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Articles by A.O.T. Ashafa
Total Records ( 4 ) for A.O.T. Ashafa
  A.O.T. Ashafa , D.S. Grierson and A.J. Afolayan
  The effect of various methods of drying on the content and chemical quality of the essential oil of this herb was studied. The most prominent components in the fresh, air-dried, sun-dried and oven-dried oils extracted by hydrodistillation were the monoterpenes, α-pinene (9.1, 7.4, 10.2 and 6.8%), β-pinene (3.5, 3.6, 9.6 and 7.0%), myrcene (18.7, 18.4, 22.0 and 16.4%), limonene (26.5, 26.5, 24.4 and 27.4%), cis-ocimene (2.2, 1.9, 2.8 and 2.0%), trans-ocimene (4.8, 5.6, 12.4 and 11.6%) and α-terpineol (3.4, 2.7, 2.2 and 3.2%). Methyl-eugenol, β-caryophyllene, trans-farnesene and bicyclogermacrene were the major sesquiterpenes and all were in minute quantity. Cis-lachnophyllum ester (16.2, 18.1, 19.2 and 16.1%, respectively), was the dominating non-terpenoid polyacetylinic compound. The different drying methods have no significant effect on the quality and composition of essential oil from F. muricata.
  A.O.T. Ashafa , D.S. Grierson and A.J. Afolayan
  The essential oil was extracted by hydrodistillation. A total of thirty-eight compounds were identified with α-pinene (9.1%) β-pinene (3.5%), myrcene (18.7%), limonene (26.5%), cis-ocimene (2.2%), trans-ocimene (4.8%) and terpineol (3.4%) as the major monoterpenes, while, cis-lachnophyllum ester (16.2%) was the major non-terpenoid polyacetylenic compound. The antibacterial activity of the oil was investigated against 16 bacterial strains using broth microdilution method. The oil inhibited all the test organisms with more pronounced activity on Gram-positive than the Gram-negative bacteria. The Minimum Inhibitory Concentration (MIC) for Gram-positive bacteria range from 0.08-2.50 mg mL–1, whereas, it was 0.08-5.00 mg mL–1 for the Gram-negative bacteria. The ability of the oil from F. muricata to inhibit a range of nosocomial pathogenic bacterial strains at a concentration less than that of streptomycin makes the oil a candidate for possible development of antibiotic drug.
  A.O.T. Ashafa , D.S. Grierson and A.J. Afolayan
  The antimicrobial activities of the acetone, methanol and water extracts from the leaves, stems and roots of this herb were investigated against 10 bacterial and 5 fungal species using the dilution method on solid agar medium. The acetone extracts from the leaf and root were active against Gram-positive bacteria with Minimum Inhibitory Concentration (MIC) ranging between 1.0 and 7.0 mg mL-1, whereas the acetone stem extract was able to inhibit all the bacterial strains at 0.5-7.0 mg mL-1. The methanol extracts of the 3 plant parts showed activity against all the bacterial isolates with MIC values ranging between 0.1 and 10.0 mg mL-1. Again all the extracts exhibited appreciable activity against all the fungi species investigated. The methanol extract of the root showed 100% inhibition against Aspergillus niger, A. flavus and Penicilium notatum at 5 mg mL-1 while it was 88.61% inhibition in Mucor hiemalis. The ability of the extracts of F. muricata to inhibit the growth of several bacteria and fungi is an indication of its broad-spectrum antimicrobial potential which may be employed in the management of bacterial and fungal infections.
  A.O.T. Ashafa , D.S. Grierson and A.J. Afolayan
  The foliar micromorphology of Felicia muricata (Thunb.) Nees (Asteraceae) was observed with the JEOL (JSM-6390LV) Scanning Electron Microscope (SEM). Both the abaxial and adaxial surfaces were characterized by anisocytic stomata which were more prevalent on the abaxial surface than the adaxial surface. The leaves have only one type of multicellular non-glandular trichomes that are long and cylindrical, tapering to a sharp point and running parallel to the leaf surface in the direction of the apices. Crystal deposits were also observed on the surfaces of the leaves near the stomata. Energy dispersive X-ray spectroscopy-SEM shows that Na, Al, Si, and K were the major constituents of the crystal analyzed. Since no glandular trichomes were present on the leaves of this herb, the bioactive components present in this plant may be produced in some other tissues in the leaf other than the trichomes.
 
 
 
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