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Articles by A.S. Sadik
Total Records ( 3 ) for A.S. Sadik
  T.Z. Salem , S.M. El-Gamal and A.S. Sadik
  In this study, an Egyptian isolate of potyvirus known to be zucchini yellow mosaic potyvirus (ZYMV) was isolated from leaves of the cultivar Giza 1 watermelon plants which cultivated in Agricultural Experimental Research Station, Sids, Beni Suef, Egypt. The double antibody sandwich (DAS)-enzyme-linked immunosorbent assay (ELISA) was carried out using polyclonal antibodies specific to ZYMV. The helper component proteinase gene (Hc-pro) of WMV was isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) using primers based on the DNA sequence of ZYMV virus, strain KR-PA. The blast searches identify the Hc-pro sequence as a part of Water Melon Mosaic Potyvirus (WMV). The sequence analysis showed that the isolated Hc-pro gene belongs to a new isolate of WMVs and its DNA sequence and deduced amino acid were compared with the Hc-pro of the French, China and Pakistan isolates. This study showed that the Egyptian isolate is closer to Pakistan isolate than French and China isolates, however, the phylogenetic tree showed that using one gene is not enough to relate these isolates to each other.
  Dalia M. Omar , Elham A. El-Ibiary , A.S. Sadik , Mamdouh H. Abdel-Ghaffar and Badwy A. Othman
  In mid-February 2006, an outbreak of Highly Pathogenic Avian Influenza (HPAI) H5N1 affected the commercial poultry production sector and backyards in Egypt and resulted dramatic economic losses to the poultry industry of Egypt and continue to pose a serious threat to public health. The present study was designed for detection and isolation of Avian Influenza Viruses (AIV) circulating among poultry since their first detection in 2006. Tracheal and cloacal swabs were taken from the freshly dead birds. Till now (2010) the tested swab samples were inoculated into the allantoic cavity of 9-11-day-old SPF Embryonated Chicken Eggs (ECE) for virus isolation. The allantoic fluids (AF) of the inoculated ECE were examined for detection of Avian Influenza (AI) isolates using rapid haemagglutination test (HA). It was found that, all the inoculated isolates caused high mortalities up to 100% for the embryos 24-48 h post inoculation and gave very strong RBCs agglutination. The haemagglutination positive samples were identified and subtyped antigenically by serological tests using Haemagglutination Inhibition test (HI) using standard AI antisera and genetically by RT-PCR using specific primer. All the isolates were confirmed to be H5N1 and grouped according to the year of isolation as 2006, 2007, 2008, 2009 and 2010 group isolates. The HA titers of the above mentioned isolates were 6, 7, 8, 7 and 7, respectively. All the isolated AI viruses were titrated in ECE and examined for determination of their pathogenicity in Specific Pathogen Free (SPF) 4-6 week old chicken. All the AI isolates proved to be HPAI viruses where the Intravenous Pathogenicity Index (IVPI) score for them were 2.1, 2.5, 2.3, 2.2 and 2.3 and their titers in ECE were 10.1, 9, 9.3, 9.3 and 10 Log10 EID50/mL for 2006, 2007, 2008, 2009 and 2010 isolates, respectively.
  Eldessoky S. Dessoky , Mohammed Alqurashi , Saqer S. Alotaibi and A.S. Sadik
  Background and Objective: Pomegranate is grown for its rich flavour in numerous tropical and subtropical areas, like Egypt and the Kingdom of Saudi Arabia (KSA). Assessing the genetic background of the pomegranate is the key to its expansion through the Middle East, where tissue culture reproduction strategies could be used to solve environmental and economic problems. This study aimed at studying the genetic stability of 2 pomegranate genotypes in vitro micro-propagated in the Kingdom of Saudi Arabia by using the random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) and inter simple sequence repeats (ISSR) tools. Materials and Methods: The two above mentioned molecular tools were used to evaluate the DNA fingerprints of Taify and Yemeni pomegranate genotypes 12 weeks post in vitro propagation in Taif, Kingdom of Saudi Arabia compared to the mother plant. Shoot tip explants of 4-5 cm long were grown on Murashige and Skoog (MS) medium supplemented by 1.0 mg L1 NAA, 2.00 mg L1 IBA and 2 g L1 activated carbon for 4 weeks for rooting. On 12 weeks DNA extracts were prepared from the acquired plantlets obtained and used as templates for each of RAPD-PCR and ISSR tools. Results: The RAPD-PCR and ISSR assays generated a total of 79-94 and 57-72 DNA fragments, respectively. In case of RAPD-PCR 80 and 90% of the primers used and developed monomorphic fragments of the Yemeni and Taify genotypes, respectively, particularly OPG08 primer for Taify genotype and OPA04 and OPD07 primers for the Yemeni genotype. Regarding ISSR, no DNA polymorphic for the micropropagated clones were recorded compared to the mother plant. Conclusion: The ISSR assay’s findings indicated the genetic homogeneity between the in vitro micropropagated clones of both pomegranate genotypes and the mother plants.
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