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Articles by A.V. Raghu
Total Records ( 4 ) for A.V. Raghu
  A.V. Raghu , Gerald Martin , V. Priya , S.P. Geetha and Indira Balachandran
  The study of cost reduction alternatives in micropropagation of Centella asiatica was reported. Low cost options adopted in the study were effective in lowering cost of production without compromising the quality of plants. The cost on media ingredients was reduced by using household sugar instead of laboratory grade sucrose and tap water instead of double distilled water. Use of liquid media during multiplication stage also helped in reducing the cost on agar. Rooting of in vitro developed shoots under ex vitro conditions also helped cost effectiveness.
  A.V. Raghu , S.P. Geetha , Gerald Martin , Indira Balachandran , P.N. Ravindran and K.V. Mohanan
  An efficient and rapid in vitro clonal propagation of the endangered medicinal tree Aegle marmelos (L.) Corr. (Rutaceae) by enhanced axillary shoot proliferation from mature single node was designed. The explants showed marked seasonal variation in their response under in vitro conditions. Explants collected in October (72.8%) and November (78.6%) showed maximum response. Multiple shoots were formed on Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 6-Benzyladenine (BA). An average of 6.2 shoots/explant could be obtained after 45 days of culture. The number of shoots was increased at the third subculture with an average of 16.3 shoots per explant. The effect of subsequent subcultures (upto 20 cycles) on shoot formation was also studied. Subculturing was carried out every 45 days on fresh shoot multiplication medium. Continuous culture in the same medium resulted in distorted and vitrified shoots. Transfer of cultures to half strength MS medium devoid of ammonium ions and cytokinin (BA) for a single cycle before going to the shoot multiplication medium could solve this problem. In vitro rooting was inconsistent in medium with different auxins (Indole 3-butyric acid-IBA, Indole 3-acetic acid-IAA and α-naphthalene acetic acid-NAA) at varying concentration and combinations. But in vitro raised shoots could be rooted ex vitro by pulse treatment with naphthoxy acetic acid (NOA) and IBA and then in chlorogenic acid followed by planting in moist sand. This treatment resulted in 83.9% survival of plantlets. The method standardised could be used for large scale planting material production and conservation of this important endangered medicinal tree.
  A.B. Remashree , T. Varghese Thomas , A.V. Raghu , E. Nabeesa and N. Neelakandan
  An efficient protocol for the in vitro propagation of the valuable medicinal plant, Syzygium cumini L. is described through axillary bud proliferation. Multiple shoots were induced from mature nodal explants cultured on Woody Plant Medium (WPM) supplemented with combinations of Benzyl Adenine (BA; 8.8 μM), Kinetin (9.3 μM) and Naphthalene Acetic Acid (NAA; 5.37 μM) produced an average of 35-40 shoots by 6-7 weeks after inoculation. The shoots were rooted in WPM supplemented with 4.9 μM Indole-3-butryic acid (IBA). Ex vitro rooting was also successful in this species. Plantlets established in the field showed 80% survival and exhibited identical morphological characteristics as the donor plant.
  P.K. Usha , Sailas Benjamin , K.V. Mohanan and A.V. Raghu
  An efficient protocol was established for in vitro shoot multiplication from shoot tip explants of Vitex negundo on Murashige and Skoog (MS) basal medium supplemented with 8.87 μM 6-benzylaminopurine (BA). Inclusion of 8-Naphthalene Acetic Acid (NAA) in the culture medium along with BA promoted higher rates of shoot multiplication than BA alone. The rate shoot multiplication (6.3) after 4 week of culture on MS basal medium supplemented with 8.87 μM BA, 2.69 μM NAA. The elongated shoots rooted in half strength MS basal salts supplemented with 4.90 μM IBA + 2.85 μm IAA and 2% (w/v) sucrose. The presence of Activated Charcoal (AC) with IBA showed positive response to rooting. In vitro propagated plants were transferred to soil with a survival rate of 85% after 1 month.
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