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Articles by A.M.G. Darwish
Total Records ( 1 ) for A.M.G. Darwish
  M.G.M. Allam , A.M.G. Darwish , E.H.E. Ayad , E.S. Shokery , R.E. Mashaly and S.M. Darwish
  Background: Enterococci represent a significant part of the microbial population in karish cheese, a traditional cheese produced in Egypt. They are Lactic Acid Bacteria (LAB) that are important in environmental, food and clinical microbiology. Objective: In vitro and in vivo evaluation of safety and probiotic traits of isolated enterococci strain targeted for functional foods preparations. Materials and Methods: Thirty three enterococci strains isolated from karish cheese were screened. The strain Enterococcus feacium (KT712) was selected according to preferable phenotypic and technological characterization then identified using 16S rRNA approach. In vitro probiotic traits were evaluated. The strain was inoculated as single culture to obtain cultured fermented milk used to feed Albino rats for in vivo evaluation. Results: The strain showed probiotic characteristics through its acid and bile salt tolerance in vitro, while, in vivo functional probiotic traits were announced in hypolipidemic effect, immunity and growth stimulation. Hematology analysis, oxidative stress parameters, carcinoembryonic antigen (CEA) levels and histological examination indicated safety of tested Enterococcus strain. Conclusion: Researchers can suggest employment of the tested Enterococcus strain as a probiotic in food or feed functional preparations innovations but more investigations such as; the presence of transferable antibiotic resistance genes can be recommended to guarantee safe use of the ingested strain. The sequence data of the isolated probiotic LAB strain, Enterococcus feacium (KT712) in this study has been deposited to the GenBank data library under the accession number KX214763.
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