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Articles by A.N.M. Fakhruddin
Total Records ( 2 ) for A.N.M. Fakhruddin
  S.M. Ahsan Habib , A.N.M. Fakhruddin , Shamima Begum and Monzur Morshed Ahmed
  Optimum incubation period, temperature and pH for production of extracellular alkaline protease production by novel bacterium Halobacterium sp. AF1 were investigated. The protease were also characterized for its stability on various environmental conditions. Extracellular thermo-alkaline activity was determined by measuring proteolytic activity of the culture supernatants and stability of the protease was determined by incubating protease under different environmental conditions. The optimum production of AF1 protease was achieved within 72 h of culture at 40°C with the pH 8. The activity of the protease produced by this organism was stable up to 91% at 70°C and 83% at pH 9.5. AF1 protease was not only stable in the presence of organic solvent (25% v/v) but also exhibit a higher activity for acetone and n-hexane than in the absence of solvents. These results suggested that the protease produced by the organism was thermo-stable organic solvent tolerant extracellular alkaline protease. The remarkable stability was found for 5 mM Ca+2, Na+, Hg+2 and Fe+3 as they had been showed marginal effect on proteolytic activity of isolated protease. The AF1 protease was retained their activity up to 57-67% for 1.5% sodium dodecyl sulfate and H2O2. Higher proteolytic activity was found for gelatin (1% w/v) than that of casein or bovine serum albumin. The AF1 protease exhibited excellent stability towards temperature, pH, organic solvents and metal ions. These properties make it suitable to be used in tannery industrial applications as well as detergent additive which reduce the industrial use of chemicals wastes in the environment.
  M. Aktaruzzaman , A.N.M. Fakhruddin , M.A.Z. Chowdhury , Z. Fardous and M.K. Alam
  Accumulation of heavy metals in environmental matrices is a potential risk to living system due to their uptake by plants and subsequent introduction into the food chain. A study was conducted to investigate the heavy metals concentration in soils and leafy vegetables samples along the Dhaka Aricha Road to assess their potential ecological risk. Heavy metals concentration was analyzed by Atomic Absorption Spectroscopy. Concentrations of all the tested heavy metals except Cd in soil samples were below the permissible level. The mean concentration of Cd was found 3.99±1.85 mg kg-1. Concentrations of all the tested heavy metals except Cd and Cr in vegetables samples were lower than recommended level. Mean concentration of Cd and Cr were found 1.00 ± 0.68 mg kg-1 and 2.32±0.84 mg kg-1, respectively. Based on the Potential Ecological Risk Index, Cd posed very high risk to the local ecosystem due to its higher Risk Factor, >320 and based on Transfer Factor of Pb and Cd were found higher accumulator among the tested metals. The results of present study revealed that the bioconcentration of heavy metals along the Dhaka Aricha Road posed high risk to the ecosystem. Considering the Transfer Factor of Cd and Pb it can be suggested that plants and leafy vegetables grow in the soil near Dhaka Aricha Road should not be used as food or feed.
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