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Addition of nitrogen sources as supplementary nutrient
into MSM medium to enhance biodegradation by stimulating the growth four
isolates, Acinetobacter faecalis, Staphylococcus sp., Pseudomonas
putida and Neisseria elongata isolated from petroleum contaminated
groundwater, wastewater aeration pond and biopond at the oil refinery
Terengganu Malaysia was investigated. The organic nitrogen sources tested
not only supported growth but also enhances biodegradation of 1% Tapis
crude oil. All four isolates showed good growth especially when peptone
was employed as the organic nitrogen compared to growth in the basal medium.
Gas chromatography showed that more then 91, 93, 94 and 95% degradation
of total hydrocarbon was observed after 5 days of incubation by isolates
Pseudomonas putida, Neisseria elongate, Acinetobacter
faecalis and Staphylococcus sp., respectively.
