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Articles by A.H. Sharr
Total Records ( 1 ) for A.H. Sharr
  B.P. Keong , S.S. Siraj , A.H. Sharr and M.S. Fauziah
  Population study focusing the genetic diversity had been widely exploited for wild species but unfortunately little attempt was being made to understand the dynamics of genetic diversity on cultured aquarium species. This study was carried out to understand the dynamics of genetic diversity of cultured live-bearer fish in Malaysia. Taxonomic groupings of both Xiphophorus and Poecilia spp was validated using molecular markers. One hundred and thirty one samples of locally cultured live-bearer fishes which comprised of Xiphophorus maculatus, Xiphophorus helleri, Poecilia reticulata and Poecilia latipinna were sampled. The live-bearer fish samples were genotyped with 24 microsatellite (SSRs) primer pairs. Fifteen out of 24 SSRs primer pairs were able to amplify among the live-bearer fishes and were polymorphic. The average number of alleles was low (1 to 8). Differences between mean observed (HO) and expected (He) heterozygosity for the four species live-bearer fishes were small ranging from 0.02 to 0.06 suggesting no drastic reduction of heterozygosity in cultured fish. The pair-wise comparisons of FST between these four species of live-bearer in general was high (FST>0.25). Such observation was expected as the four tested species of live-bearer belong to different taxonomic groups. A clear distinction between the species of Xiphophorus and Poecilia as revealed by SAHN-clustering tree plot seems to be in accordance with their taxonomy. The data generated from this study provide useful information in understanding the levels of genetic variation in cultured aquarium fish for resource management and conservation.
 
 
 
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