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Articles by A.E. Karrar
Total Records ( 4 ) for A.E. Karrar
  I.E. Aradaib , A.E. Karrar , M.A. Al-Dubaib , H.A. Musa , S.K. Kafi , A. El-Kadarou , A. Ashmaig and H.H. Abu-Aisha
  A Reverse Transcriptase (RT) Polymerase Chain Reaction (RT-PCR)-based assay was developed and evaluated for detection of Rift Valley Fever Virus (RVFV) ribonucleic acid (RNA). A pairs of oligoribonucleotide primers (RVFV1 and RVFV2), selected from the Small (S) RNA genome segment of RVFV virus, were designed and used as a target for PCR amplification. The primers RVFV1 and RVFV2 resulted in amplification of a primary 490 base pair (bp) PCR product. The PCR products were amplified from RNAs extracted from RVFV field isolates and vaccine strains, propagated in Vero cell cultures. Amplification products were not detected when the RT-PCR-based assay was applied to RNA from other related hemorrhagic fevers viruses including, Crimean Congo Hemorrhagic Fever (CCHF); dengue virus; epizootic hemorrhagic disease virus and total nucleic acid extracts from uninfected Vero cells. The described RT-PCR-based assay provides a rapid, sensitive and specific assay for detection of RVFV in cell culture and should be recommended for inclusion during an outbreak of the disease among humans and susceptible livestock.
  A.Ballal , A.E. Karrar and A.M. ElHussein
  Serum samples were collected from commercial layer and broiler flocks 3 to 63 weeks old at four areas from Sudan. All flocks were not previously vaccinated against infectious bronchitis (IB) virus. Sera were tested using haemagglutination inhibition (HI) test for IB virus antibodies. 689(71%) out of the 957 samples examined had an HI titer ranging from 6 to 11 (log ). High incidence (90%) of IB virus infection was detected among layer flocks at Khartoum State and 46.8% of the samples had an HI titer > 10(log ). Low HI titer (<4 log ) was detected among samples collected from layer flocks at Sennar (Central Sudan). The results obtained indicated widespread and distribution of IB virus infection in Sudan.
  A. Ballal , A.E. Karrar and A.M. ElHussein
  Outbreaks of suspected IB disease occurring in a number of commercial layer flocks at Khartoum and El Gazira states were studied during years (2000- 2001). Affected birds were of different breeds (Lohmann, Bovans Hisex). Their ages were from 5 / –7 months. The main clinical signs varied from mild respiratory signs to diarrhea sometimes yellowish in colour. Drop in egg production (up to 60%) was recorded in some flocks. IB virus was isolated from these outbreaks. Using virus neutralization (VN) and haemagglutination inhibition (HI) tests, three of these IB field isolates (M114/2000, K170/2000 and K158/2001) were antigenically similar to IB virus strain 4/91 (Neutralization index 4.4 to 4.8 and VN titer > 11(log ) were recorded). Isolate (K110/2000) on the other hand, was antigenically similar to IB virus Mass. (M41) strain (HI titer >11 log was recorded). This 2 is the first time to identify IB virus strain antigenically similar to IB virus strain 4/91 in Sudan.
  Amira M. Elhassan , M.A. Fadol and A.E. Karrar
  During this study swab samples were tested for demonstration of Infectious Bovine Rhinotracheitis/ Infectious Pustular Vulvovaginitis (IBR/IPV) virus (BHV-1) from different regions of the Sudan. Samples were collected from cases suffering from infertility, nasal discharges, vaginal discharges, lacrimation and abortion. The virus was isolated from five cases with typical signs of the disease including two from Niyala, two from Atbara and one from West Kordofan. Also using SNT, ELISA and PHA testes neutralizing antibodies were found, which confirmed the presence of the disease.
 
 
 
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