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Articles by A.A. Sadeghi
Total Records ( 3 ) for A.A. Sadeghi
  A.R. Seidavi , S.Z. Mirhosseini , M. Shivazad , M. Chamani and A.A. Sadeghi
  In the present study, a multiplex Polymerase Chain Reaction (mPCR)-based assay was developed for the simultaneous identification of Salmonella sp., Escherichia coli and Bifidobacterium sp., that isolated from broiler gastrointestinal in Iran. In order to detect these bacteria species, we were used a set of specific primers that produce different fragment size. Our results showed that all tested gastrointestinal segments were contaminated by these species and therefore the mPCR method is a appropriate method to identify these species directly from poultry gastrointestinal samples. There was no difference in the sensitivity of this method between samples of different gastrointestinal segments. This method has the potential to save considerable time and effort within the laboratory. Our developed mPCR is fast, sensitive, specific and can be used as a reliable choice for routine detection method of these bacteria species in chick gastrointestinal contents.
  H.R. Shahbazi , A.A. Sadeghi , H. Fazaeli , G. Raisali , M. Chamani and P. Shawrang
  The effect of electron beam irradiation at doses of 100, 200 and 300 kGy on ruminal dry matter degradation kinetics of wheat straw was investigated. Samples were irradiated by electron beam irradiator under identical conditions of temperature and humidity. Nylon bags of untreated or irradiated wheat straw were suspended in the rumen of three Taleshi steers for up to 96 h and resulting data were fitted to non-linear degradation model to calculate degradation parameters of dry matter. Results show that the washout fractions of dry matter increased linearly (p<0.001), but potentially degradable fraction and degradation rate decreased linearly (p<0.001) by electron beam irradiation. As a consequence, the effective degradability of dry matter increased linearly (p<0.001) with increasing irradiation dose. Electron beam irradiation at doses of 100, 200 and 300 kGy increased the effective degradability of dry matter at rumen outflow rate of 0.05/h by about 7, 15 and 18%, respectively. In the condition of this study, electron beam irradiation at dose of 200 kGy appeared to be a suitable dose for improving dry matter degradability of wheat straw in the rumen.
  H.R. Shahbazi , A.A. Sadeghi , P. Shawrang and G. Raisali
  The effects of gamma irradiation on ruminal dry matter, Neutral Detergent Fiber (NDF) degradation of alfalfa hay were investigated. Alfalfa hay samples were irradiated by gamma irradiator at doses of 50, 100 and 150 kGy under identical conditions of temperature and humidity. Nylon bags of untreated or irradiated samples were suspended in the rumen of three Taleshi bulls for up to 96 h and resulting data were fitted to non-linear degradation model to calculate degradation parameters. Results indicated that the washout fractions of dry matter and NDF increased linearly (p<0.001) with increasing irradiation dose. The b fraction and the degradation rate of the b fraction (c) of DM and NDF were the highest at 50 kGy dose. Effective degradability of DM and NDF increased linearly with increasing irradiation dose. Gamma irradiation at doses of 50, 100 and 150 kGy increased the effective NDF degradability of alfalfa hay at rumen outflow rate of 0.05 h-1 by about 8, 11 and 12%, respectively. Gamma irradiation affects on the hydrogenic bonds and with theirs breakdowning causes the wander-valls power weaken, that results in the degradation of cellulose and increasing of DM and NDF degradability.
 
 
 
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