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Articles by A.O. Adebambo
Total Records ( 3 ) for A.O. Adebambo
  O.T. Agbebi , S.O. Sofela , A.O. Adebambo and M.O. Awodiran
  Electrophoresis of myofibrillar and sarcoplasmic muscle proteins of African catfish: Clarias gariepinus, Heterobranchus bidorsalis and their reciprocal hybrids in South-West Nigeria was carried out to reveal the similarities and dissimilarities among species in order to aid the selection of suitable strains for aquaculture that could lead to production of new varieties of fishes to alleviate the problem of short supply of fast growing quality fish seeds. The study was aimed at analyzing the muscle protein profiles of C. gariepinus, H. bidorsalis and their reciprocal hybrids. Sixteen juveniles fish samples (comprising four samples from each mating combinations) artificially propagated and reared for sixteen weeks were analysed electrophoretically. Myofibrillar and sarcoplasmic fractions were prepared by homogenizing 150 mg of fish muscle in 1.5 mL of rigor buffer containing 10 mM Trismeleates, 60 mM KCl, 5 mM MgCl2, 1 nM EDTA. Extracts were centrifuged in a tube at 10,000 g for 5 min at 4°C. The resultant pellets (myofibrilla) and supernatant (sarcoplasmic) were separated using 12.5% Sodium Dodecyl-Sulphate Polyacrylamide Gel Electrophoresis (1D SDS-PAGE). The relative concentration of individual protein bands were analysed using TotalLab™ 1D software. The individual protein bands in the electrophoregram were identified in relation to their molecular weights. A total of eleven and seven protein bands were resolved in myofibrilla and sarcoplasmic fractions, respectively. The 5th band with Molecular Weight (MW) of 52.23 kDa of the myofibrilla electrophoregram distinguished C. gariepinus from H. bidorsalis while the 3rd band with MW 119.04, 4th band with MWs 101.49 and 102.13; 8th band with MWs 29.24 and 29.39 kDa distinct the pure breeds from the hybrids. However, in sarcoplasmic fraction, the 3rd and 5th bands with MWs 92.11 kDa and 54.28 kDa, respectively distinguished the hybrids in the while the 7th band with MW 41.67 kDa distinct the pure breeds. Therefore, this research will serve as a bridge between the existing gaps of information available on the muscle protein profile of C. gariepinus, H. bidorsalis and their reciprocal hybrids and the study identifies the proteomic classification of clariid species with the aim of enlightening fish researchers and aquaculturist on the characterization of broodstock selection for successful breeding exercise.
  A.O. Adebambo , V.A. Mobegi , J.M. Mwacharo , B.M. Oladejo , R.A. Adewale , L.O. Ilori , B.O. Makanjuola , O. Afolayan , G. Bjornstad , H. Jianlin and O. Hanotte
  Genetic diversity studies that utilize phenotypic and genetic information are informative when formulating breeding and conservation plans. The present study utilizes sequences of mitochondrial DNA (mtDNA) D-loop region of 232 village chickens from Southern and Northern Nigeria to determine the origin and diversity of Nigerian local chickens. Thirty-six polymorphic sites which generate 35 haplotypes are identified. Phylogenetic analyses group Nigerian local chickens to a single clade and 97.8% of the total maternal variation occurs within populations. Reference sequences representing the major chicken mtDNA lineages from Asia indicate the Indian subcontinent to be the likely main center of origin of Nigerian village chicken. Lack of phylogeographic structure among Nigerian village chickens suggest extensive genetic intermixing within the country.
  A.O. Adebambo , M.A. Adeleke , M. Whetto , S.O. Peters , C.O.N. Ikeobi , M.O. Ozoje , O.O. Oduguwa and Olufunmilayo A. Adebambo
  Two hundred and ninety five cocks and two hundred and ninety nine hens were selected from a diallel combination of four breeds of chickens; [Anak Titan (A), Alpha (B), Giriraja (G) and Normal indigenous (N) chickens] at 12 weeks of age in a broiler improvement program for carcass analysis. The following data were collected in percentages: economically important traits = Live weight (g), Plucked weight, Eviscerated weight, Carcass yield, Abdominal fat percentage, Breast yield, Thigh yield, Drumstick yield; survival organs = Wing yield, Internal organ, Empty gizzard yield, Heart yield, Lung yield, Kidney yield, Liver yield. Analyses of variance of carcass traits show that sire and dam genotype significantly (p<0.05) affected carcass traits. Anak Titan sires and dams performed best in economically important traits, while N and B performed better in survival organs. Sex had significant (p<0.05) effect only on live weight with cocks having higher values of 979.55±56.62 and hens 879.6±34.18. Results of diallel analysis to test for general and specific combining abilities of breeds on traits showed that additive genetic effects were important in determining economically important traits, indicative that improvement can be achieved by selection. Dominance effects were important in control of survival organs, indicative of improvement by crossbreeding. Estimates of GCA for carcass traits show that Anak Titan had highest general combining ability for most of the carcass parameters while the least values were found among Alpha chickens. Estimates of SCA for carcass parameters showed AN cross generally had highest SCA for most of the carcass traits. Least SCA values for carcass parameters were generally recorded for AB crosses. It is recommended that an improvement process that involves all the breeds should be adapted using reciprocal recurrent selection or modifications of it.
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