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Articles by A.B. Ariff
Total Records ( 3 ) for A.B. Ariff
  N.C. Anusha , M.S. Umikalsom , T.C. Ling and A.B. Ariff
  Relationship between fungal growth morphology and the ability to produce lipase in solid state fermentation was studied employing four fungal strains (A. niger DSMZ 2466, A. niger SDTC SRW-4, T. viride SDTC EDF 002 and A. terreus DSMZ 5770). The fermentations were performed in 500 mL flask using palm kernel cake as substrate. Scanning Electron Microscope (SEM) was used to examine growth morphology of the fungi. Pearson correlation and regression analyses performed on quantified morphological dimensions and the fungal growth, enzyme productivity and total protein were able to generate predicted values of equation models. All four strains showed good growth on palm kernel cake at day 6 of fermentation ranging from 0.49 to 0.71 mg g-1 dry substrate. Among these strains, substantial lipase activity was only obtained by A. niger DSMZ 2466 and A. niger SDTC SRW-4 which also showed positive relationship between spore diameter (quadratic, R2; 0.999) and branch diameter (linear, R2; 0.971) with lipase activity. T. viride SDTC EDF 002 and A. terreus DSMZ 5770 showed positive correlations for tip length and tip diameter and size of hyphae diameter and the total protein content in the fermentation substrate. Results from this study showed that the fungi with larger spore diameter and branch diameter contributed to higher ability in producing lipase.
  Z.T. Harith , F.M. Yusoff , M. Shariff and A.B. Ariff
  The aim of this study was to optimize and to propose the suitable separation method and storage conditions for specific species of microalgae. The performance of different separation methods for the recovery of cell biomass of marine microalgae, Chaetoceros calcitrans, from the culture broth was evaluated. The microalgae were cultivated using 10 L photobioreactor. The microalgae cell cultures were concentrated either by centrifugation, tangential flow filtration or flocculation and then stored at different temperatures (-20, 4 and 27°C) to investigate the optimum storage conditions for C. calcitrans prior to different downstream processing methods. High concentration of cell in slurry (4.88x107 cells mL-1) was obtained using centrifugation as compared to tangential flow filtration (4.14x107 cells mL-1), flocculation with chitosan (1.56x107 cells mL-1) and flocculation with Magnafloc®LT 25 (8.24x106 cells mL-1). Storage of C. calcitrans biomass at chilled temperature (4°C) directly after the harvesting using these four different separation methods resulted in extended shelf life (> 4 weeks). Frozen biomass (-20°C) fails to preserve the quality of C. calcitrans after they were revived in fresh medium. C. calcitrans flocculated with 0.5 mg L-1 Magnafloc®LT 25 was able to maintain the quality of the cells after storage at 27°C for more than 2 weeks. However, flocculation of cells with 20 mg L-1 chitosan, centrifugation at 8000 rpm for 10 min and tangential flow filtration process at transmembrane pressure of 20 psi failed to retain the quality of biomass after storage for 2 weeks at 27°C.
  R. Aloysius , M.I.A. Karim and A.B. Ariff
  Studies on the feasibility of using free and immobilized live cells of Rhizopus oligosporus as a biosorbent to remove cadmium from solution was carried out using shake flask experiments. The effect of various conditions such as pH, different initial cadmium concentrations, different biomass concentrations and initial cadmium concentration to biomass concentration ratio was investigated. The biosorption of Cd2+ was determined using several sorption isotherm models such as Langmuir and Scatchard plots. The Langmuir sorption model was found sufficient to describe the biosorption of cadmium by both immobilized and free cells, suggesting that the process was chemical, saturable and equilibrated mechanism similar to ion- exchange mechanism of metals adsorption. A curve of Scatchard transformation plot reflected the covalent nature of Cd2+ adsorption by live cells of Rhizopus oligosporus. Maximum uptake capacity for immobilized cells was about 2-fold higher (34.25 mg/g) than free cells. The immobilized cells projected a higher cadmium uptake capacity with increasing biomass concentration compared to free cells which reached optimum at 0.5 g/L. The initial cadmium concentration to biomass concentration ratio for immobilized cells was lower (33.3 mg/g) compared to free cells (200 mg/g) reflecting that effective removal of Cd2+ can be obtained with increasing immobilized biomass concentration. In bioreactor, the cadmium uptake capacity in comparison with shake flasks experiments for immobilized cells was not effected as observed for free cells. In fixed bed-column, packed-bed with immobilized cells permitted better process control with 2.5-fold higher (0.18 Lh–1) influent feeding rate achieved compared to packed-bed with free cells. About 99 per cent of cadmium removal was achieved for influent containing 5 mg/L and 20 mg/L of cadmium indicating strong affinity of free and immobilized live cells of Rhizopus oligosporus towards Cd2+.
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