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Articles by A.A. Rezk
Total Records ( 3 ) for A.A. Rezk
  A.M. Soliman , B.N. Barsoum , G.G. Mohamed , A.A. Rezk , A.E. Aboul-Ata and H.M. Mazyad
  This study, aims at determination of efficiency of micro interfering RNA (miRNA) to develop ability of virus resistance against Egyptian PVX isolate (PVX-Eg2) in both potato (Solanum tuberosum L. cv. Spunta) and tobacco (Nicotiana benthamiana). RNA constructs of Sense (PVX-Eg2cpVs), antisense (PVX-Eg2cpCs) and sense/antisense were designed, cloned and sub- cloned for gene transfection using Agrobacterium inoculation technique. Two to 3 leaf-stage seedlings of potato (Solanum tuberosum L. cv. Spunta) and tobacco (Nicotiana benthamiana) were inoculated with the three previous constructs. The construct-treated plants were mechanically inoculated with the PVX-Eg2 isolate. Bioassay and PCR amplification have been able to evaluate transfected-plant resistance against PVX-Eg2 that is caused by siRNA of PVX-Eg2cp. PCR amplification has been able to detect PVX viral genome in all challenged plants those infiltrated with either pFGC5491 vector without insert, or with sense construct and also with antisense construct. Bioassay has confirmed same previous statement. Nine out of 10 sense/antisense-transfected potato plants were negatively reacted with both bioassay and PCR amplification. Same negative reaction has been viewed using both bioassay and PCR for sense/antisense transfected-tobacco plants. Seven out of 10 proved they are PVX-Eg2 resistant.
  Kh.A. El-Dougdoug , Rehab A. Dawoud , A.A. Rezk and A.R. Sofy
  Direct tissue printing on membranes has been applied on a large scale for an initial detection of CEVd, HSVd and PLMVd in fruit trees in Egypt. CEVd was detected mainly in sweet orange trees and occasionally in grapevine and mango. The principal characteristics of the disease on sweet orange trees. It was incidence with 15.4, 4.5 and 1.5%, respectively. HSVd was detected mainly in sweet orange trees and occasionally in apple, apricot, mandarin, grapevine, mango, peach, pear and plum trees with 25.2, 2.2, 7.2, 10.5, 12.4, 15.7, 65.6, 40.5 and 5.7%, respectively. The principal characteristics of the disease on sweet orange trees. PLMVd was detected mainly in peach and occasionally in apple, apricot, grapevine, mango, pear and plum with 45.0, 5.4, 2.5, 0.5, 13.5, 23.4 and 3.5% incidence. The principal characteristics of the disease on peach trees. The three viroids; CEVd, HSVd and PLMVd were detected frequently in sweet orange and peach occasionally in grapevine, pear, mango, plum and apricot in Egypt.
  K.A. El-Dougdoug , A.A. Rezk , Dawoud A. Rehab and A.R. Sofy
  Chrysanthemum stunt viroid Egyptian isolate (CSVd-EG) was isolated from infected Chrysanthemum plants. It is a member of Pospiviroidae. In order to study the structure of CSVd-EG, it was reverse transcribed in total RNA from infected leaves and then amplified by polymerase chain reaction (PCR) using Pospiviroid-CCR specific primers. Purified gel RT-PCR product (~199) was cloned into the PCR® II-TOPO® vector then it was sequenced. Partial sequence 199 bp of CSVd-EG is almost identical to that of the prototype 199 bp Canada and USA isolates of CSVd with 96% homology. The sequence of CSVd-EG can be arranged into viroid specific rod like structure. CSVd-EG differ from the prototype isolates Canada and USA at sites occur in regions corresponding to the conserved, variable and right terminal domains which are believed to control viroid pathogencity. Finally, this constitutes the first isolation and identification of CSVd from diseased Chrysanthemum plants in Egypt.
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