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Articles by A.M. Soliman
Total Records ( 7 ) for A.M. Soliman
  A. Bashtar , S.A. Ahmed , A.M. Soliman and M.A. Hamed
  This study has been carried out to investigate the most important aspects of metabolic integration between Schistosoma parasite and its final host under the influence of immunization with S. mansoni antigens. The possible side-effects of soluble worm antigen preparation (SWAP) and soluble egg antigens (SEA) were investigated through estimation of succinate dehydrogenase, lactate dehydrogenase, glucose-6-phosphatase, acid phosphatase and 5`-nucleotidase enzymes activities in hepatocytes of mice. Further, liver function enzymes as aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase were estimated. Cells viability, no of worm burden and ova count were also investigated. The study has revealed that immunization with S. mansoni antigens protect against schistosomiasis by different degree of protection without any side-effects lead to improvement of liver enzymes under study.
  A.M. Soliman , B.N. Barsoum , G.G. Mohamed , A.A. Rezk , A.E. Aboul-Ata and H.M. Mazyad
  This study, aims at determination of efficiency of micro interfering RNA (miRNA) to develop ability of virus resistance against Egyptian PVX isolate (PVX-Eg2) in both potato (Solanum tuberosum L. cv. Spunta) and tobacco (Nicotiana benthamiana). RNA constructs of Sense (PVX-Eg2cpVs), antisense (PVX-Eg2cpCs) and sense/antisense were designed, cloned and sub- cloned for gene transfection using Agrobacterium inoculation technique. Two to 3 leaf-stage seedlings of potato (Solanum tuberosum L. cv. Spunta) and tobacco (Nicotiana benthamiana) were inoculated with the three previous constructs. The construct-treated plants were mechanically inoculated with the PVX-Eg2 isolate. Bioassay and PCR amplification have been able to evaluate transfected-plant resistance against PVX-Eg2 that is caused by siRNA of PVX-Eg2cp. PCR amplification has been able to detect PVX viral genome in all challenged plants those infiltrated with either pFGC5491 vector without insert, or with sense construct and also with antisense construct. Bioassay has confirmed same previous statement. Nine out of 10 sense/antisense-transfected potato plants were negatively reacted with both bioassay and PCR amplification. Same negative reaction has been viewed using both bioassay and PCR for sense/antisense transfected-tobacco plants. Seven out of 10 proved they are PVX-Eg2 resistant.
  W.S. El-Araby , I.A. Ibrahim , A.A. Hemeida , Amal Mahmoud , A.M. Soliman , A.K. El-Attar and H.M. Mazyad
  Viruses are major diseases affecting potato plantations in Egypt. Different molecular and serological methods for virus detection were used to detect the Potato virus X (PVX), Potato virus Y (PVY) and Potato leaf roll virus (PLRV). ELISA, RT-PCR, immunocapture RT-PCR (IC-RT-PCR) and RT-PCR-ELISA were the detection methods used. Naturally virus-like infected potato samples were collected from different locations at El-Munofia Governorate in Egypt. PVX and PVY were isolated and identified biologically through host range and indicator plants. All detection methods mentioned above were sensitive enough to detect the three potato viruses in potato tissues. Using ELISA method, PVX, PVY and PLRV were detected in 4, 7 and 10 out of 26 samples (15.4, 26.9 and 38.5%), respectively. Mixed infection was found between PVX/PVY; PVY/PLRV and also among PVX/PVY/PLRV. According to the results, it is the use of molecular methods to confirm certain serological results i.e., the virus especially when low concentration in the infected samples. Using the advantage of IC with RT-PCR-ELISA will reduce the costs and avoid RNA degradation. Additionally, RT-PCR-ELISA could be the method of choice among the molecular methods.
  H.S. El-Helaly , A.A. Ahmed , M.A. Awad and A.M. Soliman
  Alfalfa Mosaic Virus (AMV) was isolated from naturally infected potato (Solanum tuberosum, L. cv. Spunta) plants, showing bright yellow blotching or mottling Calico and aucuba symptoms suspected to be due to virus infection. Leaf samples were collected from different localities in Minufyia Governorate, Egypt. AMV was readily mechanically inoculated by sap extracted from infected potato leaves to host plants (bioassay). The identity of AMV isolate was confirmed by sequence analysis of their Coat Protein (CP) gene. The sequence of the Coat Protein gene (CP) of AMV was determined from cDNA clones. The CP gene was cloned into pGE®-T Easy vector and transformed into Escherichia coli strain DH5α. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank. The analysis showed that nucleotide sequence of the Egyptian isolate of AMV (GenBank Accession No. HQ288892) had percentage of similarity ranged from 97-98% with eight reported AMV isolates. Thus, a method of identification and detection by RT-PCR of AMV was established.
  A.R. Sofy , A.A. Mousa , A.M. Soliman and Kh.A. El- Dougdoug
  Gummy bark disease is a disorder of sweet orange on sour orange rootstock in Egypt. There is an importance for hot-growing temperatures to symptom development of citrus viroids. The geographical distribution of the gummy bark disease in some world countries depend on high temperatures for viroid-symptom expression. So, correlation between climatic factors and gummy bark disease through GIS is studied. We designed a satellite map for the gummy bark disease distribution all over the world using the previous registered results. Superimposed maps of BIOCLIM annual Min-temperature, Max-Temperature and the points distribution, indicated that gummy bark disease naturally occurs in the low temperature zones range from 8-18°C at winter and from 27-38°C at summer season where the altitude ranged from -351 to 1320 m. A novel method called maximum entropy distribution modeling was used for predicting potential suitable habitat for gummy bark disease in Egypt using occurrence records. The Maxent model’s internal jackknife test of variable importance showed that altitude and mean temperature of driest quarter are the most important predictors of citrus gummy bark disease-habitat distribution.
  A.R. Sofy , A.M. Soliman , A.A. Mousa and Kh.A. El-Dougdoug
  This study reports the characterization of Hop stunt viroid which has been isolated previously in Egypt from sweet orange infected with gummy bark disease namely citrus viroid II. The native structure of mature, circular forms of the gummy bark agent was detected by gel electrophoresis. The expected size of amplified cDNA by RT-PCR was approximately 300 bp. A phylogenetic tree of the Egyptian citrus gummy bark agent (Accession no. FJ984562) revealed 100, 99.7 and 97.8% a moderate degree of similarity to CVd-IIb (USA), CCaVd (Spain) and CCaVd (Egypt), respectively. The minimum free energy of a secondary structure for HSVd-EG-RNA was determined using its primary sequence at 37°C. The sequence appeared to fold into a rod-like structure at -122.1 kcal moL-1 while CCaVd-EG at -120.5 kcal moL-1. The five domains of the rod-like structure were determined. The sequence variations between Egyptian citrus gummy bark isolate and Egyptian citrus cachexia isolate in the pathogenic domain (P) tend to influence the pathogenicity of the HSVd-EG. Finally, the genetic diversity and evaluation of entropy power for the Egyptian citrus gummy bark agent and HSVd-citrus populations registered in GenBank, were viewed against the phylogenetic background of known CVd-II variants including the non-cachexia (CVd-IIa) and the causal agents of severe (CVd-IIb, CVd-IIc), more moderate (Ca903) and mild (Ca909).
  M.S. Al-Saikhan , K.A. Alhudaib and A.M. Soliman
  Potato Leaf Roll Virus (PLRV), Potato Virus Y (PVY) and Potato Virus X (PVX) are the most economically important viruses in commercial potato production. Enzyme Linked Immunosorbent Assay (ELISA) and the Reverse Transcription Polymerase Chain Reaction (RTPCR) were used for the detection of these three potato viruses. Specific primer pairs designed to amplify the coat protein gene of each virus (627 bp for PLRV, 801 bp for PVY and 714 bp for PVX) were successfully applied. A multiplex RT-PCR (mRT-PCR) was developed for the simultaneous detection of the three viruses in potato leaves. The nucleotide sequences of the coat protein genes of the Saudi isolate of PLRV (PLRV SA3), the Saudi isolate of PVY (PVY SA2) and the Saudi isolate of PVX (PVX SA1) were submitted in the GenBank under accession numbers: KC875235, KC875237 and KC875236, respectively. The nucleotide sequences PLRV SA3, PVY SA2 and PVX SA1 were compared to the sequences of the coat protein genes of other PLRV, PVY and PVX isolates. The similarity of the nucleotide sequences suggested that the architecture of the polerovirus (PLRV), potyvirus (PVY) and potexvirus (PVX) are highly conserved. This study describes an assay where three common potato-infecting viruses, Potato leafroll virus, Potato virus Y and Potato virus X, were detected simultaneously from total RNA potato leaves in a multiplex RT-PCR.
 
 
 
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