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Articles by A.O. Eni
Total Records ( 2 ) for A.O. Eni
  F.Y. Daramola , J.O. Popoola , A.O. Eni and O. Sulaiman
  Vegetable crops are highly susceptible to a wide range of pests and diseases among which are the root-knot nematodes (Meloidogyne species). Traditionally, identification of Meloidogyne species had been based on use of morphological characters such as the perennial pattern. In recent times, accuracy of nematode identification using only morphological tools has been challenged due to similarities in morphological characters of some nematode species. The aim of this study was to identify the root-knot nematodes associated with some vegetable crops cultivated on Covenant University farm, Ota, South-west, Nigeria using molecular tools and to determine the population densities of Meloidogyne spp. on the selected vegetable crops. Plant-parasitic nematodes were extracted from soil and roots of Abelmoschus esculentus, Celosia argentea and Corchorus olitorius cultivated on Covenant University farm. The nematode species were identified and counted under a compound microscope. The molecular characterization of the Meloidogyne species was done using single adult female nematodes and eggs which were picked out for DNA extraction and amplified with specie-specific primers through Polymerase Chain Reaction (PCR) and separated on 0.5% agarose gel. High population of plant- parasitic nematodes was recorded on the vegetable crops cultivated on covenant university farm. Also significantly higher population (p<0.005) of Meloidogyne spp. were found in association with C. argentea and Corchorus olitorius than those recorded on Abelmoschus esculentus from the vegetable farm. The molecular characterization of the Meloidogyne species from the farm indicates Meloidogyne incognita as the nematode species associated with the vegetable crops.
  S. Oranusi , F. Omagbemi and A.O. Eni
  The microbial quality of snacks (ready to eat foods) sold in Ota, Ogun state was investigated. A total of 100 different samples from 3 vending sites namely, a university cafeteria, a top class snacks bar and a local kiosk were analyzed for total aerobic plate count, coliform count and for specific pathogens and fungi. The university cafeteria had mean total aerobic plate count and coliform count ranging from 1.1x103-3.0x104 and 1.0x102-2.2x103. The snacks bar had mean total aerobic plate count and coliform count ranging from 2.0x103-5.8x105 and 1.4x102-1.8x105 while the local kiosk had mean total aerobic plate count and coliform count ranging from 2.1x103-5.4x105 and 1.0x102-8.0x104, respectively. The fungal counts from the three sites are within 1.0x102- 4.0x102. Six different bacterial and three fungal isolates were identified to include E. coli, S. aureus, Bacillus cereus, Enterococcus, Klebsiella sp., Pseudomonas sp. and Aspergillus niger, Penicillium sp. and Mucor. The presence of E. coli and Enterococci which are indicator organisms call for concern. Adoption of good manufacturing practice and Hazard Analysis Critical Control Point (HACCP) are necessary to preventing occurrence of food borne illness.
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