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Articles by A.N. Memon
Total Records ( 3 ) for A.N. Memon
  Shaista Khan , A.N. Memon , Allah Bux Ghanghro and Ibtessam Tahir
  Wheat is most widely cultivated cereal and important source of food and protein worldwide. Wheat is a staple diet containing most of the ingredient, for wide range of food. The unique property of wheat flour is to form dough and this property is due to gluten required for making preparation of chapatti and bread. Fractionation and characterization of seed storage proteins of fourteen wheat varieties cultivated in sindh was carried out by Sodium Dodecyle Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Wheat grains of fourteen (14) varieties were collected from different region of sindh (Pakistan). Electrogram of each variety were calculated by Jaccard’s Similarity Index (JSI). Genetic diversity of different sindh wheat varieties was measured by constructing dendogram of High Molecular Weight (HMW) and Low Molecular Weight (LMW) gluten sub unit bands. The dendrogram profile data showed the variation in the number and position of bands from one variety to the other, while some bands are common.
  Ibtessam Tahir Ansari , Mumtaz Ali Sahito , A.B. Ghanghro , A.N. Memon and Shaista Khan
  SDS-PAGE electrophoresis is the most effective as well as one of the simplest methods for separating proteins. The extraction and fractionation technique was applied to characterize rice seed proteins. Identification and screening of rice proteins carried out from 14 varieties of rice collected from Nuclear Institute of Agriculture (NIA) Tandojam. Rice seed proteins were extracted with phosphate buffer for total proteins. After quantification, proteins were separated by SDS-PAGE Electrophoresis. Analysis of rice seed proteins was performed on the results of SDS-PAGE using gel documentation system (Biorad) to find out the molecular weight of different varieties of rice followed by cluster analysis using SPSS to find out the diversity among different rice varieties.
  Fatima Ismail , Farah N. Talpur and A.N. Memon
  Fresh vegetables and fruits are considered as a healthy source of water soluble vitamins. However water soluble vitamins may lose during washing and improper storage. Environmental factors such as light and temperature also have an effect on the moisture and vitamin contents of vegetables and fruits. Vitamins are needed for normal metabolic reactions in an appropriate amount, their deficiency or increased intake can lead to different irregularities in normal cellular metabolism. Pakistan is an agrarian country with about 21.9 million hectors of cultivation, of which Sindh province offers about 28554 acres .The production of vegetables and fruits is more than 50, 300 tons annually. The consumption of fruits and vegetables is increasing on daily basis as the people are getting more aware about the nutritional importance of fruits and vegetables. Vegetables and fruits supplies from the fields of harvesting to the markets are continuously in the exposure of environmental factors for example. Light intensity, traffic pollution which directly influence the moisture contents and ultimately reduces the water soluble vitamins contents. The vitamin contents also depend on the rate of the maturity of the vegetables and fruits as the early reaping is a common practice of the local farmers to get more economical benefits. Vegetables and fruits nutrients determination on monthly basis is a common practice in developed countries but such practices are rare in developing countries like Pakistan. Therefore the objective of the current study was the determination of vitamin B complex (B1, B2, B3, B6, B9) from the selected vegetables and fruits consume frequently in the region of Sindh, Pakistan. The HPLC analysis of acid hydrolysis samples showed that thiamin (B1) was found in the range of 0.02-0.18mg/100gm, riboflavin (B2) 0.016-0.2mg/100gm, niacin (B3) 0.01-0.1mg/100gm while pyridoxine (B6) was determined as 0.06-0.28mg/100gm and folate (B9) 0.016-0.19mg/100gm which have been determined lower than the recommended ranges.
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