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Articles by A.K. Srivastava
Total Records ( 6 ) for A.K. Srivastava
  G. Lokesh , Putkho Paul Pao , K.N. Madhusudhan , P.K. Kar , A.K. Srivastava , M.K. Sinha , R. Manohar Reddy , P.M. Muniswamy Reddy and B.C. Prasad
  The wild silkworm, tropical tasar Antheraea mylitta Drury being mainly culturing in central and eastern parts of India and produces famous tasar silk. This insect species expresses divergent phenotypic characters in response to varying ecological and climatic conditions thus exists as ecoraces. Hence, in the present study, three ecoraces viz., Daba, Sukinda (Semi-domesticated) and Sarihan (wild) have been selected for the assessment of the variability in silk gland related traits. The parameters of the silk glands studied like comparative length, weight and silk gland to larval body mass index. Besides, some biochemical contents of the silk gland tissue were also studied in three ecoraces. The results showed significant variations at p<5% in all the parameters among the three ecoraces studied, also showed the sexual dimorphism in expression of silk gland features and economic traits. The semi-domesticated Daba ecorace showed higher performance in most of the parameters with comparatively greater in silk gland weight (22%) and in body mass index (>16%). Apart from this, wild ecorace Sarihan cocoons showed higher shell ratio (>10% over the value of other ecoraces). The study infers that, there is wide variability in the expression of phenotype with respect to silk gland related traits among the different ecoraces of tropical tasar silkworm A. mylitta. Since the silk glands are site of biosynthesis of silk proteins, these may be considered for the assessment of diversity or characterization of different ecoraces of tropical tasar silkworm.
  A.K. Bhardwaj , R.C. Bansal , R.K. Saket and A.K. Srivastava
  Power load forecasting is having its own importance in a bulk interconnected combined generation, transmission and distribution power system. Accurately load forecasting is important to establish operational plans for power stations and their generation units, implementation of improved plant scheduling. It further provides a reliable and credible resource that ensures stable and economical operation of the power. The crucial issue of managing the demand is of a great importance in formulating the future development policy for the whole country. The case studies analyses the requirement of electricity with respect to the future population for the major forms of energy in the KAVAL cities of Uttar Pradesh state in India. A model consisting of significant key energy indicators have been used for the estimation. Model wherever required refined in the second stage to remove the effect of auto-correlation. The accuracy of the model has been checked using standard statistical techniques and validated against the past data by testing for expost forecast accuracy. The study identifies the urgent need for special attention in evolving effective energy policies to alleviate an energy famine in the near future.
  R.M. Reddy , M.K. Sinha , K.P.K. Kumar , N.S. Gahlot , A.K. Srivastava , P.K. Kar and B.C. Prasad
  The aim of the present study is to explore hybridization influence to achieve the potential of silk yield and quality in commercial tasarculture, as against the current status of applying only a few wild and semi-domesticated ecoraces of Antheraea mylitta D to produce raw silk during commercial crop rearing season (September-December). The four F1 hybrid combinations generated using Daba, Jata and Raily tasar ecoraces of seed crop rearing season (July-August) were evaluated during commercial crop season of 2007 and 2008 for the influence of hybridization as positive heterosis in the traits of economically important silk productivity and filament denier. The F1 hybrid, Daba x Jata has recorded positive heterosis in single shell weight (+25.6%), total silk yield (+79.0%) and silk filament length (+68.1%), but for silk filament of high denier (11.98 d) followed by its reciprocal hybrid, JataxDaba in shell weight (+18.8%), silk yield (+68.1%), filament length (+63.8%) and filament of highest denier (12.32 d). Though, the RailyxDaba hybrid could show highest heterosis in shell weight (2.47 g) with filament of least denier (10.86 d), the improvement in filament length was marginal (+44.7%) and heterosis in silk yield was negative (-47.0%), while the performance of DabaxRaily hybrid was least among the hybrids. The study infers hybridization influence on silk related traits at F1 level as positive heterosis and the application feasibility of DabaxJata and JataxDaba hybrids during commercial crop rearing season for higher silk productivity with silk filament denier at least better than one of the parents of F1 hybrid combination.
  A. Kumar , S.V. Singh , A.K. Srivastava , N.K. Gangwar , P.K. Singh , S. Gupta , K.K. Chaubey , R. Tiwari , S. Chakraborty and K. Dhama
  Johne’s Disease (JD), caused by Mycobacterium avium subspecies paratuberculosis, is endemic in domestic animals and adversely affects dairy industry worldwide. In the present study, efficacies of ‘Indigenous’and commercial (Gudair, Spain) vaccines were evaluated for control of JD in experimentally challenged goats. Goats were grouped into Sham-immunized, Indigenous and Gudair vaccine groups. Vaccinated kids were challenged at 50 and 270 Days Post Vaccination (DPV), with 3×109 and 5×109 ‘Indian Bison Type strain ‘S 5’, respectively and sacrificed at 150 and 450 DPV after 1st and 2nd challenge, respectively. Vaccines were evaluated for improvements in physical condition (diarrhea, weakness, body coat color), clinical symptoms (shedding of bacilli, mortality, morbidity), immune responses (cell-mediated and humoral), pathology (gross and microscopic lesions) and production status (body weights, growth rates). Vaccinated goats gained higher body weights vis a vis sham-immunized. Mortality was higher in sham-immunized. Cell Mediated Immunity (CMI) response increased at 30 DPV and showed down regulation from 90 DPV onwards in vaccinated goats. Significant increase in humoral immune response was observed in vaccinated goats at 180 DPV and maintained till 450 DPV. Microscopical examination at 180 DPV showed reduced shedding in vaccinated groups, At 200 DPV, group 1 goats showed thickening of small intestine with corrugations specifically at ileocaecal junction, catarrhal enteritis with infiltration of mononuclear cells and epitheloid cells. In vaccinated groups, there were focal thickening of intestines at 450 DPV with lesions of chronic catarrhal enteritis and presence of lymphocyte, plasma cells and macrophages cells with a few epitheloid cells. Monitoring of MAP DNA in the blood of experimental goats of all the groups was done by testing of blood samples by Polymerase Chain Reaction (PCR) and the vaccinated groups of goats revealed MAP bacilli free status upto 300 DPV. Both the vaccines provided protection after challenge I, but since indigenous vaccine also protected goats after challenge II, was therefore superior. In conclusion, the indigenous vaccine must be exploited for its full potential for effective prevention and control of this economically important disease having public health concerns.
  R. Manohar Reddy , Suresh Rai , A.K. Srivastava , P.K. Kar , M.K. Sinha and B.C. Prasad
  Assorted F1 hybrids and reciprocals of divergent tasar ecoraces of Antheraea mylitta viz., Daba, Jata and Raily were evaluated for relative heterosis. The DabaxJata [R] (random female and male) and its reciprocal hybrids have recorded high positive heterosis for fecundity (+17.45 and +1.70%), egg fertility (+11.58 and +7.20%), shell weight (+34.76 and +27.44%), silk ratio (+30.49 and +24.15%) and silk yields (+94.33 and +82.51%), respectively. Dabax Jata [PxS] (high pupal female and high shell male) hybrid, although recorded positive heterosis for all traits, but was next to random parental hybrids. The DabaxJata [PxP] (high pupal female and high pupal male) combination and its reciprocal have shown positive heterosis only for fecundity (+22.13 and +35.89%), while DabaxJata [SxS] (high shell female and high shell male) hybrid and its reciprocal are positive in shell weight (+45.12 and +33.54%) and silk ratios (+26.95 and 27.95%). All hybrids of DabaxRaily including reciprocals have shown negative heterosis for fecundity and silk yields. However, DabaxRaily [R], [SxS], [PxS] hybrids and their reciprocals have shown uneven positive heterosis for egg fertility (+1.65 to +20.74%), shell weight (+7.56 to +56.98%) and silk ratios (+1.02 to +54%). In general, all reciprocal F1 hybrids of Daba, Jata and Raily ecoraces have shown lesser heterosis. The dissimilar performance of assorted F1 hybrids and reciprocals of Daba, Jata and Raily ecoraces reveal their varied potential on relative heterosis. However, the study infers commercial prospective and optimal seed cocoon expediency in Daba and Jata ecoraces as F1 hybrids [R] and reciprocals. While, the trait specific positive heterosis in high pupal and high shell hybrids (assorted F1 hybrids) have application in segregating lines with desired traits and aggregating them in to needy one(s).
  A.K. Yadav , S. Vardhan , M.S. Yandigeri , A.K. Srivastava and D.K. Arora
  Feathers are major byproduct waste of poultry industries and produced in large amount. Keratin can be biodegraded by keratinolytic microorganisms and in this study, 45 keratinase producing actinomycetes and their enzyme production was isolated from hot springs, Rajgir, India. After Screening of keratinase enzyme production, the strain showed highest activity was selected and identified as Streptomyces sclerotialus by using polyphasic taxonomy including morphological and biochemical characteristics followed by 16S rDNA and DNA directed RNA polymerase beta subunit (rpo β) gene sequencing (accession no: HQ827802 and HQ827815). The effect of various substrates, temperature, pH and protease inhibitors, reducing agents and metals ions supplements in the production medium on enzyme production was studied and found that starch (3.5%) at 120 h, gives maximum activity followed by, protease peptone (1.5%) at 144 h recorded. The optimum temperature and pH recorded 55°C and 9.0, respectively. The enzyme was stable at 55°C for 1 h. Metals like MgSO4, CaCl2 were found strong inhibitory effect; whereas, Na2SO3 found to be stimulatory for enzyme production. Gel electrophoresis analysis revealed that the crude enzyme was monomeric with only a single protein band of 46 KDa were observed after ammonium persulfate precipitation.
 
 
 
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