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| Articles
by
A.M. Saadabi |
Total Records (
5 ) for
A.M. Saadabi |
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Ehssan H.O. Moglad
,
Omer M. Abdalla
,
W.S. Koko
and
A.M. Saadabi
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In this study, eighty percent methanol and chloroform extracts of leaves and stems of Solanum nigrum were screened for in vitro anticancer activity using PC-3 human prostate cancer cell lines and Hela cervical cancer cells and cytotoxicity assays were performed using MTT assay and 3T3 NIH mouse embryo fibroblast cell line and CC-1, a rat Wistar hepatocyte cell line. All extracts in concentration 100 μg mL-1 showed anticancer activity in PC-3 and Hela and the highest percentage of growth inhibition obtained from stems methanol extracts on Hela 91.11% followed by leaves and stems methanol extracts (74.28 and 80.49, respectively) on PC-3. For cytotoxicity the result obtained indicate that all extracts had non-toxic effect on CC-I and 3T3 cell lines with IC50> 100 μg mL-1, except the leaves methanol extract showed the highest percentage of growth inhibition in 3T3 85.63 with IC50 17.37 μg mL-1. The results obtained indicate that Solanum nigrum leaves and stems methanol extracts have anticancer activity on prostate cancer, cervical cancer and have non-toxic effect on 3T3 and CC-1. This result supports the traditional use of Solanum nigrum for the treatment of cancer in different regions of the Sudan. |
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A.M. Saadabi
and
Ansam H. Mohammed
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Methicillin Resistant Staphylococcus Aureus (MRSA)
bacteria is one of the famous widely distributed in the world and source of
multiple diseases like cellulites and endocardities in all age groups. In the
present study, fifty samples were taken as nasal swabs at time of admission
to the hospital. About 44% were positive for Staphylococcus aureus (MRSA)
and 0% were Methicillin-Sensitive Staphylococcus aureus (MSSA). MRSA
colonization in males was 10% while in females were 12% which indicate that
nasal colonization of MRSA in females is higher than males. MRSA nasal colonization
among patients within 0-15 years old was 2%, patients within 16-30 years old
was 6%, patients within 31-45 years old was 11%, patients within 46-60 years
old was 2% and patients >70 years old was 1%. Sensitivity of the all staphylococcus
aureus isolated samples to different antibiotics was studied using kanamycin,
erythromycin, methicillin and vancomycin. It was found that all of the S.
aureus strains were resistant to methicillin antibiotic (100%) and the rate
of sensitivity to other drugs was72% for kanamycin, 9% for erythromycin and
22% for vancomycin. This study confirm that kanamycin is a drug of choice so
far for treatment of MRSA although, there is slight resistance. |
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Ehssan H.O. Moglad
,
Muddathir S. Alhassan
,
W.S. Koko
and
A.M. Saadabi
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The development of drug resistance as well as appearance of undesirable side
effects of certain antibiotics has lead to the search for new antimicrobial
agents mainly from plant extracts with the goal to discover new chemical structures.
To overcome these disadvantages, the research was carried out to investigate
the in-vitro antibacterial and antifungal activity of Cadaba farinose
(Capparaceae), Solanum nigrum (Solanaceae), Senna occidentalis
(Caesalpiniaceae) and Maerua oblongifolia (Capparaceae). Eighty percent
Methanol and chloroform extracts of leaves of plants were screened for their
antimicrobial activity against different pathogenic bacteria and fungi. These
were Staphylococcus aureus, Bacillus subtilus, Escherichia
coli, Salmonella typhi and Aspergillus niger and Candida
albicans using the cup plate agar diffusion method. All methanol extracts
exhibited inhibitory effects against all tested organisms with zones of inhibition
ranging from 11-25 mm except the methanol extract of both parts of Maerua
oblongifolia were active against Escherichia coli and Aspergillus
niger. The results obtained from plants extracts were compared to some of
the commercially used drugs. The Minimum Inhibitory Concentrations (MICs) of
the most active extracts of these plants against standard bacteria and fungi
were also determined and found that MICs a ranging between concentration 2.5-5
mg mL-1. All of the plants extracts were phytochemically screened
and triterpenes, saponins and tannins were present in all of the methanolic
extracts. Coumarins, sterols and triterpenes were found in all choloroform extracts,
this finding indicated that these extracts of such plants promising antimicrobial
agents. |
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Mohammed S. Alhussaini
,
A.M. Saadabi
,
Mohammed I. Alghonaim
and
Khalid Elfakki Ibrahim
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The present work was aimed to evaluate oleo-gum resins aqueous, methanol and chloroform extracts of Commiphora myrrha for antimicrobial activity against four types of bacteria and twelve species and strains of fungi. All of the oleo-gum resin extracts irrespective of their types inhibited the growth of all microbes to varying degrees. Aqueous extract showed the least antibacterial and antifungal activity against all of the pathogens used especially with regard to gram positive bacteria Staphylococcus aureus (11 mm) and Escherichia coli (11 mm) in concentration of 100 mg mL–1 as compared to methanol or chloroform. Less or no activity was observed against Trichophyton concentricum (7.01 mm) and 4.01 mm inhibition zone for Candida rugosa in the same concentration used. The oleo-gum resins methanol extracts in different concentrations were significantly inhibitory to the growth of the different tested fungal dermatophytes. Reduction in mycelial weight of fungi was directly correlated with concentration of extract. The concentration of 200 mg mL–1 of myrrha was the most inhibitory against Epidermophyton floccosum with 2 g mycelial fresh weight while the less inhibition in the growth was obtained in Candida albicans and it was 6.61 g in the same extract concentration. The other used fungal or bacterial groups showed varying degrees of activity pending on the type of extract used. The Minimum Inhibitory Concentration (MIC) values of methanol and aqueous extracts of oleo-gum resins of myrrh showed that the highest values were obtained in methanol extract for Trichophyton concentricum and lowest MIC values for the same extract for the bacterium Staphylococcus aureus. In the aqueous extract of myrrha the highest and lowest MIC values were found for Bacillus subtilis and Pseudomonas aeruginosa. Phytochemical analyses showed the presence of Sesquiterpenes (Isoprenoids i.e., Terpenoids) and Furanosesquiterpenes as major constituents of the oleo-gum resins of the plant. These results confirm the antibacterial and antifungal activity of gum resins and support the traditional use of the myrrh in therapy of bacterial infections. |
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Mohammed S. Alhussaini
,
A.M. Saadabi
,
Kamal Hashim
and
Abdullah A. Al- Ghanayem
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The aim of the present study was to apply natural drops of truffle (Terfezia claveryi) to patients diagnosed with trachoma for curing the disease and inhibiting the chlamydial bacteria and explore the potential for the development of new treatment modalities compared to currently used antibiotics. Results showed that the truffle juice was very effective against trachoma in the third phase of the disease. There was clear reduction of symptoms in patients with cortisone compounds in the form of drops plus truffle juice drops. There were also complete healing of the conjunctiva and healing of the keratitis. Partial curing was observed when cortisone plus cream of chloramphenicol was added included healing of trachoma without any change of fibrosis in the conjunctiva. In the treatment of trachoma with cortisone drops and cream plus truffle juice, complete of healing was observed but continued swelling in the capillaries with no fibrosis occurred. It was clearly noticed that in all of the treatments, there was a noticeable reduction in fibrosis of the affected site in the eye. It was also concluded that truffle juice seems to prevent fibrosis of trachoma by a large extent. On the other hand, the use of chemical antibiotic or cortisone-based compounds produced several side effects. Though these chemicals reduced the toxins caused by trachoma thereby reducing the enlargement of the cells. We can therefore clearly recommend adding truffle juice to the traditional treatment for trachoma at all stages. |
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