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Articles by A.S. Traore
Total Records ( 10 ) for A.S. Traore
  M.K. Somda , A. Savadogo , C.A.T. Ouattara , A.S. Ouattara and A.S. Traore
  The use of mango peel (agricultural waste with no appreciable value to industries or competitive use as food) as alternative and cost-effective biomass for the production of bioethanol was examined. Thus, the aim of this study was to select performed yeasts strains able to ferment sugars containing in peel mango. The methods used, included: enzymatic hydrolysis with Bacillus and simultaneous saccharification and fermentation with W6 and B1, yeasts isolated from wine and among bakers yeasts, respectively. The kinetics parameters results showed that the optimum alcohol releasing like 35% (g E/g S) and 30% (g E/g S) respectively from W6 and B1 be spread out at 40°C. The maximum volume of ethanol produced from peel mango by W6 and B1 were, respectively 13 and 10.1 g L ¯1 at the 120th.
  M.K. Somda , A. Savadogo , C.A.T. Ouattara , A.S. Ouattara and A.S. Traore
  Saccharomyces is a potentially useful organism for the commercial production of ethanol as it is not capable of fermenting starch containing in the mango peel. To enhance this carbon source utilization and increase the rate of alcohol production, simultaneous saccharification and fermentation were conducted using Bacillus licheniformis and yeasts. The proposal of this research was to study amylasic activity, rate and amount of starch utilization and ethanol yields increasing several-fold in coculture due to the synergistic metabolic interactions between the species. The methods used, included: enzymatic hydrolysis with B. licheniformis and simultaneous saccharification and fermentation with S3 and A1. These two yeasts strains were isolated from wine and among local beer yeasts respectively. The enzymatic hydrolysis has allowed to obtain 78% (g g-1) of reducers sugars released. The maximum concentration of ethanol produced from peel mango by S3 and A1 during 5 days were, respectively 16 and 14.4 g L-1.
  C. Zongo , A. Savadogo , L. Ouattara , I.H.N. Bassole , C.A.T. Ouattara , A.S. Ouattara , N. Barro , J. Koudou and A.S. Traore
  The main goal of this study was to determine the phenol content, the antimicrobial and the antioxidant activities of acetone and water extracts from the rhizomes of Ampelocissus grantii (Baker) Planch (Vitaceae). The polyphenol content of extracts was determined colorimetrically using Folin-Ciocalteu method. The antioxidant activity of the same extracts was also screened using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The antimicrobial activity was performed by disc diffusion and broth microdilution assays against 9 reference bacterial strains including gram-negative and gram-positive bacteria and 1 fungal strain. The acetone extract exhibited the higher level of Total Polyphenol Contents (TPC) which correlated with strong antioxidant activity (IC50 = 10.87 μg mL-1 and AAI = 5.6) and antimicrobial activity. The polyphenol content of the acetone extract was 124.26 gallic acid equivalent/100 g (GAE/100 g), that of water extract was 74.25 GAE/100 g. The largest diameter of inhibition zone (DIZ) (15 mm) and the lowest Minimal Inhibitory Concentration (MIC) value (0.625 mg mL-1) were recorded with Enterococcus faecalis 10907 CIP and Bacillus subtilus ATCC 21332. This study is a scientific base on the use of this plant in traditional health cure system.
  A. Gansane , S. Sanon , P.L. Ouattara , S. Hutter , E. Ollivier , N. Azas , A. Traore , A.S. Traore , I.P. Guissou , I. Nebie and B.S. Sirima
  The aim of the study is to investigate through traditional medicinal plants the possibility for discovery and development of new active and safe antimalarial drugs. For ecological reasons, bark of trunk of Zanthoxylum zanthoxyloides instead to roots was used by traditional healers in Burkina Faso to treat malaria or fever and recent study showed that crude alkaloid extract from the bark of trunk displayed good antiplasmodial activity. The bio-guided chromatographic fractionation of this crude alkaloid extract with solvents yielded 11 semi purified fractions which were tested for their antiplasmodial activity and cytotoxicity, respectively against Plasmodium falciparum W2 strains and K562S cells maintained in continuous culture and using flow cytometer. Non polar fractions 2, 3 and 4 displayed good antiplasmodial activity with IC50 ranging from 1.91 to 4.32 μg mL-1 and little toxicity with selectivity index ranging from 3.03 to 6.15. These data allow further investigations in terms of purification, isolation and development of new antiplasmodial compounds from these semi purified fractions and development of improved phytomedicine.
  J. Koudou , P. Edou , L.C. Obame , I.H. Bassole , G. Figueredo , H. Agnaniet , J.C. Chalchat and A.S. Traore
  The resin oil obtained by hydrodistillation from Dacryodes edulis G. Don growing in Gabon was analyzed by GC and GC/MS. The major constituents in the essential oil were sabinene (21.76%), terpinene-4-ol (19.79%), α-pinene (17.47%) and p-cymene (11.29%). The in vitro antioxidant activity was investigated with two methods: 2, 2-diphenylpicrylhydrazyl radical (DPPH) scavenging essay and β-carotene bleaching test. Butylated hydroxyltoluene was employed as positive control. The essential oil showed antioxidant and DPPH radical scavenging activities and it displayed the inhibition of lipid peroxidation. Furthermore, the antimicrobial activity of essential oil was evaluated using a broth microdilution method. Dacryodes edulis essential oil exhibited antibacterial activity but it was unable to inhibit the growth of fungal species tested.
  I.J.O. Bonkoungou , N. Barro , I. Sanou , L. Toe , M.S. Sanfo , R. Ouedraogo-Traore and A.S. Traore
  The aim of this study was to determine the prevalence of rotavirus infection and characterized group A rotavirus in stool by immunochromatographic test and polyacrylamide gel electrophoresis. Then 150 specimens of stools were collected from patient children between December 2006 and April 2007 and analyzed. The antigenic detection of rotaviruses carry out by immunochromatographic has revealed the presence of group A rotavirus antigen in 21 (14%), adenovirus were also found in 8 (5.33%) and a co-infection rota-adenovirus in 2 (1.33%) of the 150 stool samples tested. Infants were most frequently affected, 15 (71, 43%) of the 21 children rotavirus-positive, were infants ≤ 1 year of age. Based on migration patterns of RNA segments of 21 rotavirus isolates, two distinct groups of electropherotypes of group A rotaviruses were identified: 7 (36.84%) isolates were Long (L) and 12 (63.15%) were Short (S) electrophoretypes but any co-infection by both was identified. This first study in the Burkina-Faso has shown the main genomic patterns of rotavirus. There is a need for further detailed studies on the molecular characterization of rotavirus which would have important implications in vaccine evaluation programs.
  M.D. Traore , V.S.E. Traore , A. Galzi-Pinel , D. Fargette , G. Konate , A.S. Traore and O. Traore
  The roles of guttation fluid, irrigation water, contact between plants and transplantation into contaminated soil in the transmission of Rice yellow mottle virus (RYMV) were assessed. RYMV presence and infectivity were tested by Enzyme-Linked Immunosorbent Assay (ELISA) and by inoculation to susceptible rice cultivar BG90-2. The virus was readily detected in guttation fluid collected from infected rice plants. Transmission tests from this fluid led to high disease incidence (86.6%). Irrigation water collected at the base of infected plants growing in pots was less infectious, as inoculations led to disease incidences below 40%. No virus was detected and could be transmitted from field-irrigation water. Up to 44% healthy rice plants whose leaves were in contact with those of infected plants became infected but, no transmission occurred through intertwined roots. Transplantation of rice seedling into virus-contaminated soil also led to plant infection. However, virus survival in the soil decrease rapidly and infectivity was completely lost 14 days after soil contamination. Altogether, these results indicated that high planting densities of rice are likely to favour secondary spread of rice yellow mottle disease. Transplantation of rice seedlings not earlier than 2 weeks after soil preparation should prevent soil transmission of the virus. Although guttation fluid is highly infectious its contribution to virus infectivity in irrigation water is negligible as field-irrigation water was not found to be an infectious source for RYMV.
  E.F.O. Akomo , C. Zongo , S.D. Karou , L.C. Obame , A. Savadogo , C. Atteke and A.S. Traore
  Because of the resistance of pathogens to actually available drugs, there is a continuous need for new agents to cure several diseases such as malaria and microbial infections. In the establishment of new drugs, plant are often a source of new leads, indeed aqueous, acetone and methanol extracts of Canthium multiflorum were tested for antiplasmodial and antibacterial activities. The antiplasmodial activity was performed on fresh clinical strains of Plasmodium falciparum using light microscopy. The results revealed that the methanol extract was the most active with IC50 of 4.69 μg mL-1. The NCCLS microdilution method performed on clinical and reference strains of pathogenic bacteria yielded MIC and MBC values ranging from 312 to 1250 and 625 to 2500 μg mL-1, respectively. The qualitative analysis of the extract revealed the presence of several chemical groups such as alkaloids, terpens and tannins that might be responsible for the activity of the plant. The issue of this study showed that C. multiflorum is a plant that many attention should be paid to because of it pharmacological potentials.
  L.W. Nitiema , D. Dianou , J. Simpore , S.D. Karou , P.W. Savadogo and A.S. Traore
  An anaerobic digester fed with shea cake rich in tannins and phenolic compounds rich-shea cake and previously inoculated with anaerobic sludge from the pit of a slaughterhouse, enabled six months acclimatization of the bacteria to aromatic compounds. Afterwards, digester waste water samples were subject to successive culture on media with 1 g L-1 tannic acid allowing the isolation of a bacterial strain coded AB. Strain AB was facultatively anaerobic, mesophilic, non-motile, non-sporulating, catalase and oxidase negative bacterium, namely strain AB, was isolated from an anaerobic digester fed with shea cake rich in tannins and phenolic compounds, after inoculation with anaerobic sludge from the pit of a slaughterhouse and enrichment on tannic acid. The coccoid cells occurred in pair, short or long chains and stained Gram-positive. Strain AB fermented a wide range of carbohydrates including glucose, fructose, galactose, raffinose, arabinose, sucrose, maltose, lactose, starch and cellulose. Optimum growth occurred with glucose and tannic acid at 37°C and pH 8. The pH, temperature and salt concentration for growth ranged from 5 to 9, 20 to 45°C and 0 to 15 g L-1, respectively. Strain AB converted tannic acid to gallic acid. These features were similar to those of the Streptococcus genus. The determination of tannic acid hydrolysis end products, ability to utilize various organic acids, alcohols and peptides, GC% of the DNA, the sequencing of 16S rRNA gene and DNA-DNA hybridization will permit to confirm this affiliation and to determine the species.
  L. Ouattara , J. Koudou , D.S. Karou , L. Giaco , G. Capelli , J. Simpore , M. Fraziano , V. Colizzi and A.S. Traore
  The cytotoxic and anti-Mycobacterium tuberculosis H37Rv activities of hydro-alcoholic extract of Lannea acida A. Rich (Anacardiaceae) were assessed. The cytoxicity evaluation was carried out on THP1 monocytoid cell line (after 24 h at 1; 5 and 10 μg mL-1) and showed only a slight modification of lactate dehydrogenase (LDH) release. The rate of monocytes in different stages of mitosis had been amended in absence and presence of extract as follows: Go/ G1 58.83 - 59.83%; synthesis 21.95 - 18.64%; mitosis 16.67 - 15.77%; necrosis 2.65 - 5.64 %. The percentage of inhibition of Mycobacterium tuberculosis proliferation was respectively 77.6 and 36.8% at 1.2 and 0.6 mg mL-1 of extract. This is an interesting experimental study on antimicrobial and immune-stimulating properties of Lannea acida ethanol-water (70%v/v) extract which may contain potential antibacterial and immune-stimulating agents for clinical use.
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