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Articles by A.M. Abdel-Salam
Total Records ( 9 ) for A.M. Abdel-Salam
  A.I. Al-Humaid , H.M. Mousa , R.A. El-Mergawi and A.M. Abdel-Salam
  Dates and camel milk have been reported to be used for prevention and treatment of various diseases in Arab countries. This study was designed to investigate the antioxidant activity of dates and camel milk, alone or as a mixture and their effect in lead acetate-poisoned rats. Lead was administered to rats in drinking water at a concentration of 0.6% to generate lipid peroxidation. Three cultivars of date fruits grown locally (Nabtat Ali, Sukkari and Rashudia) were tried when mixed individually with camel milk against lipid peroxidation generated by lead. Chemical composition of dates, camel milk and their mixture was determined. The organoleptic evaluation of palatability of date-camel milk mixtures was performed. Serum glucose, triglycerides and urea concentrations and activities of Aspartate Aminotransferase (AST/GOT), Alanine Aminotransferase (ALT/GPT) and Glutathione-S-Transferase (GST) enzymes were determined. Drinking water containing 0.6% lead acetate resulted in significant increase in serum glucose, triglycerides and urea concentrations, as well as increased activity of serum enzymes were investigated. It also reduced the serum activity of the antioxidant enzyme Glutathione-S-transferase. Mixing camel milk with date varieties of Rashudia, Nabtat Ali or Sukkari in ratio of 2:1 (v/w) increased milk antioxidant capacity by 49.2, 62.8 and 81.3%, respectively. It also reduced the elevated activities of serum enzymes, the concentration of urea and increased the activity of Glutathione-S-Transferase enzyme in poisoned rats. Mixtures of camel milk with the different cultivars of dates produced different beneficial effects. In conclusion, feeding a mixture of camel milk with dates seems to enhance the body defense against free radicals generated by the lead acetate poisoning mainly due to the high contents of phenolics and flavonoids in the mixture.
  A.M. Abdel-Salam
  Functional foods will be hopeful to good health in the future because it have been classified either as preventive or therapeutic purpose and used alone or mixed together for prevention some of certain diseases. Therefore, functional foods help the rights of protection from many modern diseases that we see today. However, increasing and varieties of the functional foods are a challenging perspective for promotion and formulation of healthy food and which can be useful for enhancements of the human health.
  K.A. Al-Sobayil , M.M. Zeitoun , M.H. Khalil and A.M. Abdel-Salam
  The present study aimed at investigating the biological evaluation of a synbiotic fermented milk synergistic with some active ingredients of herbal hydrosols and honey on the sexual activity, semen characteristics and testosterone levels in Aradhi and Damascus goat`s bucks. A synbiotic syrup was prepared by mixing fermented cow`s milk containing some probiotic strains and natural antioxidants isolated from functional food and herbal hydrosols. Fifteen Damascus and Aradhi bucks were utilized in the experiment. Two bucks served as control (given no synbiotic), 7 bucks were designed to be orally given twenty ml of the mixture three times per week for 8 consecutive weeks (Low dose, Aradhi-Low = AL, Damascus-Low = DL) and 6 bucks were orally given 40 mL of the mixture (High dose, Aradhi-High = AH, Damascus-High = DH) by the same regime. Bucks were tested for the libido and semen ejaculates were collected once a week for testing the physical characteristics. A jugular blood sample was taken once a week in a non-heparinzed tube for the determinations of serum testosterone, alanine and aspartate amino transaminases (ALT and AST), urea and creatinine levels. Results indicated a significant enhancement of the libido due to the breed of the buck; Aradhi bucks exhibited less reaction time than Damascus bucks (p<0.01). The high dose of the mixture enhanced (p<0.01) the libido in both breeds. Testosterone levels were significantly (p<0.01) higher in Aradhi than Damascus bucks. There found significant increase in testosterone concentrations in treated Aradhi than in Damascus bucks. There were no differences between low and high mixture dose. Administration of the mixture resulted in an increased (p<0.01) ejaculate volume, gross and individual motility, sperm concentration, total motile sperm in the ejaculate than in control. Additionally, lower dead and abnormal sperm numbers were obtained with bucks given the mixture. Treatment slightly increased AST and urea but significantly increased ALT and creatinine in both breeds. In conclusion, giving goat bucks a mixture of a synbiotic functional syrup enhanced the metabolic activity resulting in improvements in their reproductive performance.
  M.M. Abdelsalam , M.M. Zeitoun , M.A. Ateah , A. Al-Hassan and A.M. Abdel-Salam
  As pregnant ewes require more nutrients during their late stage of pregnancy, the current study aimed at investigating the supplementation of late pregnant Najdi ewes with a probiotic fermented milk, sukkary date fruit extract and their mixture on the neonatal traits and maternal hematological parameters. Twenty mature late pregnant Najdi ewes were randomly allocated into four treatment groups (n = 5 group-1). Group 1 (C) ewes served as control and given normal saline in a similar program as treated ewes, group 2 (D) ewes were administered 50 mL date extract every other day for the last eight weeks of pregnancy, group 3 (M) ewes were given 50 mL of probiotic fermented cow's milk for the same period and group 4 (DM) ewes were given 50 mL of a mixture (date extract: fermented milk, 1:1) for same period. A blood sample was collected via the jugular venipuncture at the commencement of the experiment and thereafter once a week for the first four consecutive weeks of the treatment. Data were collected after lambing for the neonatal traits. Whole blood samples were analyzed for hematological parameters. Results exhibit no variation for maternal lambing weight among treatments. Mean litter size was not different among treatments (1.2, 1.2, 1.4 and 1.4 lambs/ewe for C, D, M and DM, respectively). Percent lamb survival at birth was highest in D (100%) and lowest in C (67%) ewes with values of 85.7 and 71.4% in M and DM ewes, respectively. Mean litter weight at birth increased (p<0.01) in D (6.50±0.3 kg) than control and other treatments. This increase comprised about 85% over the control litter weight. A slight increase (p>0.05) in litter weight was found in DM ewes (4.0±1.5 kg), whereas ewes given fermented milk alone gave birth of a litter weight comparable to that of control. Date extract significantly (p<0.01) increased hemoglobin concentration (10.74±0.2 g dL-1) as compared to control (9.53±0.2 g dL-1). Fermented milk resulted in the highest White Blood Cell count (11.12±0.5x103 μL-1) and the lowest Red Blood Cell count (7.84±0.4x106 μL-1). All treatments tended to decrease the percentage of segmented neutrophils, meanwhile treatments tended to increase the percentage of lymphocytes with similar percentages of other types of leukocytes. In conclusion supplementing pregnant ewes with dates extract might represent an available source of simple sugars that provide the fetus/fetuses with energy requirements.
  E.M. El-Sayed , A.M. Abdel-Salam , S. Badran and G.A. Ibrahim
  The present research aimed to study the effect of chicory extract and lactulose syrup on the growth and viability of three probiotic organisms (Lactobacillus plantarum, Lactobacillus casei and Lactobacillus rhamnosuss) and regular yoghurt cultures (Streptococcus subsp. thermophilus, Lactobacillus delbrueckii subsp. bulgaricus) in skim milk. Three concentrations of probiotic inoculums and prebiotic ingredients (0.5, 1 and 1.5% for probiotic and 1, 3 and 5% for prebiotic) were used to select the suitable ratio in the application experiments. The tested cultures with or without prebiotic were incubated on 42 and 37 °C. The obtained results showed that the growth of Lactobacillus plantarum, Lactobacillus casei, Lactobacillus rhamnosuss and yoghurt cultures (Streptococcus thermophilus, Lactobacillus bulgaricus) were affected by the incubation temperature in all of the tested bacteria and inoculation percentage in all of probiotic strains individually or mixed with traditional yoghurt cultures. The percentage of viability in traditional yoghurt cultures were increased when cultures were incubated at 42°C compared with the cultures incubated at 37°C and different from the viability of Lactobacillus plantarum, Lactobacillus casei and Lactobacillus rhamnosuss which, were increased with incubation at 37°C compared with incubation at 42°C. Also, data found that the generation time of all probiotic cultures was decreased in the presence of chicory extract and lactulose syrup due to the enhancement of growth in probiotic cultures. The obtained results can help our further studies for selection the optimum inoculation percentage and incubation temperatures of tested strains individually and/or mixed with traditional yoghurt cultures in the application experiments.
  A.M. Abdel-Salam , Naglaa Ammar and A.Z. Abdel-Hamid
  The aim of the present study is to investigate the preparation and biological evaluation of a probiotic labneh synergistic with garlic and onion oil against Schistosoma mansoni in infected mice. The results pointed out that, probiotic labneh containing garlic and onion oil have demonstrated an anti-schistosomal activity against Schistosoma mansoni-infected mice. The levels of protection reduction ratio in total worm couple, male and female ratio were 66.67, 57.14 and 50.0% in mice infected with Schistosoma mansoni fed on probiotic labneh with garlic oil and were 50, 57.14 and 50% with onion oil, respectively. The mean percentage of reduction in total ova count in liver were 70.0 and 56.44% in mice infected with S. mansoni and fed on probiotic labneh containing garlic and onion oil, respectively. The mean values of immature, mature and dead oogram in mice infected with S. mansoni and fed on probiotic labneh containing garlic oil were 44.0±1.47, 43.0±3.5 and 7.0±2.5, respectively and 47.0±2.82, 46.0±0.77 and 5.0±0.57 in mice infected with S. mansoni and fed on probiotic labneh containing onion oil respectively. Data, also, showed that probiotic labneh containing garlic and onion oil increased the dead oogram in mice infected with S. mansoni. Data can be concluded that probiotic labneh containing garlic and onion oil may be play a great role as a protective food to control parasites.
  N.A. Al-Wabel , H.M. Mousa , O.H. Omer and A.M. Abdel-Salam
  The aim of the present study was to investigate the biological effects of aqueous herbal extracts mixed with stirred yoghurt filtrate against alloxan-induced oxidative stress and diabetes in rats. Aqueous extracts of six medicinal plants: fenugreek, greater burdock, goat`s rue, colocynth, chicory and lupine were mixed with stirred yoghurt filtrate and used in the experiments. Blood glucose and alanine and aspartate aminotransferase (ALT and AST) activities were estimated before and after alloxan-induced oxidative stress and diabetes in rats. Obtained results showed that blood glucose levels in sera of treated rats fed on aqueous extract of medicinal plants and stirred yoghurt filtrate mixture decreased with mean values of 135.0 ± 26.85 mg/100 mL serum compared with the treated rat fed on basal diet (positive control) with mean value of 237.66 ± 14.43 mg/100 mL serum. Data showed that ALT and AST activities in sera of treated rat fed on aqueous extract of medicinal plants and stirred yoghurt filtrate mixture were nearest to the level of un-treated rats fed basal diet (negative control). The means values of ALT and AST level in treated group fed on aqueous extract of medicinal plants and stirred yoghurt filtrate mixture were 57.33 ± 20 and 189.33 ± 48.85 compared with the positive control 90 ± 31.76 and 260.00 ± 57.27 and negative control 44.66 ± 9.5 and 180.66 ± 23.58 U L-1, respectively. Data concluded that mixture of medicinal plant extracts and stirred yoghurt filtrate may play a role in protection against alloxan-induced oxidative stress and diabetes in rat.
  A.M. Abdel-Salam , A.K. El-Attar and C.F. Gambley
  A polyclonal antiserum to a recombinant Coat Protein (CP) of Potato virus Y (PVY) was developed and its effectiveness was measured with double antibody sandwich immunosorbent assay (DAS-ELISA), indirect ELISA (I-ELISA), Indirect Plate Trapped Antigen (IPTA) ELISA, Western Blotting (WB) and dot blotting immuno binding assay (DBIA). The CP gene of PVY was amplified with the Reverse Transcription-Polymerase Chain Reaction (RT-PCR) using primers, designed from a recombinant CP sequence for PVYO (common strain) and PVYN (the necrotic strain), to amplify the full CP from a mixture of different PVY isolates (PVYO, PVYN, PVYNTN and PVYN:O). The full 800 bp-CP amplicon gene was cloned and expressed into pBAD-C terminal 6xHis Tag TOPO expression vector in Escherichia coli BL21. The CP fraction from bacterial lysates was purified, under native and denatured conditions, by nickel-nitrilotriacetic acid (Ni-NTA) batch chromatography; yielding 0.6 mg mL-1. Antigenicity of the purified CP fraction was measured with Western Blotting (WB) analysis. For immunization, the CP fusion protein was injected into rabbits. The recombinant PVY-CP antiserum reacted with PVY-infected potatoes using IPTA-ELISA and DBIA and with a wide spectrum of local and foreign strains of PVY including PVYO, PVYN and PVYNTN in DAS-ELISA and DBIA. The data indicated that the produced recombinant antiserum was efficient and accurate in determination of negative and positive results in ELISA tests. Therefore, this antiserum is suitable for certification programs of potatoes due to its low cost, high specificity, feasibility and its endless supply from recombinant bacterial clones carrying the CP genes for this virus.
  A.M. Abdel-Salam , M.M. Zeitoun and M.M. Abdelsalam
  This study aimed to evaluate the growth performance, blood metabolites, insulin and testosterone and wool traits of growing lambs fed with a diet containing low and high level of a synbiotic. A total of 14 growing Najdi male lambs (aged six months and weighed 22.02±0.41 kg) were randomly divided into three groups; control group (n = 4) fed a basal diet (C), low level group (n = 5) fed basal diet plus 20 ml of synbiotic (L) and the high level group (n=5) fed basal diet plus 40 ml of synbiotic (H). Synbiotic was given orally for a period of 12 consecutive weeks. Animals were bled for serum once a week throughout the experiment. Serum total protein, albumin, glucose, cholesterol, insulin and testosterone were quantified. Wool traits were also studied. Growth rate was determined of the biweekly routine body weights. Lambs given high level of synbiotic showed the highest growth (p<0.01) gain (13.74 kg) and had the heaviest weight (35.76 kg) followed by lambs given low level (8.75 and 30.87 kg, respectively) and then by the control lambs (7.33 and 29.36 kg, respectively). Total protein concentration was significantly higher in H-lambs compared with C-lambs (7.93 vs. 6.43 g dL-1, respectively) with intermediate level in L-lambs (6.72 g dL-1). Blood cholesterol decreased (p<0.05) in L-lambs (33.67 mg dL-1) compared with C-lambs (49.32 mg dL-1) whereas H-lambs gave a value of 44.18 mg dL-1 in blood of lambs with high level of synbiotic. Glucose in blood was not different (p<0.05) due to either L (58.47 mg dL-1) or H (66.14 mg dL-1) synbiotic compared with C (61.43 mg dL-1) lambs, even though there found a significant (p<0.05) difference between L and H on glucose concentration. Insulin levels significantly (p<0.01) decreased at conclusion of the experiment as compared with initial levels. The highest (p<0.01) level of insulin was found in H-lambs (27.3 μIU mL-1), however low level of synbiotic was not differ than control. Testosterone increased (p<0.05) by about 52% in H-lambs (5.0 ng mL-1) than in control (3.3 ng mL-1) lambs. The high level of synbiotic exhibited the shortest staple, lowest crimp and intermediate diameter of wool fibre.
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