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Articles by A.M.F. Al-Ansary
Total Records ( 2 ) for A.M.F. Al-Ansary
  Aida A. Rizkalla , A.M.F. Al-Ansary , S.A.A. Attia , A.A.A. Haiba and J.E. Nasseef
  Wheat anther culture produces homozygous doubled haploid plants, in a very short time span as comparing with traditional breeding methods. It is affected with several different factors, such as suitable spike stage as a donor for anthers, anthers cold pretreatment duration, rate of embryogenic callus formation and the time of anthers transferring from callus induction to regeneration medium. The effects of these factors on androgenic process were studied in this work the response of five F1 wheat hybrids, of ten varieties, differing in their salt tolerance, was investigated in anther culture system for haploid plantlets regeneration. Anthers were plated on P4S medium for callus induction, anthers were taken from different spikes ages (as in a distance between the top of the spike and flag leaf), different duration of cold pretreatment and different time of transferring anthers from callus induction to regeneration medium (190-2) were studied. Significant differences between genotypes on androgenic process were detected. Genotype influence was significant on the suitable spike length which has high percentage of proper stage of mid-uninucleate stage, consequently on both of callus induction and plant regeneration (green, albino and total plantlets). On the other hand, there was no significant influence in the embryogenic callus formation per 100 anthers between the hybrids under same duration (14, 28 and 56 days). The suitable duration for staying on induction callus medium was 28 days as the green regeneration plants increased. This is considered as accurate parameter for evaluation efficiency of androgensis for anther culture.
  H.A. Hussein , H.A. Hussein Ebtissam , O.E. El-Sayed , A.M.F. Al-Ansary , S.A. Khatab and A.A. Rabie Sally
  Grain Protein Content (GPC) of wheat is important for improved nutritional value and is also one of the major factors affecting bread making and pasta quality. This study was an attempt to improve grain protein content in local wheat cultivars by transferring the Gpc-B1 allele from wild accessions of Triticum turgidum ssp. dicoccoides to Egyptian cultivars belonging to two wheat species, i.e., Triticum aestivum (Giza 164 and Sakha 69) and Triticum durum (Beni suif 5 and Sohag 3) using interspecific hybridization. The genetic variability among the studied genotypes (5 T. turgidum ssp. dicoccoides+2 T. aestivum+2 Triticum durum) was assessed using seven agronomic characters, grain protein (GPC), iron (GFeC) and zinc (GZnC) contents. Marker assisted selection using the Xuhw 89 marker was applied to confirm the presence of the Gpc-B1 gene in the wild dicoccoides parental genotypes and the F1 hybrids. Nine out of the twelve hybrids revealed the Xuhw 89 allele of the dicoccoides (122 bp) and showed significant increase in the values of grain protein in addition to one or the two micronutrients (Fe and Zn) compared to the corresponding local cultivars. These values ranged from 88-147, 9-27 and 7-25 mg kg-1, respectively in the hybrids. While, they ranged from 72-97, 8-11 and 7-10 mg kg-1, respectively in the local cultivars. The results revealed that a weak negative correlation exists between grain yield and grain protein content. Nevertheless, some crosses (Giza 164xIrbid, RachayaxGiza 164, Sakha 69xHaifa and Sohag 3xHaifa) exhibited an increase in grain protein content without affecting the grain yield.
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