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Articles by A.K.M.N. Huda
Total Records ( 4 ) for A.K.M.N. Huda
  A.H. Kabir , M.A. Bari , A.K.M.N. Huda , M.A. Rezvy and I. Mahfuz
  The investigation was based on direct shoot formation from shoot tip explant, subsequent morphogenesis and rooting of the in vitro proliferated shoots and transplantation of regenerated plants under ex vitro condition. Through proper growth regulators, it was possible to differentiate multiple shoots from shoot tip of papaya (Shahi). In the present investigation, it was observed that shoot proliferation was best in MS medium containing BAP 1.0 mg L-1+KIN 0.5 mg L-1 and BAP 1.0 mg L-1+NAA 0.5 mg L-1. Maximum number of shoot per culture (28.2) and length of the largest shoot (1.7 cm) was found on MS medium supplemented with BAP 1.0 mg L-1+KIN 0.5 mg L-1. Shoot proliferation efficiency was found the best in 11 weeks old explant in MS medium containing BAP 1.0 mg L-1+KIN 0.5 mg L-1. Sucrose in 30 g L-1 concentration as carbon source was proved to be best for shoot proliferation (82%). Maximum number of shoot per culture (41.2) and maximum length of shoot were obtained in the aforesaid concentration of carbon source. For adventitious rooting of regenerated shoot, MS medium containing IBA 1.0 mg L-1+NAA 0.5 mg L-1 was found to be the best. In this growth regulator, treatment 92% of rooting was observed. Longest length of the root (7.9 ± 0.121 cm) was found on MS medium supplemented with IBA 1.0 mg L-1+IAA 0.5 mg L-1. After transplanting the 20 days old rooted shoots into garden soil, compost and sand (2:1:1), 80% of survivability after 5 weeks was achieved.
  A.K.M.N. Huda and M.A. Bari
  This study describes the investigation on the technology of synthetic seed production using somatic embryos in two varieties (Loda and China) of eggplant. To standardize a media composition of artificial endosperm of synthetic seed, different concentrations and combinations of phytohormones with MS media were used in seed bead to achieve optimum germination rate on MS0 media. Among the different concentrations and combinations, MS+1.0 mg L-1 BAP+0.1 mg L-1 GA3 gave the highest germination rate and it was 81% for Loda and 70% for China variety. The influence of storage at 4°C and 0°C temperature on germination rate was also examined. This investigation indicates that synthetic seed could be stored at 4°C temperature for 45 days.
  A.K.M.N. Huda , M. Rahman and M.A. Bari
  This study describes the investigation on the synthetic seed technology in two varieties (Loda and China) of eggplant with a particular focus on the carbon source in seed bead. To assess exact type and concentration of carbon source, three types of carbon sources (viz., sucrose, mannitol and sorbitol) and their different concentrations were used in alginate seed bead to achieve the optimum germination of synthetic seed on MS0. Among the different carbon sources, sucrose showed better performance for both varieties when nodal segment with bud was used as explants. But when somatic embryos were used as explants sucrose + sorbitol (1:1) was found to be more efficient for both varieties in germination of synthetic seeds. On the other hand, among the different concentrations 1% sucrose gave good result for the germination of synthetic seed made of nodal segment. In case of somatic embryo 3% sucrose showed best performance for both varieties.
  A.K.M.N. Huda , M.A. Bari , M. Rahman and N. Nahar
  This research programme was undertaken with a view to develop an efficient and reliable method of indirect regeneration through somatic embryogenesis in two varieties of eggplant through the technique of tissue culture. For this purpose, cotyledon pairs of Solanum melongena were used as explants for callus induction. Three different auxins (NAA, 2, 4-D and IAA) were used singly or in combination with BAP for present investigation, as types, concentrations and combinations of growth regulators had marked influence on callus induction. Auxin/cytokinin combinations were proved more potent for callus induction as well as callus growth than that of auxin tried singly in the media. Best callus formation was observed in MS media when supplemented with 0.05 mg L-1 BAP + 2.0 mg L-1 NAA. Best calli from cotyledonary explant were subcultured on MS supplemented with different combinations and concentrations of phytohormones for somatic embryogenesis. Among the different combinations and concentrations 0.05 mg L-1 BAP + 2.0 mg L-1 NAA gave the best result on somatic embryogenesis for Loda variety. On the other hand, somatic embryos of China variety were obtained only on the media having 1.0 mg L-1 BAP + 0.05 mg L-1 GA3. The global structure somatic embryos originated from the superficial layers of the calli, as a single structure or in a cluster. These structures could be isolated easily from parent tissues because they were floated freely in water. These floated free embryos were cultured on nutrient media again to obserb shoot primordia development and thereafter shoot development.
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