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Articles by A.R. Omar
Total Records ( 5 ) for A.R. Omar
  Elawad A. Hussein , M. Hair-Bejo , A.R. Omar , Pit S. Liew , Mohammed Ibrahim Saeed , Siti S. Arshad , Elmutaz A. Awad and I. Aini
  Background and Objective: There are 3 pathogenic strains of infectious bursal disease virus (IBDV), which are classical, variant and very virulent strains. The objective of this study was to design a scoring system for lesions induced by different strains of infectious bursal disease virus in chicken. Materials and Methods: Three experiments were conducted. In experiments 1 and 2, chickens were divided into infected and control groups. Infected groups of experiments 1 and 2 consisted of 15 and 24 specific pathogen-free (SPF) chickens, respectively. Infection was induced by oral administration of 107.5 50% EID50/0.1 mL of very virulent IBDV (vvIBDV). Infected chickens in experiment 1 were euthanised by cervical dislocation on days 1, 2, 3, 4 and 5 post-inoculation (pi). Infected chickens in experiment 2 were euthanised at hours (h) 2, 4, 6, 12 and days 1, 2, 4 and 6 pi. Control groups in experiments 1 and 2 consisted of 6 and 15 SPF chickens, respectively. Chickens of the control group in experiment 1 were euthanised on days 1 and 5 pi, whereas control group chickens in experiment 2 were euthanised on days 0, 1, 2, 4 and 6 pi. Then, 20 SPF chickens, in experiment 3, were divided into three groups; in the first group, 10 SPF chickens were infected with vvIBDV, in the second group, 5 SPF chickens were infected with classical IBDV (caIBDV) (103.0 EID50/0.1 mL) and the third group of chicken was kept as a control group without infection. Five chickens from first group, five chickens of second group and five chicken of the third group were euthanised on day 4 pi. Another 5 chickens from first group were euthanized on day 7 pi. In all previous experiments, tissues of bursa of Fabricius, caecal tonsil, liver, kidney, spleen, junction of proventriculus and gizzard, intestine, muscle and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin and sectioned. HS staining was applied. Results: When the strain of the virus was vvIBDV, the scoring was always greater in comparison with the strain caIBDV. The lesions induced in the bursa of Fabricius by vvIBDV strain, when the infection was chronic, were determined. Conclusion: A scoring system for the lesions induced by different strains of IBDV in 9 different tissues was expected to be beneficial in the field of histopathology.
  B.Y. Takele , S Khairani-Bejo , A.R. Bahaman and A.R. Omar
  Brucellosis poses a significant animal and public health problem in many developing countries and requires fast and accurate diagnosis. A PCR assay amplifying part of the Brucella melitensis specific IS711 gene was developed and applied to mice clinical samples with experimental trial. Over an 8 week period of infection, whole blood and serum were examined from 78 experimental mice, with a total of 60 samples from B. melitensis infected mice and a group of 96 control samples from mice inoculated with Brucella abortus 544, Yersinia enterocolitica O:9 and Brucella broth. Regardless of date of infection, the sensitivity of whole blood and serum based PCR assay with samples from B. melitensis infected mice was found to be 100% (30/30) and 83.3% (25/30), respectively. Serum samples collected at 60 days post infection (p.i) of B. melitensis failed to show positive result. An amplicon of 252 bp was obtained in all PCR positive samples. All samples obtained from the control groups tested negative, conferring an assay specificity of 100%. These results show that though, use of serum-PCR may lead to assay simplification and shorten turnaround time, but the optimal clinical specimen for this test was not serum but whole blood, which leads to maximum assay sensitivity.
  Maged Ahmed AL-Garadi , S. Khairani-Bejo , Z. Zunita and A.R. Omar
  Isolation of Brucella melitensis is the standard gold of identification and confirmation of animal brucellosis. However in Malaysia, Brucella sp., infection of goat was increasing recently and there is no evidence for diagnosis of the serover of Brucella sp., that cause the disease in goat population except the detection of serological methods. Isolation and identification of Brucella melitensis have been done by bacteriological methods in addition to conventional Polymerase Chain Reaction (PCR) and Real-time PCR for detection of Brucella melitensis from samples collected from vaginal swabs on suspected farm. In conclusion, four isolate have been got out of 300 vaginal samples and all isolate is belong to Brucella melitensis server 1. The Real-time PCR is the easy and save method for confirmation of brucellosis in goats population.
  Maged Ahmed AL-Garadi , S. Khairani-Bejo , Z. Zunita and A.R. Omar
  PCR assays have been shown to be a promising option for the diagnosis of brucellosis. However, there is no study conducted in Malaysia to identify the brucellosis in goat’s population. In this study three whole blood samples and sera were collected from goat’s farm in Kedah state Malaysia which was suspected to have brucellosis. Serological and molecular detection of brucellosis have been done including RBPT, CFT, conventional PCR and Real time. The evaluation of each test have been discussed rather than the sensitivity and specificity of the each test which can be used in Malaysia national eradication programs. In conclusion, the combination between the serological test and molecular technique specially real time PCR depend on IS711 region in hypothetical protein is promising and can be reduced to false positive result which can cause heavy economical loss during controlling programs.
  I.M. Ahmed , S. Khairani-Bejo , L. Hassan , A.R. Bahaman and A.R. Omar
  The potential diagnostic ability of Recombinant Outer Membrane Proteins (rOMPs), a combination of equal concentrations of rOMP25, rOMP28 and rOMP31of Brucella melitensis was investigated using Enzyme-Linked Immunosorbent Assay (ELISA) to differentiate the False Positive Serological Reactions (FPSR) in the serological diagnosis of caprine brucellosis. The rOMPs was tested using sera from three groups of goats with known Brucella exposure status which represent, naturally B. melitensis infected goats (infected), Brucella free goats (non-infected) and goats vaccinated with B. melitensis Rev. 1 vaccine strain (vaccinated). Additionally, all the sera were tested using the common serological tests which are Rose Bengal Plate Test (RBPT), BRUCELISA-400SG and Complement Fixation Test (CFT). When testing infected and non infected groups, the rOMPs I-ELISA recorded 94.44% (34/36) sensitivity and 100% (36/36) specificity and this almost agreed with the results obtained from testing the same serum samples using RBPT, BRUCELISA-400SG and CFT. However, when goats vaccinated with B. melitensis Rev. 1 vaccine strain were tested by the common serological tests, RBPT, BRUCELISA-400SG and CFT they wrongly recorded positive results for all the tested serum samples (26/26). While the developed rOMPs I-ELISA was able to differentiate the vaccinated from infected animals with 94.44 sensitivity and 84.62% specificity. The potential diagnostic ability of rOMPs would be of great importance as serologic marker to minimize the FPSR in eradication programs of caprine brucellosis.
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