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Articles by Abdoljalal Marjani
Total Records ( 20 ) for Abdoljalal Marjani
  Abdoljalal Marjani
  Information on the measured concentration of serum analytes during storage of serum samples is often incomplete and sometimes contradictory. The 10 analytes have not studied in this area in healthy subjects. The aim of present study was designed to determine the effect of storage time and temperature on the laboratory results of 10 analytes in sera from apparently healthy adult males in city of Gorgan.We studied the effect of storage temperature and time on the measured concentration of 10 serum analytes (2006). Serum was separated from the clot within 20 min of the collection. The sera were stored at 4±1°C and 23±1°C for 0,1, 2, 3, 4, 5, 6, 7, 8, 24, 48 and 72 h, then assayed. Glucose, Phosphorus and creatinine were the least stable and the serum should be determined within 48 h at 4±1°C and 24 h at 23±1°C for these analytes. The other analytes were stable for 72 h. Proper storage temperatures and times must be considered for these analytes (glucose, phosphorus and Creatinine) if measurement is not to take place immediately after specimen collection. Beyond this, it is even very useful to check the reliability of technical and instrumental resources that the laboratory will use during the study because molecular alterations of the analytes due to variable storage conditions can cause misleading results.
  Abdoljalal Marjani and Mohammad Javad Kabir
  Prostate cancer is the most common cancer in men and therefore represents a major problem in public health.The aim of this study was to find and evaluate province-specific estimate of incidence in males by age groups for prostate cancer in Golestan province, Iran. The data used in this study were collected in a cancer registry program that was conducted by Health Deputy of Golestan province in IRAN for a period of 1 year (2004). Prostate cancer data was identified and collected in the population based cancer registries through the 18 Pathology Laboratories (where male populations referred to these centers) and using a structured questionnaire, trained personnel conducted in-person interviews to collect information on prostate cancer in Golestan province. Prostate cancer incidence among males in Golestan province was 5.17/100000 in gerenal. But the highest rate (ASR: 215.87/100,000) among males were showen to be in age 80-85. The incidence of prostate cancer in age 80-84 has risen sharply and it was the lowest in age 50-54 (ASR: 5.18/100,000). According to this information Golestan province harbor a rather incidence for prostate cancer (in age 80-84), comparable to the lower incidence rate reported in the world. For the present time it can be said that prostate cancer in males appear to be one of the most prevalent and serious type of cancer in Golestan province.
  Abdoljalal Marjani , Abdoljabbar Nazari and Mostefa Seyyed
 

Problem statement: In this study, removal of iron was surveyed in well water in Gonbad Kavoos City.
Approach:
Samples were taken before and after aeration. Concentration of iron in each sample was measured by spectrophotometer.
Results:
The results showed that the iron concentration was decreased after aeration (0.55, 0.44, 0.44, 0.46, 0.40 and 0.41 mg L-1) when compared with before aeration of water (0.64, 0.68, 0.65, 0.62, 0.65 and 0.67 mg L-1), respectively.
Conclusion: It was concluded that using aeration technology can reduce iron concentration conform to internationally approved guidelines for iron.

  Abdoljalal Marjani , Hamidreza Joshaghani and Golamreza vaghari
  The aim of this study with the discriminative information was to evaluate the effect of haemodialysis on plasma lipid peroxidation and Cardiac Troponin I before and after the dialysis process. Twenty two patients with Chronic Renal Failure (CRF) disease who were haemodialyzed at 5th Azar hospital of Gorgan Dialysis Center were recruited for this study (2005). Plasma malondialdehyde was increased significantly in the postdialysis group when compared with predialysis. Plasma levels of Cardiac Troponin I in 12 haemodialyzed patients were significantly increased in the postdialysis group when compared with predialysis, whereas plasma level of Cardiac troponin I in 10 haemodialyzed patients showed less than 0.1 μg L-1 which is in normal range. The observation of meaningful increasing level of plasma lipid peroxidation and Cardiac Troponin I in the haemodialyzed patients after the process of dialysis, maybe related with the patient uremia, dialysis membrane and the dialysis process (may increase lipid peroxidation during the dialysis process). These states of affairs may play an important role in progress of cardiovascular abnormality in haemodialyzed patients. Due to this conditions a review of haemodialysis membrane, the techniques used in the dialysis, the consumption of various oral antioxidant, the elimination of active oxygens from the dialysis surrounding are among the measures which can prevent sudden cardiovascular abnormality in the haemodialysis patients and ultimately these important factors up- grade the patients quality of life and prevent sudden silent myocardial infarction.
  Abdoljalal Marjani , Abolvahab Moradi and Araz Berdie Ghourcaie
  The present study was designed to determine the alteration of plasma lipid peroxidation and erythrocyte Superoxide Dismutase and Glutathione Peroxidase enzyme activities in stored blood. This study is carried out to find out the quantitative alterations and the useful length of stored blood. The whole blood were taken from 10 donors. Red Blood Cells (RBC) were counted in whole blood. The levels of Potassium (P) and lactate dehydrogenate activity (LDH) were measured in plasma for determination of hemolysis. The plasma levels of malondialdehyde (MDA) and erythrocyte Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) were studied for determination of lipid peroxidation and antioxidant enzyme activities, respectively. The measurement were performed at the day 0, 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 and 35 of the storage. The plasma levels of malondialdehyde (MDA) and Potassium and lactate dehydrogenate activity increased (p<0.05) depending on storage time whereas erythrocyte Superoxide Dismutase and Glutathione Peroxidase enzyme activities and Red Blood Cells decreased (p<0.05). The alterations of MDA, SOD, GPx, P, LDH and RBC in the measurement days were as follows: MDA, P and LDH significantly increased at the day 9, 5 and 5 whereas SOD, GPx and RBC decreased at the day 11, 7 and 29, respectively. These results suggest that increased level of MDA and decreased level of SOD and GPx in the stored blood can not improve the vialibilty and longevity of RBC by increasing cell damage caused by free radicals at the days 7-11. To improve the quality of stored blood is to supplement blood donors with antioxidants and vitamins at least one week before blood collection. We conclude that increased level of MDA and decreased SOD and GPx in stored blood which can cause the beginning of hemolysis. It is therefore necessary to control these factors before blood transfusion.
  Hamid Reza Joshaghani , Ahmad Ali Shirafkan and Abdoljalal Marjani
  Homocysteine is a sulfur-containing amino acid produced by demethylation of methionine. Genetic mutations and deficiency in nutrients may disrupt homocysteine metabolism and cause an increase in plasma homocysteine level. Approximately 10% of the patients with were cardiovascular disease has elevated plasma homocysteine. Blood sample was drawn from 48 patients who have AMI that without a history of using folic acid or vitamin B complex supply and 48 health people without history of MI, diabetes, hyperlipidemia and hypertension. Homocysteine assayed by Elisa method, folic acid and vitamin B12 performed by RIA method. Mean of homocysteine in case group 30.3±5.3 μM L-1 and in control group 11.1±3.1 (p<0.001). Mean of serum B12 in patients group 297.1±208.9 pM L-1 and in control group 261.5±205.3 that not seen significant difference between two groups. Serum folic acid in patients group was 3.9±2.9 ng mL-1 and in control group was 4.3±3.5 that not significant different in two groups. In this study, level of homocysteine in patients is significantly more than control group. Therefore in this area concentration of homocysteine is very effective in incidence of MI.
  Abdoljalal Marjani , Fatemeh Aliakbari and Mohammad Zaman Kamkar
  The aim of this study was to evaluate the body mass index dependent metabolic syndrome in severe mental illness patients in Gorgan. A total of 267 severe mental illness patients took part in this study. The prevalence of metabolic syndrome in severe mental illness patients in different body mass index were 6.67, 24.09 and 53.06%. There were significant differences in the mean of waist circumference, HDL-cholesterol, triglyceride and fasting blood glucose in subjects with metabolic syndrome in different body mass index when compared with subjects without metabolic syndrome (p<0.05). The prevalence of high fasting glucose, low high density lipoprotein-cholesterol, high triglyceride levels, high waist circumference and high blood pressure were 14.23, 38.57, 41.57, 32.96 and 5.24%, respectively. It shows that high triglyceride levels (41.57%) and Low HDL-cholesterol levels (38.57%) were the most frequent characteristics in comparison to other metabolic components. Our results show that, 26.96, 31.08, 21.35, 15.35 and 5.25% of subjects had zero, one, two, three and four criteria for metabolic syndrome, respectively. These results show that the prevalence of metabolic syndrome in severe mental illness patients in Gorgan is increased with elevated body mass index. The results of this study suggest that mental illness patients are at risk of metabolic syndrome, when the rate of body mass index increases. Risk factors such as high triglyceride level and low HDL-cholesterol may play an important role in the occurrence of metabolic syndrome in severe mental illness patients.
  Mojdeh Ghiyas Tabari , Fatemeh Naseri , Edris Paad , Fatemeh Majidi and Abdoljalal Marjani
  Epidemiological studies have shown the importance of the metabolic syndrome. With estimation of the metabolic syndrome, it may predict cardiovascular disease, sudden death and the presence of some other cardiovascular risk factors. The aim of this study was to assess the metabolic syndrome among Baluch women. Our study consisted of 120 Baluch women. Baseline data of Baluch women, prevalence of metabolic syndrome and its components and distribution of body mass index were determined. The mean Body Mass Index (BMI), waist circumference, systolic blood pressure, triglyceride, High Density Lipoprotein-Cholesterol (HDL-Chol.) and fasting blood glucose levels were significantly higher in the subjects with metabolic syndrome. The prevalence of HDL-cholesterol, high triglyceride, high fasting glucose levels, high waist circumference and high blood pressure were shown to be 33.3, 20.8, 12.5, 11.8 and 2.5%, respectively. HDL-cholesterol (33.3%) and high triglyceride levels (20.8%) were the most frequent characteristics of metabolic components. The prevalence of subject with normal weight, overweight and obese BMIs were 77.5, 15 and 7.5%, respectively. About 9.17, 4.17 and 4.17% of Baluch women had three, four and five criteria of metabolic syndrome components, respectively. This study reveals that there is a significant difference in the metabolic syndrome components in patients with and without metabolic syndrome. The prevalence of HDL-cholesterol and high triglyceride in Baluch women was highest. Definition of metabolic syndrome may help physicians to estimate, decrease and prevent coronary heart disease and cardiovascular morbidity and mortality in subjects with metabolic syndrome.
  Mohammad Jafar Golalipour , Anneh Mohammad Gharravi , Abbas Ali Keshtkar and Abdoljalal Marjani
  This study was done to determine the effects of subacute exposure of Peracetic acid on Hematological indices in Wistar rats. In this study 18 male Wistar rats divided to two experimental and one control groups. PAA with 99% purity purchased. Then 0.2 and 2 mL of PAA dissolved in 100 mL drinking water. Animals in Treatment Group 1 and 2 received 0.2% PAA daily for 4 weeks and 2% PAA daily for 4 weeks, respectively. After the animals had been sacrificed hematological parameters examined. Experimental results concerning this study were evaluated using SPSS v.11.5 and expressed as Mean ±SD p<0.05 was considered significant. Mean ±SD of WBC of groups 1 (2.45 ±0.77) and 2 (3.63 ±0.23) decreased significantly as compared with control group (5.3 ±0.57). RBC in control and 1, 2 groups were 8.34 ±0.39, 7.48 ±0.11 and 7.61 ±0.46, respectively. Also HCT and PLT decreased significantly in groups 1 and 2 as compared with control group. This study showed that oral consumption of Peracetic acid with concentration 0.2, 2% for 4 weeks can cause decrease hematological parameter in animal model.
  Abdoljalal Marjani , Azad Reza Mansourian , Gholam Reza Veghari and Mohammad Reza Rabiee
  Free radicals have been proposed as important causative agents of ageing. The free radical theory of ageing postulates that ageing is caused by free radical reactions. These highly reactive species can cause oxidative damage in the cell. The purposive of this study was to investigate the alteration in plasma lipid peroxidation and erythrocyte superoxide dismutase activity in 2 different ethnic groups of Fars and Turkmen healthy people. We measured plasma lipid peroxidation levels (lipid peroxidation expressed as malondialdehyde) and erythrocyte superoxide dismutase activity. Study include 350 (175 Fars and 175 Turkmen male) apparently healthy individuals. Erythrocyte superoxide dismutase activities were determined in 2 different ethnic groups of Fars and Turkmen consisting of healthy individuals between 26-60 years of age {26-30 (n = 30), 3-35 (n = 30), 36-40 (n = 30), 41-45 (n = 30), 46-50 (n = 25), 51-55 (n = 15) and 56-60 (n = 15)}, respectively. The data was analyzed by Student` t-test. Erythrocyte superoxide dismutase and plasma lipid peroxidation levels in Fars and Turkmen people with 41-45 ages (group 4) and 36-40 ages (group 3) were significantly lower and higher than in the other age groups (Fars groups 1, 2 and 3, Turkmen groups 1, 2), respectively (p< 0.05). There were no significant relation between the age group 4 (Fars people) and the age groups 5, 6 and 7 (p>0.05). There were no significant relation between the age groups 3 (Turkmen people) and the age groups 4, 5, 6 and 7 (p>0.05). We found age-related differences in erythrocyte superoxide dismutase activity and plasma lipid peroxidation levels. The results indicate that the balance between antioxidant and prooxidant factors in free radical metabolism shifts towards increased lipid peroxidation with advancing age in 2 ethnic groups. This situation maybe begin in Turkmen people earlier than Fars people. The ethnic origin, diet, heavy working and life style factors of the two populations may explain this differences. Therefore we propose that older Fars and Turkmen people may have elevated requirement for antioxidants. Supplementation with vitamin or dietary free radical scavengers such as vitamin E and C or foodstuff containing these such as tomatoes, oranges and similars have a potential role in boosting antioxidant related defenses and may be important for older people of two ethnic groups.
  Abdoljalal Marjani
  The metabolic syndrome increases the risk of many diseases. There are many investigations on the role and alterations of High-Density Lipoprotein (HDL)-cholesterol in different diseases. HDL-cholesterol is an important component of metabolic syndrome. Low HDL-cholesterol has been shown among subjects with metabolic syndrome. Low levels of HDL-cholesterol are collaborated with elevated risk of cardiovascular disease, coronary heart disease, myocardial infarction, stoke and Alzheimer disease. The increased prevalence of metabolic syndrome and its relation with low levels of HDL-cholesterol accentuate its diagnostic importance and medical care. The aim of present study was to review HDL-cholesterol alterations in metabolic syndrome.
  Abdoljalal Marjani
  The metabolic syndrome significantly elevates the risk of cardiovascular disease and renal damage. Because of the relationships among different components of metabolic syndrome, it is difficult to understand the cause of these diseases. Blood pressure is one of the most factors of cardiovascular risk and disease. Hypertension is in many cases underrated and undiagnosed. It is suggested that there are different mechanisms to play a role in the blood pressure elevation in different subjects. Elevated blood pressure is believed a significant component of metabolic syndrome. Individuals with metabolic syndrome have been shown increased blood pressure or hypertension. It is reported that metabolic syndrome expand hypertension related cardiac and renal damage. The treatment of subjects with the metabolic syndrome is important to decrease the risk of cardiovascular and renal diseases, diabetes and hypertension and organ damage in the future. In present study, it was reviewed with a number of related articles on the role of hypertension (as one of the metabolic syndrome components) in metabolic syndrome.
  Abdoljalal Marjani
  In this study the plasma level of zinc and Magnesium were determined in 50 type 2 diabetic patients and 50 healthy people. The sampling of type 2 diabetic patients were randomized. Diabetic patients studied were without any complications. Samples were analyzed using Randox kit with Spectrophotometric method. The plasma zinc (116.78±5.51 μg dL-1) and Magnesium (1.55±0.12 meq L-1) levels significantly decreased in type 2 diabetic patients (p<0.001) when compared with control groups (146.86±9.06 μg dL-1, 1.84±0.10 meq L-1, respectively). It is concluded that type 2 diabetes mellitus can result in changes in Zinc and Magnesium levels. The decreased plasma Zn and Mg that we demonstrated in this study in patients with type 2 diabetes mellitus probably reduce insulin sensitivity and may increase risk of secondary complications, it maybe prudent in clinical practice to periodically monitor plasma Zn and Mg concentrations in type 2 diabetic patients. If plasma Zn and Mg were decreased, an intervention to increase of dietary intake of Zn and Mg may be beneficial for these patients.
  Abdoljalal Marjani , Mohammad Javad Kabir , Shahriyar Semnani , Seyyed Mehdi Sedaghat and Abbas Moghaddami
  The main aim of this study was to find and describe province-specific estimates of incidence in females by age groups for esophagus cancer. The data used in this study were collected in a cancer registry that was conducted by Health Deputy of Golestan province for a period of 1 year (2004). The age distribution was collected according to the following age strata: 0-4, 5-9, 10-14, 15-19, 20-24, 25-29, 30-34, 35-39, 40-44, 45-49, 50-54, 55-59, 60-64, 65-69, 70-74, 75-79, 80-84 and 85 above. Esophagus cancer data was identified and collected through the 18 Pathology Laboratory centers (where female populations referred to these centers) in Golestan province. A total of 348 primary cancer cases were captured. From these 32 cases was esophagus cancer. There were 26 squomous cell carcinoma (81.25%), 1 adenocarcinoma (3.12%). Esophagus cancer incidence among females in Golestan province was 7.62/100000. But esophagus cancer with the highest ASR: 127.91/100,000 was in age 75-79. The incidence of esophagus cancer in age over 75-79 has risen sharply and it was the lowest in age 30-34 (ASR: 1.68/100,000). For the present time it can be said that esophagus cancer in females appear to be one of the most prevalent and serious type of cancer (especially squomous cell carcinoma) in Golestan province and esophagus cancer is rising with ageing.
  Abdoljalal Marjani , Abdolvahab Moradi and Mohsen Saeedi
  The aim of this study was to determine the changes of plasma lipid peroxidation, zinc and erythrocyte Cu-Zn superoxide dismutase activity in patients with type 2 diabetes mellitus and healthy control in Gorgan city. Fifty type 2 patients with diabetes mellitus and 50 people without diabetes were included in this randomized study. None of patients studied had any diabetic complications. The levels of plasma malondialdehyde and zinc from type 2 diabetes mellitus patients (6.24±0.85 nmol mL-1 and 116.78±5.51 μg dL-1) and control groups (3.63±0.97 nmol mL-1 and 146.86±9.06 μg dL-1) were determined. Erythrocyte Cu-Zn superoxide dismutase activity from patients with diabetes (675.34±60.89 U g-1 Hb) and control groups (1052.70± 52.76 U g-1 Hb) were determined. The increased plasma lipid peroxidation and decreased plasma zinc and erythrocyte Cu-Zn superoxide dismutase activity that we observed in patients with type 2 diabetes mellitus may predispose to the development of cardiovascular complications. We propose that diabetic patients may have elevated requirement for antioxidants. Supplementation with zinc and vitamin or dietary free radical scavengers such as vitamins E and C or tomato, orange and etc. have a potential role in boosting antioxidant-related defences and maybe important in patients with diabetes.
  Arash Rafeeinia , Hanieh Teymoori and Abdoljalal Marjani
  Etiology of preeclampsia is clearly unknown. The aim of this study was to assess thyroid hormone levels in mild and severe preeclampsia women and compare them with healthy pregnant women. This study was done on 50 healthy pregnant and 50 preeclampsia women in the third trimester in the Sayyad Shirazi educational Hospital, Gynecology Department of Golestan University of Medical Sciences, Iran, 2014. There were significant differences in T4 level among mild preeclampsia and healthy pregnant women. There were also significant differences in T4 and T3 levels between severe, mild preeclampsia women and healthy pregnant women. We observed a significant positive correlation between systolic and diastolic blood pressure and thyroid hormones. Serum T3 and T4 levels were significantly increased with development of preeclampsia. There is association between thyroid hormone abnormalities and hypertension. The difference of our study with other findings could be related to different geographical areas, races and diets. Variation of thyroid functioning later in life may develop in preeclampsia women. Thus, it suggests that thyroid function test may necessary to screen preeclampsia women during pregnancy and after parturition.
  Azam Shafaei , Masoud Khoshnia and Abdoljalal Marjani
  Effect of FGF21 on metabolic syndrome patients is not exactly clear. In the present study, we assessed serum level of fibroblast growth factor 21 in type 2 diabetic patients with and without metabolic syndrome in Gorgan. The study groups consisted of 120 patients with type 2 diabetes and 60 healthy subjects. Diabetic patients divided into two groups. All subjects were matched according to age and sex. The mean waist circumference, systolic and diastolic blood pressure, triglyceride and fasting blood glucose levels and body mass index were significantly higher in the subjects with metabolic syndrome than control group, but the mean HDL-cholesterol was significantly lower (p = 0.0001). Mean serum level of FGF21 was higher in type 2 diabetic subjects with and without metabolic syndrome than that of control subject (p = 0.0001). There were significant negative correlation between FGF21 and BMI, diastolic blood pressure and HDL-cholesterol in subjects with metabolic syndrome (p<0.05). There were also significant negative correlation between FGF21 and cholesterol and HDL-cholesterol in control group (p<0.05). The findings of this study suggest that serum FGF21 are higher in patients with type 2 diabetes with and without metabolic syndrome than in age and sex matched control group. Our study shows that some of metabolic syndrome components (especially HDL-cholesterol) are associated with high serum FGF21 levels. Because of different effects of FGF21 in rodents, primates and in humans, it may require more investigating on metabolic effects of FGF21 in human’s samples.
  Tayebe Beigi , Azam Shafaei , Masoud Khoshnia and Abdoljalal Marjani
  Some studies have indicated that increased levels of liver enzymes may associate with development of diabetes in future. The association between liver enzymes and type 2 diabetes are inconsistent and may depend on the ethnic difference among different populations. The aim of present study was to assess serum Fetuin A level, liver enzymes activities and insulin resistance in patients with type 2 diabetes in Gorgan. The study groups consisted of 75 type 2 diabetic patients and 75 control subjects. Both subjects were matched for age and sex. Different parameters were in the Metabolic Disorders Research Center. There were significant differences in the mean value of glucose, triglyceride, Low Density Lipoprotein (LDL), Alanine Transaminase (ALT), γ-glutamyltransferase (GGT), insulin, HOMA-IR and fetuin A when type 2 diabetic patients compared with control groups. There were significant negative correlation between fetuin A and age in subjects with type 2 diabetic patients and control groups (p<0.05). The present study showed that serum fetuin-A levels are significantly increased in type 2 diabetes mellitus. Our findings show that studied liver enzymes were higher in type 2 diabetic patients than control groups. The relationship of liver aminotransferase levels and risk of type 2 diabetes development seems to be complex. Increased levels of fetuin-A and studied enzymes in subjects should be an alert for further clinical evaluation and screening.
  Mohsen Saeedi , Gholam Reza Veghari and Abdoljalal Marjani
  This study is a cross sectional and descriptive study. Sample population was the women referred for marriage consultation to Gorgan Marriage Consultation Center in 2004. The prevalence of toxoplasma antibodies (IgG, IgM) was determined by ELISA method. Blood samples were collected randomly from 300 women referred to the center for consultation. They were transferred to the laboratory for antibodies determination. Gorgan located in north of Iran and south east of Caspian sea. The results showed that the general prevalence to the positive cases based on high titer of IgG was 48.3% and of IgM was 11.7%. There was not any relation between positive cases and age, education, place of residence, job, keeping domestic animals (except cat) vegetable consumption and wash-up, but there was a relation between the positive cases of IgM and keeping cat at home (PV<0.025). More over 51.7% of pregnant women in Gorgan were seronegative and the were prone to acute toxoplasmosis during their pregnancy. The results of this study confirm that the determination of the diagnostic toxoplasmosis is an necessary test during pregnancy.
  Abdoljalal Marjani
  The aim of the present study was designed to determine the alteration of plasma lipid peroxidation-(by measuring the level of MDA) and total antioxidant status in stored blood. This study is carried out to find out the quantitative alterations and the useful length of stored blood. The whole blood were taken from 10 donors. Red Blood Cells (RBC) were counted in whole blood. The levels of Potassium (P) and lactate dehydrogenate activity(LDH) were measured in plasma for determination of hemolysis. The levels of malondialdehyde (MDA) and Total Antioxidant Status (TAS) were studied in plasma for determination of lipid peroxidation and antioxidant system, respectively. The measurement were performed at the day 0, 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 and 35 of the storage. The levels of malondialdehyde and Potassium and lactate dehydrogenate activity increased (p<0.05) depending on storage time whereas Total Antioxidant Status (TAS) and Red Blood Cells decreased (p<0.05). The alterations of MDA, TAS, P, LDH and RBC in the measurement days were as follows: MDA, P and LDH significantly increased at the day 9, 5 and 5 whereas TAS and RBC decreased at the day 13 and 29, respectively. These results suggest that increased level of MDA and decreased level of TAS in the stored blood can not improve the vialibilty and longevity of RBC by increasing cell damage caused by free radicals at the day mentioned above. To improve the quality of stored blood is to supplement blood donors with antioxidants and vitamins at least one week before blood collection to keep and store the stored blood for longer time until using for transfusion. We conclude that increased level of MDA and decreased TAS in stored blood which can cause the beginning of hemolysis. It is therefore necessary to control these factors before blood transfusion.
 
 
 
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