specific to those genotypes. These specific/amplified PCR products can be used as molecular markers for identification of germplasm and resource protection of Nigella sativa L. genotypes. Specific bands were observed for individual primers that could resolve genetic diversity among several genotypes (PK-020545, PK-020567, PK-020576, PK-020585, PK-020592, PK-020620, PK-020631, PK-020646, PK-020663, PK-020729, PK-020742, PK-020749, and PK-020868). UPGMA cluster analysis indicated 7 distinct clusters, 1 (C-3) comprising 9 accessions of N. sativa L., while C-7, C-5, and C-6 included 7, 6, and 5 accessions, respectively. C-4 and C-2 included 2 accessions each. Cluster 1 remained distinct as it had only 1 accession (PK-020646), indicating its higher genetic diversity from all other species. The overall grouping pattern of clusters corresponded well with principal component analysis and confirmed overall patterns of genetic variability among the species. These genotypes could be used as parents for random mutagenesis, or incorporated for gene recombination studies before marker assisted selection/breeding can be used for crop improvement. Moreover, these DNA based markers can be suitable for genetic distance estimation because they detected potentially large number of polymorphisms. Future research is needed to identify molecular markers linked to important traits (yield and oil quality) and locating quantitative traits loci (QTL) for improvement of N. sativa L. germplasm.
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