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Articles by A.R. Hanan El-Hosieny
Total Records ( 1 ) for A.R. Hanan El-Hosieny
  M.N.M. Helaly and A.R. Hanan El-Hosieny
  The present investigation aimed to study the effects of NaCl salinity on immature ovule regeneration of some Citrus genotypes. The relative combination effects of NaCl salinity with different sweet orange genotypes were evaluated during the developmental stages of their micropropagation. Somatic embryogenesis was achieved in callus media of nucellus derived from undeveloped ovules of 6 immature fruits of sweet orange genotypes [Citrus sinensis (L.) Osb] using modified MS media supplemented with malt and Ascorbic Acid (AsA) under different NaCl salinity levels. Complete plantlets were developed from the somatic embryos by further subculturing in the same specific media depending on the genotypes used and the level of NaCl. The combined data show that the values of genotype variance were positive but less than environmental media variance for the studied levels of salinity. In vitro seedlings of sweet orange genotypes accumulated more organic solutes (total amino acids, proline, glybet, total sugars and total organic acids) than those of the corresponding genotypes in vivo seedling having the same physiological age under different salinity levels. Moreover, in vitro seedlings scored lower level of lipid peroxidation and higher activities of Superoxide Dismutase (SOD), Peroxidase (POX), Ascorbate Peroxidase (APOX), Catalase (CAT), Glutathione Reductase (GR) than in vivo seedlings. Thus, in vitro seedling exhibited more salt tolerant than the relatively sensitive in vivo plants of sweet orange Citrus. It could be concluded that Tissue Culture Technique (TCT) in the presence of MS media supplemented with malt and/or AsA improved salt tolerance of Citrus; sweet orange genotypes by protecting the protein turnover machinery against stress damage and up-regulating stress protective proteins.
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