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Articles by Abdul Hameed
Total Records ( 6 ) for Abdul Hameed
  Ali Abbas Qazilbash , Rani Faryal , Kokab Batool Naqvi , Safia Ahmad and Abdul Hameed
  This study was designed to ascertain the effectiveness of isolated indigenous Bacillus species to remove chromium from industrially polluted effluents, through a series of effluent biotreatment regimen. Using microbiological techniques, 6 Bacillus strains were isolated, characterized and labeled QIP 1-6. All strains displayed maximum growth on media with a Cr concentration of 400 ppm. Strains QIP 1 and 5 showed maximum resistance to chromium showing moderate growth at 900 ppm of Cr. Following optimization, strain QIP 1 removed 46.81% of the Cr6+ and QIP 5 removed 42.50% of the chromium at the same concentration over the same incubation period, as determined through atomic absorption spectroscopy. Following different biotreatment regimens, biotreatment C1-filtered effluent inoculated with strain QIP 1, incubated under optimum conditions, showed maximum Cr6+ removal (10.29%) after 48 h. Interestingly, the second highest removal of Cr6+ (8.94%) occurred in the untreated, unfiltered raw effluent (D) after incubation for 48 h. Significant (p<0.05) strong positive correlation patterns emerged between these two regimen, as well as between regimen B 1-filtered effluent inoculated with strain QIP 1, but incubated under un-optimized conditions-and regimen D. The study revealed that intrinsic bioremediation does occur naturally and that it is quite possible for a consortium of Bacillus species to work more effectively at removing the HM from the contaminated sites, than a single isolate, thereby reducing the labor intensive work involved.
  Abdul Haleem Shah , Abdul Hameed , Safia Ahmad and Gul Majid Khan
  The objective of this study was to improve the L-lysine production by Corynebacterium glutamicum. For this purpose culture conditions for the lysine fermentation by the bacteria were optimized. In this regard the role of various physical and nutritional parameters was examined. The culture was incubated in a 500 ml Erlenmeyer flask in a rotatory shaking incubator at 200 rpm. The appropriate conditions were obtained only when 50 ml medium was charged for fermentation at 30 oC, pH 7.5 and at 10% inoculum size. The finally selected medium per 100 ml distilled water formulated was 10 g glucose, 2.5 g ammonium sulfate, 2.0 g calcium carbonate, 0.5 g bactocasamino acid, 20 μg thiamine hydrochlorid, 5 μg D-biotin, 0.1 g potassium dihydrogen phosphate, 0.05 g magnesium sulfate heptahydrate, 0.2 mg ferrous sulfate heptahydrate and 0.2 mg manganese chloride tetrahydrate. The optimized culture conditions resulted into elevated amount of L-lysine.
  Shamim Iftikhar , Fauqia Fahmeed , Iftikhar Ahmad and Abdul Hameed
  Powdery scab of potato is caused by Spongospora subterranea f. sp. subterranea. Current study differentiated the powdery scab from deep-pitted scab, which is closely related to common scab (Streptomyces scabies). Pathogen was characterized by light and electron microscopy. Light microscopy (LM) of spores of raised pustules and galls showed a characteristic spongy and honeycomb-like structure. Whereas, spore like bodies of deep-pitted lesions were rounded to oval compact and dark colored bodies. Scanning electron microscopy (SEM) of spore balls of local isolate of S. subterranea revealed no difference in structure on comparison with spore balls of European isolate. Whereas, spore like bodies of deep-pitted scab showed difference in structure. Bioassay and ELISA tests did not show any correlation of spores of powdery and deep-pitted scab.
  Abdul Haleem Shah , Abdul Hameed and Gul Majid Khan
  Lysine is an essential, economically important amino acid used as food and feed supplement. It has also some pharmaceutical applications in the formulation of diets with balanced amino acid composition and in amino acid infusions. Chemical, enzymatic and fermentation processes have been used to synthesize lysine. This review outlines the efforts of various researchers, which provide useful information regarding the fermentative production of lysine by bacteria. It also discusses different methods, including the development of new auxotrophic mutants and optimization of culture conditions used in order to improve the total yield and quality of lysine.
  Agha Asad Noor and Abdul Hameed
  Two strains KA-1 & AAN-2 of Saccharomyces cerevisiae were used as a tool for fermentation and the studies for optimization of different parameters for ethanol yield from corn steep liquor were undertaken. The maximum yield was obtained after 72 hr. at 30 C, 120 rpm in shake flask fermentation. Ethanol production by shake flask fermentation revealed that strain AAN-2 of S. cerevisiae had better results as compared to KA-1 which would be a good candidate of ethanol fermentation on industrial scale.
  Khalid Farooq , Abdul Hameed , Tariq Javed , Ikram Ullah , Abdul Wali Khan and Hassan Khan
  Avian Influenza (AI) has been recognized as a highly contagious and lethal generalized viral disease of birds. In this study, immune response of layers to the commercial oil based and liposomal vaccines of avian influenza H7 was evaluated. Thirty commercial layers were divided into three groups, T1, T2 and T3 with 10 birds in each group. Group T1 served as control, Group T2 was immunized with conventional AI oil-based vaccines, 0.5 mL/bird and Group T3 was immunized with AI Liposomal vaccines 0.5 mL/bird through sub/cut injection. Blood samples were taken and sera were separated at day 0, 7, 14, 21, 28 and day 35. Each time at least 6 samples were taken for antibody titration through AGPT. The geometric mean titre (GMT) of birds in T1, T2 and T3 was 4 ±1.02 at day 0. No significant difference was observed in the titres at day 0 in all the groups. The GMT (Geometric Mean Titer) of control group was 4 ±1.02 at day 7, 6.79 ±1.02 at day 14 and 8 ±1.02 from day 21 to 35. The antibody titre increased slowly from 32 on day 7 to 630.3 on day 35 in birds vaccinated with oil based vaccine, whereas a somewhat quick increase in immune response from 64 on day 7 to 891.4 on day 35 was observed in birds vaccinated with liposomal vaccine. The results showed that immune response of layers in term of GMT was well established with AI liposomal vaccine as compared to that of oil based vaccine. The present study will be helpful in preventing the commercial losses of the farmers and preventing the flock mortality due to the high efficacy of liposomal vaccine against avian influenza.
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