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Articles by A.R. Raha
Total Records ( 7 ) for A.R. Raha
  S.O. Sarah , M. Zamri-Saad , Z. Zunita and A.R. Raha
  Pasteurella multocida has been recognised as an important pathogen of animals that causes a wide spectrum of diseases, collectively termed as pasteurellosis. The bacteria possess several housekeeping genes that maintain the viability of the bacteria. One of the housekeeping genes is the gdhAgene that serves as a major link between carbon and nitrogen metabolism. The full length gdhA gene of P. multocida B:2 was found to be 1180bp while the functional fragment of the gene was 652bp. The gene was successfully sequenced and later cloned into E. coli pCR 2.1-TOPO vector.
  N. ALHaj , N.S. Mariana , A.R. Raha and Z. Ishak
  A PCR for detection of two categories of diarrheagenic Escherichia coli was developed. This method proved to be specific and rapid in detecting virulence genes from enteropathogenic E. coli (EPEC) (eae and bfp) and Shiga toxin-producing E. coli (STEC) (stx1, stx2 and eae) from seventy isolates of various sources. Present results confirm that it is possible and feasible to perform a simultaneous amplification of the virulence genes from two categories of diarrheagenic E. coli (STEC, EPEC) and that this technique becoming a novel diagnostic tool for future water food-borne outbreaks studies.
  Yazid A.M. M. Shuhaimi , A.M. Ali , A.R. Raha , R.R. Ernie Eileen , H. Rowina and N.A.N. Abdullah
  A total of 8 bifidobacteria strains were isolated from faeces of breast-fed infants. They were identified as Bifidobacterium breve (2 strains) and B. infantis (6 strains). Almost all strains were resistant to amino glycoside antibiotics, but sensitive to chloramphenicol, nitrofurantoin and erythromycin. Susceptibility to other antimicrobial was varied. Four strains shown to have plasmids DNA with band size 50, 8 and 2.3 kb.
  B.C. Yiap , C.M.V.L. Wong , M.Y. Hirzun and A.R. Raha
  DSN1 is a SPB component that functions in yeast chromosome segregation process. It has been shown to be of haploinsufficient but its nature of gene dosage sensitivity was yet to be established. This study indicated that gene dosage variation of it has caused abnormalities in both cellular growth and nuclear division. Alteration of cell size and compromised budding index were part of the phenotypic manifestations. Changes in gene copy number also led to nuclear division and separation errors such as aneuploidy and nuclear disjunction. The results of this study provide direct evidence of the function of DSN1 in chromosomal segregation process. Gene dosage sensitivity of the gene also implied that its gene product would be forming complex with another protein.
  Nagi A. ALHaj , N.S. Mariana , A.R. Raha , W.M.Z. Somarny , S.L.L. Suang and F.Z. Hana
  Bacterial lipopolysaccharides are the major outer surface membrane components present in almost all Gram-negative bacteria and act as extremely strong stimulators of innate or natural immunity in diverse eukaryotic species ranging from insects to humans. The DNA sequence of the O-antigen biosynthesis cluster of a putative probiotic and pathogenic strain, Vibrio alginolyticus has been determined. Here, we report the sequence of the LPS biosynthesis genes, wzm, wzt and wbil and the analysis of the genes using Biology Workbench 3.2. From the study, it shows that the sequences of LPS genes in V. alginolyticus are highly homologous to the LPS genes in Vibrio cholerae isolates with more 80% homology. However, several variants of the wbil sequence have been found in the V. alginolyticus isolates compared to the other genes, wzm and wzt.
  N. ALHaj , N.S. Mariana , A.R. Raha and Z. Ishak
  Diarrhea caused by multidrug-resistant bacteria is an important public health problem among children in developing countries. Escherichia coli is an important cause of disease in animals and humans worldwide. Twenty five E. coli isolates with rate 61.2% among human and environments were tested for susceptibility to 10 antimicrobial agents by disk diffusion method. Resistant isolates were screened by molecular methods for resistance genes, TetA, TetB, strepA, MarI and MarII. Molecular result showed that all isolates harbored resistance gene for the TetA, TetB, strepA, MarI, and MarII even though the genotypic test showed sensitive to the drugs. E. coli isolates exhibit a wide repertoire of genetic elements to sustain antimicrobial pressure. The results of this study using pheno-genotypic techniques highlight the distribution of E. coli among human, animal, aquatic ecosystems and the potential public health threat of E. coli originating from municipal wastewater sources.
  N. ALHaj , N.S. Mariana , A.R. Raha and Z. Ishak
  Antimicrobial agent resistance has been recognized as an emergingworldwide problem in both human and animals, antimicrobial agent use is considered the most important factorfor the emergence, selection and dissemination of antimicrobialagent-resistant bacteria, intrinsically either acquires the resistance gene from other bacterial environment or development of pumping out mechanism. The aim of this study was to generate baseline dataon the prevalence of antimicrobial resistancein Escherichia coli isolates from different sources. Seventy E. coliisolates from humans and environments were tested for susceptibility to 10 antimicrobialagents by diffusion method. Resistance was found in 61.2 % of the isolates. The most prevalent resistanceswere to kanamycin and tetracycline (81.4%),followed by chloramphenicol (75.7%) and gentamicin,(74.3%). The low prevalent were to cefetoxin (44.3%), norofluoxacin (27.1%) and ciprofluoxacin (24.3%). This study showed the distribution of antimicrobial agent resistancein E. coli isolates from a variety of sources and analysisof such patterns of resistance may prove to be useful beyondsimple description. Regarding to the concern of water quality and environmentalcontamination by human and agricultural waster have increased,it has become increasingly important to develop low-cost screeningtools that can be used to identify the most probable sourceof contamination.
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