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Articles by Abdinasir Yusuf Osman
Total Records ( 4 ) for Abdinasir Yusuf Osman
  Faez Firdaus Jesse Abdullah , Abdinasir Yusuf Osman , Lawan Adamu , Zunita Zakaria , Rasedee Abdullah , Mohd Zamri-Saad and Abdul Aziz Saharee
  Acute Phase Protein (APP) investigations of serum or plasma following natural or experimental infection frequently reveal substantial alterations in the APPs, several of which are of veterinary importance in the assessment of herd health. The present study of the experimental nature was conducted to evaluate the acute phase protein responses; haptoglobin, Serum Amyloid A (SAA) and serum albumin in relation to infection with Pasteurella multocida type B and its immunogens; lipopolysaccharide (LPS) and Outer Membrane Protein (OMP) in calves. Eight clinically healthy, non-pregnant and non-lactating Brangus cross calves weighing 150±50 kg were used in this study. The calves (n = 8) were divided into 4 groups of 2 calves in each group. The control group was inoculated with sterile Phosphate Buffered Saline (PBS) whereas group 2 were inoculated with wild-type P. multocida type B:2 and group 3 and 4 were inoculated with LPS and OMP respectively. Blood samples were collected via jugular vein-puncture at 3 h intervals for APPs analysis. APPs were quantified by commercially available ELISA methods. Moribund animals were euthanized while the surviving animals were killed after 48 h. The results revealed that there were statistically significant differences (p<0.05) between APPs concentrations throughout the experimental period in challenged groups compared to control groups. Over 7-fold increase was observed in Hp concentrations with mean maximum levels of 1.316±0.558 ng mL-1, 1.521±0.687 ng mL-1 after experimentally induced with OMP and LPS respectively. SAA increased less than 3-fold with mean levels of 2.187±0.880 ng mL-1, 2.421±0.432 ng mL-1, 2.657±0.099 ng mL-1 in almost all challenged groups; Pasteurella Multocida, OMP and LPS respectively. In contrast, for the negative APP, the albumin levels of groups LPS and OMP did not show significant difference (p>0.05) with mean levels of 32.677±1.556 and 36.185±2.239 U L-1, respectively. While P. multocida group (22.193±2.727 U L-1) showed statistically significant difference (p<0.05) than the negative control group (34.233±6.900 U L-1). In conclusion, the findings of this study indicated that APPs; SAA and haptoglobin are sensitive biomarkers to explore host response in relation to Haemorrhagic Septicaemia infections in clinical settings.
  Abdinasir Yusuf Osman , Faez Firdaus Jesse Bin Abdullah and Abdul Aziz b Saharee
  Seroepidemiological survey of Caseous Lymphadenitis (CLA), a bacterial infection in sheep and goats was conducted in East Coast Economic Regions (ECER) in peninsular Malaysia. A total of 422 animals (n = 422) from 15 small ruminant farms (sheep and goats) in the four states were screened during the period from January to April 2011. The management practices that could be associated with CLA occurrence was also analyzed. The objective of this survey was to determine the overall status of CLA infections in small ruminants. AGPT was conducted on serum samples collected from animals. About 47 samples out of 422 yielded a positive reaction for AGPT test. The frequency of CLA infections was estimated at 11.1%. Management practices were analyzed through questionnaire. All farmers (15/15) had semi-extensive rearing system; 27.6% (13/47) of farmers had periodical veterinary services; 10.6% (5/47) took note of animals with clinical signs of CLA; 14.8% (7/47) were aware of the zoonotic potential of this disease. Lack of sanitary measures and epidemiological studies led the dissemination of this disease in Malaysia. Moreover, the economic importance of this disease seems to be overlooked by both individual and state level allowing existence of endemic nature of the disease in this country. Therefore, further epidemiological studies using reliable diagnostic tools and application of appropriate management practices need to be implemented for aiding control and eradication programs for this disease.
  Abdinasir Yusuf Osman , Faez Firdaus Jesse Abdullah , Abdul Aziz Saharee , Abd Wahid Haron , Jasni Sabri and Rasedee Abdullah
  Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis, a chronic infectious disease of sheep and goats. Despite the growing interest of this organism, little is known about the causality and effect of this organism on haematological and biochemical values. The purpose of this research, therefore was to determine haematological and serum biochemical features in mice inoculated with whole bacterium and exotoxin (PLD) extracted from C. pseudotuberculosis. The study was performed on apparently healthy mice of 2-3 weeks old (n = 64). The mice were divided equally into 3 groups; namely whole bacterium, exotoxin (PLD) and control group. Mice of whole bacterium group were exposed intraperitoneally to 1 mL of the inoculums containing 109 Colony-Forming Unit (CFU)/mL of live C. pseudotuberculosis. Exotoxin group were exposed intraperitoneally with a single dose of exotoxin (PLD) extracted from C. pseudotuberculosis. Mice served as a control group were challenged intraperitoneally to 1 mL of Phosphate-Buffered Saline (PBS), pH 7. Following post-infection, the mice were subjected for blood sample collection using cardiac puncture method for haematological and biochemical analysis. The results of this study revealed that there were significant decrease (p<0.05) in Hb, thrombocytes count and significant increase in WBC, neutrophils, monocytes counts in the infected group. Biochemically, there were highly significant increase (p<0.05) in the mean levels of CK, AST, ALP and ALT. The mean level of albumin in exotoxin group was significantly lower (p<0.05) compared to C. pseudotuberculosis and control groups while globulin level was significantly higher in C. pseuodotuberculosis group after 48 h. In serum electrolytes, mean level of potassium and phosphate were significantly (p<0.05) higher in infected groups compared to control group while there was no significant (p<0.05) difference in the levels of sodium, calcium and chloride. In conclusions, the present study of experimental nature showed that C. pseudotuberculosis and its exotoxin (PLD) cause disturbances in blood factors and electrolytes and pointed out that t hese haematological and biochemical alterations should be taken into account in the context of diagnosis and treatment of valuable infected hosts in order to prevent further consequences.
  Faez Firdaus Jesse Abdullah , Lawan Adamu , Abdinasir Yusuf Osman , Mohd Zamri Saad , Zunita Zakaria , Rasedee Abdullah and Abdul Aziz Saharee
  Haemorrhagic Septicaemia (HS) is a killer disease of cattle and buffalo of economic importance in Asia and Africa. There is insufficient information on the responses of Balb c mice as animal model in respect of immunogens and Acute Phase Proteins (APP) profiles. Therefore, the present study aims to evaluate the acute phase protein profiles in mice associated with the infection of Pasteurella multocida type B and the bacterial lipopolysaccharide and outer membrane protein immunogens. Two hundred healthy Balb/c mice of 8-10 weeks old were used in this study. They were divided into four equal groups of 50 mice each. Mice of group 1 were inoculated intra-peritoneal with 1.0 mL sterile Phosphate Buffered Saline (PBS) pH 7, group 2 were inoculated with 1.0 mL of 109 colony forming unit (cfu) of P. multocida B: 2. Mice of groups 3 and 4 were inoculated intra-peritoneal with 1.0 mL of LPS and 1.0 mL of OMP, respectively. Acute phase proteins analysis were done using two sites Enzyme Linked Immunoassay (ELISA) highly sensitive test kits. The data was analyzed using SPSS. Haptoglobin concentration increased significantly in group 3 and 4 (p<0.05) following inoculation with immunogens compared to control group. Mice in group 3 and 4 showed significantly (p<0.000) 3 times higher concentrations of SAA and significantly (p<0.037) 1.3 times increased concentrations of SAA, respectively compared to the control group. There was no significant changes in the concentrations of fibrinogen in group 2 (p = 0.177), group 3 (p = 0.088) and group 4 (p = 0.359). C-reactive protein in groups 2 and 3 showed significantly (p<0.05) higher levels than the control group. Albumin showed significant increase (p<0.05) in group 2 compared to the control group. There were significant changes in the concentrations of acute phase proteins and clinical responses post inoculation with immunogens indicating adverse pro-inflammatory reactions in mice in the present study.
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