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Articles by A.R. Varasteh
Total Records ( 2 ) for A.R. Varasteh
  R. Majidzadeh Heravi , L. Roozbeh Nasiraii , M. Sankian , H. Kermanshahi and A.R. Varasteh
  To date, lactobacilli are widely used in food industries and new probiotic products; hence these are considered as an attractive target for genetic modifications. This study was conducted to improve electroporation of probiotic lactobacilli which is a necessary prerequisite for genetic manipulations. Four strains of probiotic lactobacilli from different sources were grown in Man Rogosa and Sharp (MRS) broth medium containing glycine, as a cell wall weakening agent or a pulse of glycine for evaluation of glycine effect on electroporation efficiency. After evaluation of various parameters such as washing buffers, washing times, electric field strength, pulse duration and plasmid concentration, a practical electroporation protocol was presented to improve electrotransformation of lactobacilli in comparison with two standard protocols. This new protocol exhibited higher transformation efficiency (transformants/μg plasmids) than standard methods (p<0.05) with no differences between species (Lb. casei, Lb. crispatus, Lb. salivarus and L. rhamnosus). The pulse of glycine had no effect on the number of the transformants in three methods. In contrast to plasmid concentration, plasmid size had no influence on the transformation efficiency. The modified method enabled to transform plasmid into the resistant lactobacilli against transformation. These bacteria have potential for bioengineering research to improve special performance.
  R. Falak , M. Sankian and A.R. Varasteh
  During the last few decades, the prevalence of food allergies has been increased dramatically. Concurrently organophosphate pesticides have been increasingly used in agriculture and in select industries. It seems that these organic agents might perpetuate the clinical severity of allergic manifestations in atopic patients, or even modify the epigenetic control of allergen expression in plants. In addition to altered allergen expression, these tiny molecules may directly bind to non-allergenic proteins and potentially modify their epitopes-now rendering these proteins allergenic. This type of change would be along the lines of how certain metal pollutants (like chromium) can modify proteins in the skin after dermal contact and give rise to ‘allergic’ reactions in the exposed host. Lastly, the organophosphate pesticides might also lead to increases in the production of polyamines in fruits and cause pseudo-allergic reactions. This review analyzes some evidence regarding possible mechanisms of pesticide-induced allergenicity of plant proteins.
 
 
 
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