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Articles by AbdelRahim E. Karrar
Total Records ( 3 ) for AbdelRahim E. Karrar
  Mohamed A.M. Yousof , Imadeldin E. Aradaib , Tamadour M. Abdalla , AbdelRahim E. Karrar , Kamal E.E. Ibrahim , Mohamed A. Abdalla and Abdelrahim M. Hussein
  In this study, a nested Reverse Transcriptase (RT) polymerase chain reaction (RT-PCR)-based assay, for detection of Newcastle Disease Virus (NDV) Ribonucleic acid (RNA) in clinical samples from experimentally infected chicks, was evaluated. The clinical samples used included, blood, tracheal, cloacal, liver, spleen, heart, lung, kidney and brain. The nested RT-PCR was performed in two amplification steps. In the first step, a pair of primers (nd and nd ) was used to amplify a 356 bp specific region in the F gene of NDV. In the second step, a nested pair of primers (nd and nd ) was employed to produce 216 bp amplification products, internal to the annealing sites of primers nd and nd . The 356 bp PCR products were amplified only from lung homogenate, cloacal and tracheal tissues, kidney, heart and brain. However, the 216 bp nested amplification was detected in all tissue samples collected from experimentally infected chicks. The nested amplification confirmed the identity of the first amplified product and increased the sensitivity of RT-PCR assay. RNA samples extracted from Infectious Bursal Disease Virus (IBDV) and Infectious Bronchitis virus (IBV) or total nucleic acid extracted from blood of non infected birds failed to demonstrate the primary or the nested PCR products. The described nested RT-PCR assay provide reliable, rapid, sensitive and specific diagnostic assay for detection of an outbreak of NDV infection among susceptible Birds.
  Khairalla M.S. Khairalla , Imadeldin E. Aradaib , Tigani Hassan , Ali A. Majid , Abdelrahim E. Karrar , Ahmed M. A. Osman and Osman A. Osman
  A nested Polymerase Chain Reaction (PCR) assay for specific identification of pork or swine-derived products in processed food and in animal feed concentrates was developed and evaluated. The mitochondrial cytochrome-b (mtcyt-b) gene was used as a target DNA for PCR amplification. Two pairs of primers (PSL1 and PSR2) and (PSL3 and PSR4), were used for the nested PCR in two amplification steps. First the outer pair of primers (PSL1 and PSR2), derived from a highly conserved region of swine mtcyt-b gene, produced a 1055 base pair (bp) PCR amplicon from swine DNA. Amplification products were visualized on ethidium bromide-stained agarose gels from 100 fg of swine DNA equivalent to 1000 copies of mtcyt-b gene. The nested primers (PSL3 and PSR4) produced a 361 bp PCR product, internal to the annealing sites of primers (PSL1 and RSR2). The nested amplification confirmed the identity of the primary amplified PCR product and increased the sensitivity of the PCR assay. The nested PCR with ethidium bromide-stained agarose gels detected the amount of as little as 0.001 fg of DNA (equivalent to a single copy of Swine-mtcyt-b gene). The specificity studies indicated that neither the primary 1055 bp nor the nested 361 bp PCR products were detected from DNA extracted from a variety of other animal species including, sheep, goat, cattle, deer, camel, horse, donkey, chicken and fish. Application of this nested PCR to processed food including, fresh pork, smoked ham, marinated pork, canned luncheon, petís food, poultry feed resulted in amplification of the swine specific PCR products. The described nested PCR provides a valuable tool to authenticate the presence of swine-derived product in processed food and in commercial animal feed concentrates.
  Mohamed E. Ahmed , Imadeldin E. Aradaib , AbdelRahim E. Karrar , Badr E. Hago and Safa A. Sherfi
  Two patients (adult females) were admitted to Ahmed Gasim Medical Hospital, Khartoum North, Sudan, with history of severe combined mitral regurgitation and stenosis. The patients were referred to the thoracic surgery unit for mitral valvectomy and valve replacement. Both patients received early empirical prophylactic antibiotic therapy half an hour before as well as post operatively. However, they developed fever shortly after the operation. Blood samples were collected and submitted to the diagnostic laboratory for conventional and molecular microbiological examinations. Conventional bacteriological examination revealed that blood cultures were negative for any bacterial growth. However, the Polymerase Chain Reaction (PCR) detected a 486 bp PCR product specific for E. coli. The identity of the nucleic acid sequence was confirmed by nested amplification of a 186 bp PCR product from the primary PCR product. The scientific data presented in this study indicated that PCR provides a rapid method for detection of E. coli during bacteraemia, irrespective of their viability. However, conventional bacterial isolation methods failed to diagnose E. coli infection in patients receiving high doses of antibiotics.
 
 
 
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