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Articles by A.E. Abdel-Khalek
Total Records ( 7 ) for A.E. Abdel-Khalek
  Z.M. Kalaba and A.E. Abdel-Khalek
  The information in the literature on the effect of light colour on productive and reproductive performances of rabbit production is scare. It was suggested that evaluation of the light colour’s effect on the rabbit production can be perspective. This study aimed to evaluate the effect of florescent (FL) and red (RL) compared with Natural Light (NL) on reproductive performance of rabbit does and growth performance of their kits. Total of 75 mature California does were divided into three groups (25 in 5 replicates for each) in separated rooms. Control does were exposed to natural day light (G1), while those in G2 and G3 were exposed to artificial fluorescent or red lights, respectively for 16L/8D regime. Kindling Rate (KR), Gestation Period (GP) and Litter Size (LS) of does as well as weight (LW), Viability Rate (VR) and gain of kits was recorded at birth and weaning for three consecutive litters. Blood samples were collected pre-insemination for the 2nd parity from 10 does in each group for determination of melatonin, FSH and oestrogen concentration in plasma. Values of KL, reproductive index of does, LS, VR and LW of kits at birth and LS, VR, weight and gain of kits at weaning were the highest (p<0.05) and GP was the shortest (p<0.05) for RL does. Artificial lights (FL and RL) increased (p<0.05) melatonin, FSH and oestrogen concentrations. In conclusion, using industrial red light resulted in increasing reproductive performance of rabbit does and weight and viability of their kits.
  Z.M. Kalaba and A.E. Abdel-Khalek
  Various hormonal treatments were used for induction of ovulation to increase fertility of rabbits. Aim of the present study was to investigate the effect of repeated GnRH injections or Intra-Vaginal Mechanical Stimulation (IVMS) at mating for six consecutive litters, on fertility of naturally mated New Zealand White rabbit does. Does failed to kid after one service were mated in the next parity. Kindling Rate (KR), Number of Total (NTB) and Live Borns (NLB), Viability Rate (VR) and average Kit Weight (AKW) at birth as well as Gestation Period Length (GPL) were recorded after kidding for six consecutive parities. Also, concentrations of Follicle Stimulating Hormone (FSH) and Luteinzing Hormone (LH) were determined after mating of the 2nd litter. Results reveled that GnRH treatment showed highest (p<0.05) KR (71.4%), NTB (6.16/litter), NLB (5.53/litter) and VR (89.8%) and shortest (p<0.05) GPL (30.93 d). However, IVMS showed highest (p<0.05) AKW (66.59 g). Highest (p<0.05) VR was at the 1st and 2nd parity (87.5 and 84.5%), lowest at the 3rd parity (73.8%) and modest between 4th-6th parity. At 2, 3 and 4 h post-mating, FSH concentration was 12.04, 12.90 and 9.58 ng mL-1, while LH concentration was 16.0, 10.38 and 9.63 ng mL-1, respectively. The current study indicated that repeated injection with 0.2 mL GnRH analogue/doe (Receptal) at mating for 6 consecutive litters resulted in the best reproductive performance of rabbit does, leading to improving fertility of NZW does.
  W.A. Khalil , Sh. A. Gabr , Sh. M. Shamiah , A.M.A. El-Haif and A.E. Abdel-Khalek
  This study aimed to compare the efficiency of three cryodevices (Conventional Straws (CS), Hemi-Straws (HS) and spatula (SP)) in vitrification of buffalo oocytes. Oocytes were recovered from ovaries of slaughtered buffaloes. They were vitrified and thawed according to each cryodevice used. Then they were in vitro matured, fertilized and cultured to blastocyst stage. Survival and quality, in vitro maturation, fertilization rate and blastocyst production rates of vitrified immature buffalo oocytes were assessed. Results showed that total survival rate of the vitrified oocytes was higher (p<0.05) for SP and HS (96 and 95%) than CS (80%). Recovery rate of normal oocytes relative to vitrified or survival oocytes was the highest (p<0.05) for SP compared to other cryodevices. In vitro maturation rate (oocytes at metaphase II stage) was lower (p<0.05) for all vitrified oocytes than fresh (61.1 vs. 27.8-44%). SP showed higher (p<0.05) maturation rate (44%) than HS and CS (34.4 and 27.8%, respectively). Cleavage rate was lower (p<0.05) for all vitrified oocytes than fresh oocytes (37.7-58.9% vs. 70.8), being higher (p<0.05) for SP and HS (55.8 and 58.9%, respectively) than for CS (37.7%). Blastocyst production rate relative to cleaved oocytes was lower (p<0.05) for vitrified than fresh oocytes (5.9-14.3% vs. 31.3) but the differences among vitrification cryodevice were not significant. In conclusion, immature buffalo oocytes can vitrified successfully by spatula, conventional straws and Hemi-straws. Spatula device is a reliable alternative, inexpensive and easy to assemble for vitrification of immature buffalo oocytes. Also, vitrification spatula has the largest effective holding capacity.
  Sh. A. Gabr , A.E. Abdel-Khalek and I.T. El-Ratel
  This study aimed to evaluate the influence of collection techniques (dissection, aspiration, slicing and aspiration plus slicing) on yield and quality of buffalo oocytes in vitro matured (IVM) in TCM-199 or Ham’s F12 with or without hormones (follicle-stimulating hormone, FSH and estradiol, E2). Recovery Rate (RR) and category of the collected oocytes were determined and only Cumulus Oocyte Complexes (COCs) were matured in vitro. Results showed that RR was 84.6, 83.3, 72.7 and 52.0% for aspiration plus slicing, slicing, aspiration and dissection technique, respectively (p<0.05). Percentage of COCs was higher by slicing than aspiration, aspiration plus slicing and dissection (63.2 vs. 51.3, 51.2 and 42.0%, p<0.05). The corresponding percentages of expanded oocytes were 29.9, 30.3, 27.6 and 32.7%, respectively (p<0.05). Percentage of oocytes arrested at metaphase-II (MII) was higher (67.1%, p<0.001) while those at Germinal Vesicle (GV) (8.1%, p<0.001) and Deg. (9.1%, p<0.01) were lower in TCM-199 than in Ham’s F12. Hormonal addition increased percentage of oocytes arrested at metaphase-I (MI) and MII (p<0.05 and p<0.001, respectively) and decreased those at GV and Deg (p<0.001). Such results may indicate efficacy of slicing technique as a collection method on quantity and quality of buffalo oocytes. Also, in vitro maturation of buffalo oocytes was improved in TCM-199 supplemented with hormones (FSH and E2).
  A.E. Abdel-Khalek , I.T. El-Ratel , W.M. Wafa , H.A. El-Nagar , G.E. Younan and Sara F. Fouda
  To study ovulatory response and genital and in vitro embryo characteristics of control rabbit does (G1) compared with those treated with Coenzyme Q10 (10 mg CoQ10/kg LBW, G2) and L-Carnitine (40 mg LC kg–1 LBW, G3). Total of 15 mature NZW rabbit does (5.3 mo of age, 3.35 kg LBW) and 6 NZW bucks (7.0-9.0 mo of age and 3.85 kg LBW) were used in this study for 21 days prior to natural mating. All does were slaughtered 64-66 h post-mating to determine genital measurements and follicular and Corpora Lutea (CLs) number, Ovulation Rate (OR) and embryo Recovery Rate (RR) rates. By flushing the oviducts, embryos was counted and evaluated. Also, post-thawing Survival Rate (SR) and Viability Rate (VR) rates were determined. Results showed that dose in G2 showed greater number of follicles (18.2), CLs (13.6) and embryos (16.3) per dose and the highest OR (74.7%), RR (100%), excellent embryos (86.8%) and the lowest good and fair embryos (10.4% for each) compared with G1. Genital characteristics were not affected. Post-thawing SR was not affected, while VR was higher (p<0.05) in G2 and G3 than in G1 (88.9 and 87.5 vs. 76.9%). In vitro blastocyst production rate was higher (p<0.05) in G2 and G3 than in G1 (64.58 and 60.00 vs. 52.63%). In conclusion, pre-conception daily oral dose of rabbit does for 21 days with 10 mg CoQ10/kg LBW has impact on yield, quality, survival and viability of vitrified embryos and in vitro blastocyst production.
  I.T. El-Ratel , A.E. Abdel-Khalek , M.A. El-Harairy , Sara F. Fouda and Lamiaa Y. El-Bnawy
  Background and Objective: Green tea has very strong antioxidant, antibacterial, antiviral, enzyme inhibitory, antiradiation and anticancerous properties. This study aimed to evaluate the efficacy of different levels of green tea extract on reproductive performance, hematology, lipid metabolism and histological structure of liver and kidney of rabbit does. Methodology: Total of 30 mature does and 10 fertile bucks of New Zealand White (NZW) rabbits were divided into 3 groups, 10 in each. The 1st group was control, while second and third groups were given, 1 mL distilled water containing 200 and 400 mg kg–1 b.wt. of green tea extract, respectively, orally for 30 days prior to natural mating. Doe weight at start and end of treatment, mating, kindling and weaning and kid weight at birth and weaning were recorded. Doe litter size and kid viability rate at birth and weaning were calculated. Hematological parameters and lipid profile were determined in blood plasma of does after weaning. Histological structure of liver and kidney of does was examined. Results: Doe body weight was higher in control and second than in third group at end of treatment, mating and kindling (p<0.05) and at weaning (p>0.05). Kindling rate, gestation period and litter size of does and body weight and viability rate of kids did not differ. Hemoglobin concentration, hematocrit, mean corpuscular volume and white blood cells count increased (p<0.05), while platelet count decreased (p<0.05) in third than in control and second groups. Plasma total lipids, total cholesterol, triglycerides and high density lipoprotein concentrations decreased (p<0.05), while low density lipoprotein concentration increased (p<0.05) in second and third than in control group. No pathological legions were noted in the histological structure of liver and kidney. Conclusion: Daily oral administration of rabbit does 30 days prior to mating with green tea extract at levels of 200 or 400 mg kg–1 is recommended to improve reproductive performance and lipid metabolism of rabbit does without adversely effects on hematological parameters and liver and kidney function.
  A.E. Abdel-Khalek , H.K. Zaghlool and Sh. A. Gabr
  This study aimed to evaluate the effect of daily oral administration of free L-carnitine (LC) for 3 months on digestibility coefficients, blood parameters and semen traits of Friesian bulls. Total of 9 bulls (360±32.1 kg LBW and 20±1.4 months of age) were assigned into 3 groups. Bulls in G1, G2 and G3 were fed the same diet and kept under the same conditions but differed in LC level (0, 1 and 2 g h-1 day-1, respectively). Bulls were received LC for 3 months (treatment period). At the end of treatment, semen was collected from bulls twice a week for 12 weeks and evaluated for ejaculate volume, sperm progressive motility, livability and abnormality, sperm concentration and total sperm output. Also, digestibility coefficients were performed and blood samples were collected. Concentration of Total Proteins (TP), albumin (AL), globulin (GL), urea-N, cholesterol (CHO), glucose and Total Lipids (TL), activity of AST and ALT and testosterone were determined in blood serum. Results show that bulls in G3 showed highest (p<0.05) digestibility coefficients. Only concentration of TP and GL increased (p<0.05), AL/GL ratio and CHO and TL concentrations reduced (p<0.05) in LC groups. All semen characteristics improved (p<0.05) in LC groups, being better (p<0.05) in G3 than in G2. Serum testosterone concentration was higher (p<0.05) in G2 and G3 than in G1. In conclusion, oral dose of LC at a level of 2 g h-1 day-1 for 3 months had impact to achieve high quality semen to spread the use of artificial insemination with bulls of high fertility.
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