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Articles by A.R. Khosravi
Total Records ( 2 ) for A.R. Khosravi
  A.R. Khosravi , M. Riazipour , H. Shokri , M.L. Mousavi and M. Mahmoudi
  The aim of the study was to evaluate the protein similarity degree among 15 C. albicans isolates with different virulence obtained from healthy and infected human and animals. Yeast cells were grown in YPG medium and collected by centrifugation. After cell wall disruption, the cell wall and cytoplasmic proteins were submitted to PAGE and SDS-PAGE techniques. Similarity degree was determined using the Dice similarity coefficient (SD). Candida albicans isolates showed high similarity together with average 86.2% (78.1<SD<100). Regarding to the virulence and host aspects, the means SD of high virulent and low virulent isolates as well as human and animal isolates were 85.8 and 85.6% in PAGE, respectively. Considering SDS-PAGE, electrophoregrams of C. albicans cytoplasmic proteins of low and high virulent isolates as well as human and animal isolates showed the similarity about 90%. The mean SD of cell wall was 91.9% between low virulent and high virulent isolates and 92.2% between human and animal isolates. Such results suggest a highly protein similarity degree among the most isolates with low and high virulence as well as from human and animal hosts. The whole-cell protein profile obtained by these techniques could provide additional criteria for the serologic and immunologic studies of C. albicans.
  S.A.A. Sefidgar , Gholampour Azizi , A.R. Khosravi and S. Roudbar-Mohammadi
 

In this study, raw cow milk samples were collected from milk churns at 40 traditional and semi-industrial cattle farms located in Babol (Northern Iran) in winter 2006. In total, 120 raw milk samples were tested for Aflatoxin M1 (AFM1) contamination by competitive ELISA. In 68 out of 120 samples (56.7%) the presence of AFM1 was detected by concentration ranging from 50 to 352.3 ng L-1. Fifty two samples (43.3%) contained AFM1 at levels of 4-50 ng L-1 (The AFM1 contamination levels were between 4-352.3 ng L-1 by the average of 102.73 ng L-1). In general, 56.7% of samples were beyond the limit of European community regulations (50 ng L-1). In other words, the AFM1 contamination levels in raw milk were more than twice over standard levels. The AFM1 contamination levels, (>50 ng L-1), in January, February and March were 40, 65 and 65%, respectively. Estimation of contamination of AFB1 using AFM1 in feed stuff showed that it was about 0.25 to 22 µg kg-1 holding the average of 46.7%, being higher than European community limit (5 μg kg-1).

 
 
 
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