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Articles by A.G. Ebadi
Total Records ( 16 ) for A.G. Ebadi
  M. Shokrzadeh and A.G. Ebadi
  In this study, we have evaluated the effects of cisplatin on the cellular total GSH level in different tumor and normal cell lines. Five different cell lines of human hepatic carcinoma (HePG2), human lung adenocarcinoma (A549), human ovarian carcinoma (SKOV3), dog kidney (LLCPK1), Chinese hamster ovary (CHO) and Human gingival fibroblast (GHF1) cell lines were exposed to their respected IC50 concentrations of cisplatin for two hours. cisplatin cytotoxicity was measured using clonogenic assay and the total cellular GSH level was analyzed using a photometrical assay. The results showed that cisplatin had different degrees of cytotoxicities on different cell lines as shown by IC50 values; o.87 for HepG2, 3.27 for A549, 0.99 for SKOV3, 5.50 for LLCPK1, 5.50 for CHO and 1.60 for GHF1 cell lines and GSH level alterations after exposure to cisplatin were also different for different cell lines that 85.33 for HepG2, 637.00 for A549, 2691.00 for SKOV3, 1388.30 for LLCPK1, 412.60 for CHO and 783.24 for GHF1 cell lines. This study showed that the cellular GSH level increased in LLCPK1, A549, SKOV3 and GHF1 cell lines, but decreased in CHO and HepG2 cell lines versus to the matched controls. The highest significant variation of GSH in cancer cell line was belonging to SKOV3 and in normal cell lines for LLCPK1, after treated with cisplatin. It is concluded that the total GSH variation after exposure to cisplatin is different for different cell lines. We were not able to correlate between the level of resistance to cisplatin (based on the IC50 levels) and GSH level or variations in this study. It might indicate the GSH neither the unique, nor the most important mechanism of resistance to cisplatin in these cell lines, in spite of many publications in its favor.
  M.R. Kalantari and A.G. Ebadi
  The Neka River watershed covers a large portion of Mazandaran province, Iran and is centrally important to both recreational and other activities in the region. In order to assess current water quality conditions distribution of three heavy metals were determined in river sediments at four sampling stations in the watershed. Cu, Cr and Fe concentrations were determined using flame atomic absorption spectrometry. Standard operating procedures for the analysis of metals were used. Metal concentrations in sediments ranged from 6.35 to 13.56 for Cu, 17.66 to 209.7 Cr and 1.5 to 23.4 for Fe mg g-1 dry weight sediment. The results showed higher concentrations of Cu, Cr and Fe in the test samples compared to that in the control.Significant difference in level of Cu, Cr and Fe was seen between test and control groups (p<0.05 for Cu and Fe ; p<0.001 for Cr). This study can be an alarm to researchers for more study about pollution of sediments in Mazandaran and finding proper methods for reducing and removing.
  M.R. Kalantari and A.G. Ebadi
  The extent of contamination of two great rivers (Tajan and Neka) of Mazandaran province in Iran by selected organochlorine compounds has been assessed through the analysis of surficial sediments taken from four sites of each river by using GC (Hewlett Packard HP5880) equipped with an electron capture detector and split/splitless injector. Concentrations of HCHs at sites influenced by the agricultural regions in Mazandarn province are among the highest recorded on a global basis (up to 30 ng g-1 dry wt.). The ratio between the a-and β-isomers was relatively low indicating contamination through the use of lindane. Concentrations of DDTs (73-91 ng g-1 dry wt. in Tajan and 49-81 in Neka) and PCBs (8-26 ng g-1 dry wt. in Tajan and 9-15 in Neka river) were not especially high in comparison to levels reported from throughout the world.
  M.R. Kalantari , M. Shokrzadeh , A.G. Ebadi , C. Mohammadizadeh , M.I. Choudhary and Atta-ur-Rahman
  Heavy metals and metalloids are an increasing environmental problem worldwide. Some industrial activities and agricultural practices increase their level in the substrate and the possible introduction of these elements in the food chain is an increasing human health concern. The protection and restoration of soils and water contaminated with heavy metals generate a great need to develop efficient adsorbents for these pollutants. Agricultural fertilizers which contain small amounts of cadmium and lead is widely applied and used in Iran. However, both these heavy metals remain below toxic levels. In contrast, contamination of lowland rice fields by sewage sludge from textile plants and some mining has increased the heavy metal content of the soil and reduced rice yields in these areas (Mazandaran province). Currently remediation of polluted soil is being carried out, using plants such as Vetiveria zizanioides and Eichornia crassipes, plus applications of zeolite in some areas of Meandering province of Iran. This mini review firstly indicate general objectives about remediation and then deal with to some activies about agricultural soil remediation that contaminated with some heavy metals (specially, Pb and Cd in Mazandaran province of Iran. We conclude that above mentioned species, may be an effective species for phytoextraction and should be tested for this purpose in field conditions.
  M.R. Kalantari and A.G. Ebadi
  In this study, determination of Pb, Cd and Zn was performed in sediment extracts of two great rivers in Mazandaran province (Iran) according to the Atomic Absorption Spectrophotometry procedure in eight sites.from each river four sites selected (2 for test and 2 controls). The results showed higher concentrations of Zn, Pb and Cd in the test samples compared to that in the control in two rivers. Significant different in level of Pb, Cd and Zn was not seen between two rivers (p<0.05). At sample site Tajan, which was the first test sample taken. This study can be a alarm to researchers for more study about pollution of sediments in Mazandaran and finding proper methods for reducing and removing.
  A.G. Ebadi and S. Zareand M. Babaee
  This study investigated the chloride oregano insecticide sprays such as heptachlor in the four of the most consumed kinds of fishes in the Caspian Sea. This investigation prepared four different kinds of fishes, which arc highly used and available in the Caspian Sea (Sefid, Koli, Kilca and Kafal fish) that we selected 100 samples of fish from the four different hunting region (Chalous and Babolsar city and Khazar Abad and Miankaleh regions) in 2004. After cleaning, we prepared Hun muscle of the samples, then after the process of extraction, we distilled them in vacuum by means of organic solvent and at last we evaluated the remaining and reported based on ppm by Gas Chromatography (GC) with ECD detector. The results of insecticides analyzed in four kinds of fishes and showed that Kafal in the hunting region of Khazar abad had greatest amount of 0.037 ppm. Kilca from Miankaleh had the highest amount of heptachlor, 0.026 ppm (p<0.05) in the case of Sefid and Koli fishes in regions, significant difference was not seen. It is necessary to mention that no research has sofar been done to be a criterion for comparison in this area, But, fortunately the amounts of the insecticide sprays has not reached to standard level in fishes and it raises the alarm health.
  S. Zare and A.G. Ebadi
  In this study samples of three commonly consumed fish ( Sefid, Kafal and Koli ) were analysed for concentrations of heavy metals (Pb, Cd and Cu). Fish were captured using electric fishing on four sites along the Tajan River (Mazandaran province). The concentrations of heavy metals were determined by atomic absorption spectrophotometer. Samples contained detectable concentrations of heavy metals but at concentrations below the Maximum Residue Limit (MRL). Cadmium concentrations were below the MRL. Lead contamination showed no significantly different. Copper contamination was correlated with the localization of industrial plants. With respect to concentrations of pollutants in the Tajan river should not pose a serious threat to the fishes and public health.
  A.G. Ebadi and S. Zare
  In this study concentrations of Parathion (organophosphorous pesticides) was determined in four commercially valuable fish species, Rutilus frisikutum, Clupeonella delicatula, Mugila auratus and Vimba vimba, from four sites of Tajan River in July 2004. The concentration of parathion was measured by gas chromatography electron-capture detection (GC-ECD). Concentrations of the parathion in examined fish species ranged from 5.94 (site III) to 49.57 (site IV) lg kg-1 (wet weight). No difference found between kind of fishes in each sites about parathion concentrations but there are two groups of sites that were significantly different from one another in terms of parathion concentrations: sites 2, 3 and 4 < site 1 (p<0.05). Parathion in the edible parts of the investigated fish were in the permissible safety levels for human uses and should not be public concern among peoples in Mazandaran province.
  A.G. Ebadi and M. Shokrzadeh
  The effect of chronic exposure to menazon (Organophosphorus compound) in the production of oxidative stress was assessed in rats. Administration of menazon (50, 100, 500, 1000 ppm) for 2 weeks duration increased catalase (CAT), superoxide dismutase (SOD) activities in red blood cells (RBC). However, acetylcholinesterase (AChE) activity were decreased in these samples. The increase in RBC lipid peroxidation correlated well with the inhibition in RBC AChE activity. Increased activities of CAT and SOD showed significant correlations in RBC samples when different doses of menazon were used. The results of the present study suggest the usefulness of RBC AChE measurement as a good index in the evaluation of menazon-induced oxidative stress affecting blood.
  M. Shokrzadeh and A.G. Ebadi
  Garlic (Allium sativum L.) has an important dietary and medicinal role for centuries. It is a large annual plant of the Liliaceae family, which grows in most of Europe and in northern Iran. Iranian garlic is used in traditional medicine for infectious diseases, flu and as an anti-febrile. The present study tested the aqueous extract of garlic in vitro for its antibacterial activity. The extract showed concentration-dependent antibacterial activity against Staphylococcus aureus 8327. This activity was heat resistant, but the activity of freeze-dried extract gradually diminished during a 90 days period. The traditional use of Iranian garlic for infectious diseases and for controlling fever appears to be justified.
  M. Shokrzadeh , A.G. Ebadi , S.S. Mirshafiee and M.I. Choudhary
  Camellia sinensis or tea belonging to the family of Theaceae, is widely grown. In Mazandaran provinces in the north of Iran. Tea is known in folk medicine as a medicinal plant that used as hypotensive and anti-diabetic. In this research the aqueous green leaf extract of Camellia sinensis (450 mg kg-1) showed a strong glucose lowering effect after oral administration in rats. The decrease of glycemia has reached to 30% of the control value 2 h after glucose loading. The amount of glucose absorbed in a segment jejunum in situ was 9.2±0.2 mg in presence of tea extract vs. 14.11±0.91 mg in control rats during 2 h (p<0.05). The results indicate that aqueous extract of tea has a significant anti hyperglycemic effect that may be caused in part by the reduction of intestinal glucose absorption.
  M. Skokrzadeh , F.H. Shirazi , M. Abdollahi , A.G. Ebadi and H. Asgarirad
  One of the well-known cellular defenses after exposure to cytotoxic agents is the glutathione (GSH) related mechanisms. Resistant to cisplatin (DP) chemotherapy has been strongly correlated to GSH-mediated mechanisms in many articles. In this study, we have evaluated the effects of cisplatin on the cellular total GSH level in different tumor and normal cell lines. Five different cell lines of human hepatic carcinoma (HePG2), human lung adenocarcinoma (A549), human ovarian carcinoma (SKOV3), dog kidney (LLCPK1), Chinese Hamster Ovary (CHO) and Human gingival fibroblast (GHF1) cell lines were exposed to their respected IC50 concentrations of cisplatin for two hours. Cisplatin cytotoxicity was measured using clonogenic assay and the total cellular GSH level was analyzed using a photometrical assay. The results showed that cisplatin had different degrees of cytotoxicities on different cell lines as shown by IC50 values; 0.87± 0.07 for HepG2, 3.27±0.35 for A549, 0.99±0.08 for SKOV3, 5.50±0.35 for LLCPK1, 5.50±0.21 for CHO and 1.60±0.21 for GHF1 cell lines. GSH level after exposure to cisplatin (GSH-DP) were also different for different cell lines compare to their controls (GSH-C); 85.33±8 for HepG2, 637.00±81 for A549, 2691.00±416 for SKOV3, 1388.30±261 for LLCPK1, 412.60±32 for CHO and 783.24±30 for GHF1 cell. It is shown that compare to the matched controls, the cellular GSH level increased in LLCPK1, A549, SKOV3 and GHF1 cell lines, but decreased in CHO and HepG2 cell lines. The highest significant variation of GSH in cancer cell line was belonging to SKOV3 and in normal cell lines to LLCPK1, after treated with cisplatin. It is concluded that the total GSH variation after exposure to cisplatin is different for different cell lines. We were not able to correlate between the level of resistance to cisplatin (based on the IC50 levels) and GSH level or variations in this study. It might indicate that in spite of many publications so far, the GSH is neither the unique, nor the most important mechanism of resistance to cisplatin in these cell lines. Internal and Eternal GSH level in Studied cell lines will be changed in several ways when contaminated with different concentration of vitamins (for examples, Vit C, Vit E and Vit C+E) and observed that variation was more prominent in cancer cell line.
  M. Shokrzadeh and A.G. Ebadi
  Antioxidants in rice food are important for human health. However, the level of antioxidative activity of different rice grains (Oriza sativa L.) which is the staple food in Mazandaran province of Iran and is the main agricultural product exported to other countries has not previously been reported. In this preliminary investigation, the antioxidative activity in vitro of the alcoholic extract from four different kinds of rice grains have been determined by ABTS/methemoglobin method compared with Trolox, an vitamin E analog. It was found that the antioxidative activity (TEAC) as μmol per g of dry rice varied from the highest to the lowest as the followings: Tarom rice (20.22), Khazar rice (9.44), Neda rice (8.78) and Sadri rice (1.33), respectively. TEAC is distinguishly the highest in Tarom rice and remarkedly high in Khazar rice. This property may be due to the high contents of rice anthocyanins, vitamin E, tocotrienols and oryzanol. The functional chemistry, nutritional value and health benefits of antioxidants contained in rice grains, rice bran and their products should be intensively studied and characterized for their ingredients and stability. Further development for the value addition of rice as diet supplements and maximum health benefits is needed.
  A.G. Ebadi , S. Zare , M. Mahdavi and M. Babaee
  One of the effects of industrialization of communities is great consumption of different chemical substances that resulted severe consequences such as, chronic poisoning that most of them will be dangerous and life threatening. The metals are part of major substances with mutational and carcinogenic effects. With respect to the collective characteristics of these metals on human and environmental health, we decided to evaluate their adverse effects. Present study was done in Gillan province (North of Iran) on green leaf of Tea cultivated in Lahijan and Fuman cities. The samples were selected from ten regions and five samples from selected areas were studied. evaluation of metals in green leaves (Pb, Cd, Cr and Zn) was carried out by atomic absorption spectrometry .The results showed a significant relation between the amount of lead and regions (P < 0.05) and also comparison between these mean numbers showed that in Eastern station, lead had the highest amount with average of 5.300 ppm in Fuman and 4.300 ppm in Lahijan samples. Amounts of other metals (Cd, Cr and Cu) were below standard that proposed by WHO. With respect to obtained results, there is a great potential for hazardous substance in the studied regions and it is necessary to make a serious analysis about this substances in other regions of Gillan Province.
  A.G. Ebadi , M. Shokrzadeh and S. Zare
  In this study samples of Cucumber (Cucumis sativus L.) were analyzed for concentrations of lindane (organochlorine pesticides). Samples were gathered on four sites in Sari city (north, south, east, west and central areas) in Mazandaran province of Iran. Quantitative determination of the lindane was performed by gas chromatography electron-capture detection (GC–ECD). Samples contained detectable concentrations of lindane but at concentrations below the proposed limit by WHO. No significant difference was found between sites about lindane concentrations but Central area showed maximum level in lindane concentrations (p < 0.05). Concentrations of lindane in cucumber sampled in this study should not be public concern among peoples in Mazandaran province.
  M. Shokrzadeh and A.G. Ebadi
  In this study samples of four species of the most consumed fishes (Sefid, koli, kilca and kafal fish) were analyzed for concentrations of Chlorobenzilate (organochlorine pesticides). Fish were captured using electric fishing on four sites (Chalous and Babolsar city and Khazar Abad and Miankaleh regions) in Mazandaran provinces of Iran. Quantitative determination of the Chlorobenzilate was performed by gas chromatography electron-capture detection (GC–ECD). Samples contained detectable concentrations of Chlorobenzilate but at concentrations below the maximum residue limit (MRL). No different found between kinds of fishes in each sites about Chlorobenzilate concentrations but there are two groups of sites that were significantly different from one another in terms of Chlorobenzilate concentrations. According to insecticides analyzed in four kinds of fishes the Kafal in the hunting region of Khazar abad had greatest amount of 0.038 PPM. Kilca from Babolsar had the highest amount of Chlorobenzilate, 0.035 PPM (P< 0.05). In the case of Sefid and Koli fishes in regions, significant difference was not seen. It is necessary to mention that no research has so far been done to be a criterion for comparison in this area sites. However, the concentrations of Chlorobenzilate residues in the muscle were found to be lower than the FAO/WHO recommended permissible and should not be public concern among peoples in Mazandaran province.
 
 
 
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