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Articles by A.P. Lipton
Total Records ( 4 ) for A.P. Lipton
  M. Thangaraj , A.P. Lipton and T.T. Ajith Kumar
  Seahorse populations are declining year by year not only in India but also throughout the world, because of over-fishing and increasing demand in Chinese market. The yellow seahorse, Hippocampus kuda is one of the dominant species and distributed all along the Indian coast. To increase its population in the wild, captive breeding and sea ranging is the alternative option. To breed this fish, it is very essential to know about its reproductive behavior. During this one year study, the clutch size of Hippocampus kuda varied from 61-325 depending on the maternal body mass. Size and weight of individual egg were also dependent on maternal body mass. However, there was no significant correlation noted between paternal body mass and brood size suggesting dominant role of females in determining brood size only. But the long and healthy babies were produced by larger males.
  M. Thangaraj , Vishruth Prem , T. Ramesh and A.P. Lipton
  The aim of the study was to find the RAPD finger printing of Pseudomonas aeruginosa, Bacillus circulans and Serratia marcescens, using ten random primers. The RAPD-PCR produced reproducible electrophoretic band patterns only in seven primers. There were 67 species specific bands observed, among these, 25 bands in P. aeruginosa, 19 in B. circulans and 23 in S. marcescens. This study suggests that RAPD typing could be an additional rapid typing method for studying the epidemiology of these pathogens. Based on the RAPD data the genetic identity between S. marcescens and B. circulans was more (0.3803) than the other.
  S.K. Subhash and A.P. Lipton
  Commercialization of pearl culture requires large scale hatchery production of larvae. The survival of larvae depends on stocking density, environmental factors, diets and diseases. The results of experiments using 4 stocking densities at the rate of 100, 1000, 2000 and 3000 larvae L-1 of filtered sea water indicated highest survival of 15.8 and 10.5% in the low stocking densities followed by 1.4 and 0.34%, respectively in other 2 stocking densities at an ambient temperature of 28.6±0.4°C. Although, the larvae were fed with Isochrysis galbana at the recommended cell densities, a positive correlation between increased bacterial load and pearl oyster larval survival was recorded in all the stocking densities. Eventhough, total number of spat produced in 100 and 1000 larvae L-1 stocking density was more or less similar and considering the management strategies including man power, cost of production of microalgal culture and infrastructure facilities such as availability of tanks and space in an established hatchery the stocking density of 1000 larvae L-1 will be optimum for commercial hatchery production of pearl oyster larvae.
  P. Kavitha , A.P. Lipton , A.R. Sarika and M.S. Aishwarya
  An archeon Haloferax volcanii KPS1 was isolated from Kovalam saltern, Kanyakumari, India. It produced halocin which was active against gram positive and gram negative bacteria viz., Bacillus subtilis, Streptococcus mutans, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, respectively. The halobacteria H. sodomens was inhibited to the maximum by the halocin KPS1 of the Haloferax volcanii KPS1. The growth and halocin production was maximized when the organism was grown at a temperature of 40°C at the pH of 7.0 and when supplemented with 25% w/v NaCl to the halophilic broth medium. The results of the stability studies indicated that the halocin KPS1 became thermolabile at the temperature >80°C and was stable over a wide range of pH from 3.0-9.0. A loss of activity of halocin KPS1 was detected when treated with proteolytic enzymes like proteinase K and trypsin indicating the proteinaceous nature of the antibacterial compound. The halocin produced by Haloferax volcanii KPS1 would provide a lead for developing antimicrobial drugs.
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