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Articles by A.D. Khosravi
Total Records ( 9 ) for A.D. Khosravi
  M. Mehdinejad , A.D. Khosravi and A. Morvaridi
  The aim of present study was to investigate the type of bacteria isolated from blood cultures and determination of their antibiotic susceptibility pattern. During 18 months, 2790 blood culture samples were screened. The positive blood cultures were examined and the organisms were identified as per standard procedures. Antimicrobial susceptibility testing was performed for all isolates by use of disk diffusion technique, according to CLSI guidelines. From total blood culture samples, 155 (5.6%) were positive. The most common isolated gram negative bacilli were Klebsiella pneumoniae 52 (33.5%), Eschericia coli 32 (20.6%) and Enterobacter sp. 15 (9.7%) and coagulase negative staphylococci (CONS) as predominant gram positive cocci, all the isolated bacteria showed the highest degree of resistance to ampicillin (98.7%), cefalexin (70.3%) and trimethoprim- sulfamethoxazole (69.7%). Gram positive cocci were also fully resistant to penicillin. In conclusion, present study revealed that both gram positive and gram negative bacteria were responsible for bloodstream infections and most of the strains were multi-drug resistant. The most common isolated bacteria from blood cultures were Klebsiella pneumoniae and E. coli. Ciproflexacine was the most effective antibiotic against gram negative bacilli, while vancomysin was mostly effective against gram positive cocci.
  N. Jomezadeh , A. Farajzadeh Sheikh , A.D. Khosravi and M. Amin
  The aim of present study is to apply the PCR method for detection of stx1 and stx2 genes in E. coli strains isolated from stool samples. Shiga Toxin-producing Escherichia coli (STEC) have emerged as pathogens that can cause food-borne infections and severe and potentially fatal illnesses in humans, such as Haemorrhagic Colitis (HC) and Haemolytic Uraemic Syndrome (HUS). Of the numerous serotypes of E. coli that have been shown to produce stx, E. coli O157:H7 are frequently implicated in human disease. So, due to serious infections associated with STEC in human, early recognition of STEC is critical for effective treatment of patients. In total 130 isolates of E. coli were recovered from patients by using standard identification procedures. The isolates were subjected to PCR technique for investigation of presence stx1 and stx2 genes.Results revealed the presence of stx1 in 20 isolates (35.5%), stx2 in 28 isolates (49.1%) and stx1 and stx2 both in 9 isolates (15.7%). The present study shows the relatively high prevalence of STEC producing stx1 and stx2 in the region of study. The higher detection rate of stx2 represented the important role of this gene in diarrheal diseases.
  M. Amin , M. Jorfi , A.D. Khosravi , A.R. Samarbafzadeh and A. Farajzadeh Sheikh
  Lactobacillus is a genus of lactic acid bacteria described as a heterogeneous group of regular non spore forming gram positive rods and found in a great variety of habitats such as plants and gastrointestinal tracts. The aim of this study was to isolate lactobacilli from plants and determine their inhibitory effect against some pathogens. Sixty lactobacilli isolates from fresh vegetables were enriched in Man-Rogosa-Sharpe medium (MRS) broth and isolated by growing on MRS agar medium and characterized by phenotypic characteristics and PCR technique at genus and species levels. The antimicrobial substance was extracted with ethyl acetate solvent and the antimicrobial activity against some pathogenic bacteria such as Escherichia coli, Salmonella typhi, Shigella dysenteriae, Bacillus anthracis and Staphylococcus aureus were investigated. Forteen L. plantarum and eight L. casei which were isolated from fresh vegetables were identified by PCR. Antimicrobial substance from MRS broth medium was extracted. This antimicrobial compound showed a potent inhibitory activity against all tested bacteria. The inhibitory substance was distinct from bacteriocins, lactic and acetic acids which are produced by these bacteria. In conclusion, fresh vegetables may be used as a source of antimicrobial lactic acid bacteria. L. casei and L. plantarum as two probiotics can establish themselves in gut and urogenital tract and prevent the human body from adverse effects of pathogens.
  S.J. Emam , A.D. Khosravi and A. Alemohammad
  The aim of this study was to evaluate the effect of venom H. lepturus on hematological changes and renal failure. Results of this study will help us in quick diagnosis and treatment of scorpion stinging and decreasing the mortality. In total 119 patients were studied. Various blood parameters such as hemoglobin (Hb), Red Blood Cell (RBC) count, Prothrombin Time (PT) and Partial Prothrombin Time (PTT) and also urine parameters were measured. The highest number of victims was in age group of 20-29 years old. Hb was less than 12 g dL-1 in 54% of patients. The RBC count was 4.6 millions mm-3 in 48.73% of victims. PT in 61.4% of patients was more than 13 and it showed that there is a significant difference with the other group of stung victims (p = 0.001). PTT test in 43.7% of patients was normal. Severe hemoglobinuria was shown in 61.6% of H. lepturus stung victims, but 38.4% had a mild hemoglobinuria. 43.1 and 57% of patients had mild and severe hematuria, respectively. The results have been explained on the basis of damages to structures such as erythrocytes, coagulation factors and capillary system of glomeruli.
  A.D. Khosravi , J. Rostamian and P. Moradinegad
  The aim of present in vitro study was to investigate the antibacterial effect of low level laser of Galium Aluminium Arsenide (GaAlAs) on cariogenic bacteria. Suspensions of Streptococci mutans, Streptococci sanguis, Streptococci subrinus, Streptococci salivarius and Lactobacillus sp. were exposed to a GaAlAs (890 nm) in the presence of photosensitizer toluidine blue O in different time points of 2, 5, 12, 16 and 20 min. Viable microorganisms were counted on muller hinton agar plates after overnight incubation at 37°C and reported as colony forming unit. For all tested bacterial strains, at time points of 5, 12, 16 and 20 min, there was a significant decrease in the viable counts, reaching to a maximum value of 100% after 20 min, when treated with combination of laser + TBO (toluidine blue O). The combination of laser and TBO had the least effect on Lactobacillus sp. in time points of 5, 12 and 16 min, whereas it was more effective against S. mutans and S. sanguis at the same time points. Based on the presented results, we may conclude that the low level laser of GaAlAs was effective in significantly reducing of the viability of oral cariogenic bacteria in vitro.
  S. Nikakhlagh , A.D. Khosravi , A. Fazlipour , M. Safarzadeh and N. Rashidi
  The aim of this study was to determine the microbiology of CSOM in patients diagnosed with chronic otitis media referred to Otorhinolaryngology Clinic. Samples for culture were taken from 50 patients with CSOM. The samples were cultured on enriched differential and selective media and kept in both aerobic and anaerobic conditions. Conventional biochemical tests were then performed on isolated colonies. Drug susceptibility testing was done according to standard protocol. Forty one (82%) of the middle ear cultures were positive. The most common isolated aerobe was Staphylococcus aureus (32.4%) followed by Pseudomonas aeruginosa (21.6%). In the anaerobic group (12%), the peptococcus species was most prevalent. The most effective antibiotic in the aerobic isolates was ofloxacin. According to the results of drug susceptibility test, 95.5% of strains including all the S. aureus isolates were sensitive to ofloxacin. The present study was one of the extensive reports on both aerobic and anaerobic bacteria in CSOM. Knowledge of the pathogens, responsible for CSOM and choose suitable antibiotics according to susceptibility tests should guide the management of disease treatment and reduces intracranial and extra cranial complications with CSOM.
  M. Mehdinejad , A.D. Khosravi and A.Z. Mahmoudabadi
  The aim of this study was to determine the presence and nature of bacterial flora on hearing aids earmoulds in a children’s population. The study population consisted of 119 children referred to Imam Ali and Rudaky Schools in Ahvaz, Iran. Three samples were taken from surface of hearing aids earmoulds; canal in hearing aid wearers and ear without hearing aids earmoulds. The samples were cultured directly onto blood agar and MacConkey agar plates. According to preliminary examination, necessary standard biochemical tests were performed on grown bacteria and the organisms were identified as per standard identification criteria. Totally, 66 samples (61.1%) from hearing aids earmoulds and 124 samples (52.1%) from both ear canal without hearing aids earmoulds were culture positive, which 73(58.9%) and 51(41.1% ) were from right and left ears, respectively.The majority of isolated bacteria from earmoulds were Coagulase-Negative Staphylococci (CNS) 40(60.6%) and Polybacterial flora 14(21.2%) and the least isolated bacteria were Pseudomonas aeruginosa and Staphylococcus aureus. In conclusion, although the majority of isolated bacteria were common normal flora of the ear, however a few pathogens were isolated as well. So, it is very important to educate the people with hearing aids earmoulds about proper cleaning and disinfection procedure to prevent any serious ear canal infection.
  A.D. Khosravi , F. Ahmadi , S. Salmanzadeh , A. Dashtbozorg and E. Abasi Montazeri
  The aim of the present study was, to determine the bacteriology of orthopedic implant infections and susceptibilities of isolated bacteria to the commonly used antimicrobial agents. One hundred and sixty five patients were investigated for early or late postoperative infections of orthopedic bone implants using conventional microbiological procedures. Antimicrobial susceptibility testing were then performed for the isolated bacteria according to the standard guideline. A total of 155 isolates were recovered (152 aerobes and 3 anaerobes). Staphylococcus aureus, Klebsiella ozaenae and Pseudomonas aeruginosa were the most common causative agents. In relation to onset of infection, about 72.9% of patients were with early; 22.6% with delayed and 4.5% with late infections. The correlation between infection onset and total number of isolated bacteria was found to be statistically significant. The majority of isolated bacteria were sensitive to vancomycin, ciprofloxacine and imipenem. In conclusion, present study showed that S. aureus was the most common recovered bacterium with high sensitivity to vancomycin as expected. knowledge of the commonly isolated organisms and their antimicrobial susceptibility patterns within a given hospital assists in the selection of appropriate antimicrobial treatment.
  A.D. Khosravi , S. Seghatoleslami and M. Hashemzadeh
  The present study was conducted to determine the frequency of NTM by application of PCR-based Restriction Fragment Length Polymorphism (PCR-RFLP) among suspected tuberculosis patients. In total 150 clinical isolates from patients referred to TB reference laboratory were screened. Culture and biochemical tests were performed. The PCR-RFLP method based on amplification of a 439 bp fragment of hsp gene involving genus specific primers was performed and the PCR products were digested with HaeIII and Bst EII restriction enzymes. Of total isolates tested, 100 isolates were culture positive (66.6%). Eighty out of 88 isolates that were subjected to RFLP, showed the identical restriction patterns similar to Mycobacterium tuberculosis (90.9%). Eight clinical isolates (9.1%) showed different restriction patterns, six isolates identified as Mycobacterium intracellularae and two isolates were Mycobacterium gordonae I. In conclusion, RFLP as a fast, cheap and accurate technique is a valid alternative for phenotypic identification of pathogenic and potentially pathogenic mycobacteria in the routine laboratory.
 
 
 
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