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Articles by A.M. Hanafy
Total Records ( 3 ) for A.M. Hanafy
  A.M. Hanafy , H.A. Khalil , Omnia E. Kilany , Marwa A. Hassan , Mohamed S. Yusuf , Abdelazim Ibrahim , I.M. Fares , A.M. Hassan and P.G. Reddy
  The objective of this study was to determine the optimal level of an Oil Mixture (OM) supplementation in drinking water to enhance the performance of older Japanese quail. Five hundred forty Japanese quail, 40 weeks old, were randomly assigned to 4 experimental groups (90 female and 45 male/group) that received OM at 0, 0.5, 1 and 2 mL/L–1 of drinking water during the experimental period of 42 days. Egg production, egg quality, fertility and hatchability percentages were evaluated. Serum alanine aminotransferase (ALT), aspartate transferase (AST), total protein, albumin, urea, creatinine, total lipids, cholesterol and triglycerides were estimated. Livers were examined for histopathological changes. Results showed that birds received 0.5 mL/L–1 of OM had significantly improved in most studied traits compared to the other treated and control groups. Laying rate, fertility, hatchability percentage, yolk index, internal quality unit and ovarian yellow follicle number were superior in 0.5 group than the other experimental groups. In contrast, birds received 1 or 2 mL had significantly higher concentrations of ALT and AST than birds that received 0.5 mL or control birds. Serum chemistry analysis revealed no significant effects due to treatments on kidney functions. Histopathological findings revealed disruption of normal hepatic architecture in birds that received 1 or 2 mL of OM supplementation compared to 0.5 mL and control birds. Our findings suggest that 0.5 mL/L–1 of OM could be enough and useful in improving productive and physiological performance of laying Japanese quail.
  A.M. Hanafy and H.A. Khalil
  The present study was conducted to determine the effects of chronic Dexamethasone (DEX) administration on physiological traits and semen characteristics in male Japanese quail breeders. Forty five 20 week old male quail were distributed into 3 experimental groups. All groups were fed on the same basal diet and administered with different levels of DEX, at 0 (as a control group), 0.25 and 0.50 mg/bird/day orally for 14 consecutive days. Body weight, feed intake, cloacal gland area and fertility percentage were recorded. Also, male genital organs were investigated and semen characteristics were estimated at the end and after 28 days of administration ended. Serum testosterone and corticosterone levels were analyzed by ELISA. The results revealed that, there were no significant effects of ingested DEX at different levels on body weight, feed intake and testes weight of the male Japanese quail breeders. However, the administration of DEX at 0.25 and 0.50 mg/bird decreased significantly cloacal gland area, foam production, sperm motility and viability and testosterone level. Also, treatments with DEX increased Time of Sexual Libido (TSL), Methylene Blue Reaction Time (MBRT) and serum corticosterone level compared with control group. Also, significant decrease in fertility percentage was observed after male exposure to DEX. Even after 4 weeks from administration ended, libido and sperm motility were significantly impaired. Also, significant negative correlations were found between serum corticosterone level and most studied traits. Our results indicated that chronic DEX administration had markedly effects on the reproductive performance of male quail for several weeks even after exposure to a stressors ends.
  A.M. Hanafy , H.A. Khalil , W.M. Abdel-Rahim and A.M. Abdel-Ghany
  Recently, with the world progress of industries, several adverse factors such as Endocrine Disrupting Chemicals (EDCs) have appeared. These contaminants have negative devastating effects on the reproductive performance in a large number of domestic and wildlife animal species. Therefore, the objective of this study was to investigate the reproductive and physiological changes due to pre-pubertal exposure of Japanese quail males to Bisphenol A (BPA) as one of EDCs (BPA is a synthetic chemical acts as estrogenic effect). Three weeks old male quails were weekly injected intra-peritoneally (at 3, 4 and 5 weeks old) with BPA at doses of 0, 1, 5, or 10 mg kg–1 b.wt. After 6 weeks exposure of BPA, time of sexual libido, semen characteristics, fertility percent, sexual organs development, histopathology of testes were examined and plasma testosterone concentrations were estimated. The results showed that BPA has adverse and deterioration effects on most of the studied traits. The males received 5 and 10 mg BPA showed significantly delayed time of sexual libido compared with control group. Also, males received 5 mg BPA showed significantly reduced semen volume and cloacal gland area compared with control males. The lowest initial motility and fertility percent (p≤0.05) were detected in 10 mg group while the highest values were obtained in the control group. Males treated with 1 and 10 mg BPA had lower (p = 0.028) foam production than those produced in control males. Plasma concentrations of testosterone were significantly reduced (p<0.000) in all treated groups compared with control group. Histologically, the growth of the testes was negatively affected by exposure to/or over 1 mg kg–1 BPA: Namely, the development of seminiferous tubules and spermatogenesis were severely inhibited compared with control testes. It could be concluded that exposure to estrogenic effects of environmental endocrine disruptors such as BPA before/at puberty lead to malformation of reproductive organs and reduction of reproductive capacity which appears not to regenerate in adult male quail.
 
 
 
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