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Articles by A.S. Sadr
Total Records ( 2 ) for A.S. Sadr
  Y. Shojaei Tabar , J. Fayazi , H. Roshanfekr , Kh. Mirzadeh and A.S. Sadr
  Prolactin is a polypeptide hormone with multiple functions, secreted mainly by the anterior pituitary gland this experiment conducted to investigate of prolactin polymorphism in buffalo population in Khuzestan of Iran. DNA was isolated from blood samples of 85 buffalos of different areas. In this study, used Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique for amplified a 156 fragment consisting of part of exon 3. The amplified fragment digested with RsaI restriction endonuclease and subjected to electrophoretic separation in ethidium bromide-strained 3% agarose gel. All the samples studied comprised of single fragment of 156 bp indicating the monomorphic nature of the locus, showing AA genotype.
  B. Abbasi , J. Fayazi , M.T. Beigi Nasiri , H.A. Roshanfekr , Kh. Mirzadeh and A.S. Sadr
  Caseins are a family of milk proteins that approximately constitute 80% of total milk proteins. They are existing in several molecular forms (alpha S1, alpha S2, beta and kappa) with variant alleles of each. The A and B are the most common in cattle breeds. Allele A has threonine (ACC) and aspartic asid (GAT) amino acid at position 136 and 148, respectively by means of point mutation, isoleucine (ATC) substitutes threonine and aspartic acid is substituted by alanine (GCT) and variant A changes to variant B. In this study, the PCR-RFLP method was used for differentiating buffalo population in Khouzestan Province k-CN allels. In order to determaind the level of polymorphism, blood sample were collected from 86 buffalos in Shadegan, Ahwaz, Sosangerd, Dezfoul and Shoshtar cities. DNA extraction was based on Boom method and exon 4 of the kappa casein gene was amplified by specific set of primers for this gene to produce a 453 bp fragment. The amplified fragment were digested with Hinf I restriction endonuclease and then subjected to electrophoresis separation in ethidium bromide-stained 2.5% agarose gel. The results were revealed all buffalo samples were monomorph and genotyped as BB. In this breed, the B allele is only existed, which is reported to be favorable for milk quality and can use for breeding strategies of dairy animals.
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