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Articles by A.A. Osinubi
Total Records ( 4 ) for A.A. Osinubi
  L.C. Saalu , A.A. Osinubi , P.I. Jewo , A.O. Oyewopo and G.O. Ajayi
  The therapeutic value of Doxorubicin (DOX) as anticancer antibiotic is limited by its organotoxicity. It has been shown that free radicals are involved in doxorubicin-induced toxicity. Doxorubicin causes the generation of free radicals and the induction of oxidative stress, associated with cellular injury. Because of the great importance of DOX in cancer therapy, researchers have expended great efforts trying to prevent or attenuate the side effects of DOX administration. There has, however, been only a little success in this regard. In this study the ameliorating role of antioxidant-rich ethanolic seed extract of Citrus paradisi (CP) on DOX-induced testicular oxidative stress and impaired sperm parameters was investigated. Three experimental groups of Wistar rats were used; CP-alone group that received orally CP 10 mg kg-1 b.wt. daily for 14 days followed by intraperitoneal (i.p.) Normal Saline (NS) 2.5 mg kg-1 b.wt. DOX-alone group that had ip DOX 10 mg kg-1 b.wt. as a single dose. CP plus DOX-group that were similarly given DOX, but also had oral CP 10 mg kg-1 b.wt. pretreatment for 14 days. There was also a corresponding control group. The animals were autopsied 8 weeks after DOX or NS injections. Results showed that DOX-induced reduction in sperm motility and epididymal sperm concentrations as well as increase in total abnormal sperm rates were all normalized in the group pretreated with CP. Pretreatment with CP ameliorated the testicular content of Glutathione (GSH) and Auperoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) activities. Similarly, CP treatment attenuated the DOX-induced increase in testicular lipid peroxidation reflected by malondialdehyde (MDA) levels. These data indicate that CP protects the rat testis against DOX-induced oxidative stress and deranged sperm characteristics.
  L.C. Saalu , G.O. Ajayi , A.A. Adeneye , I.O. Imosemi and A.A. Osinubi
  In the present study, we examined the ameliorating effect of the 100% ethanol extract of Citrus paradisi (grapefruit) seed (GSE) on survival of doxorubicin treated rats and on DOX- induced cardiomyopathy. Whereas only 20% of the rats treated with DOX (20 mg kg-1 body weight intraperitoneally) survived at the end of 14 days, almost all the DOX-treated rats survived when GSE (20 mg kg-1 body weight) was administered by gastric gavage. In the second experiment, GSE (20 mg kg-1 body weight) was administered daily by gavage for 14 consecutive days before a cumulative single dose of DOX (20 mg kg-1 body weight, intraperitoneally) was given. DOX induced marked biochemical alterations characteristic of cardiac toxicity. There was enhanced lipid peroxidation measured as malondialdehyde (MDA). The anthracycline antibiotic drug reduced the cardiac enzymatic activities of superoxide dismutase (SOD), glutathione S transferase (GST) and catalase (CAT). Besides, it reduced significantly the reduced glutathione (GSH) level; prior administration of grapefruit seed extract ahead of doxorubicin challenge ameliorated all these biochemical markers. Taken together, one could conclude that grapefruit seed extract has a protective role in the abatement of doxorubicin-induced cardiac toxicity that resides, at least in part, on its anti-radical effects.
  L.C. Saalu , A.A. Osinubi and J.A. Olagunju
  In the present study, we assessed the early and delayed effects of a single dose of DOX on the testicular oxidative status and spermatogenesis in rats. Forty male adult (11 to 13 weeks old) Wistar rats weighing 185-210 g were used for this research work. The rats were randomly divided into four groups of ten rats each. The control group of rats were given a single dose of normal saline (2.5 mL kg-1) b.wt. intraperitoneally (i.p.) and then were sacrificed two weeks after. Group A animals had 10 mg DOX kg-1 b.wt. i.p. as a single dose. These rats were sacrificed two weeks after DOX administration. Group B animals had 10 mg DOX kg-1 b.wt. i.p. as a single dose but were sacrificed 8 weeks after. Group C rats had similar treatment as those in group B, except that they were sacrificed at the end of the 16th week after DOX administration. Epididymal sperm characteristics were evaluated. In addition, biomarkers of oxidative stress namely testicular antioxidants and lipid peroxidation in the testicular tissue were determined using spectrophotometric methods. The testicular oxidative status of DOX-treated rats was severely compromised as evidenced by the significant decrease in the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), in addition to the significant reduction in the reduced glutathione (GSH) level as well as the significantly enhanced lipid peroxidation measured as malondialdehyde (MDA). There was also a demonstratable worsening of the rat testicular oxidative status and sperm parameters with passage of time following DOX administration. The results indicate that DOX produces persistent damage to the spermatogenic compartment of the testis as well progressive increase in the testicular oxidative stress.
  L.C. Saalu , A.A. Osinubi , J.A. Oguntola , I.O. Adeneye and A.S. Benebo
  Doxorubicin (DOX), one of the anthracycline antibiotic drugs isolated from the soil fungus Streptomyces peucetius caesius, which has been widely used to treat cancer effectively. It has also been known to induce reproductive abnormalities in males. The implication of natural phenolic compounds in the prevention of many pathologic diseases has been reported. Herein, the ability of polyphenolic-rich Grapefruit Seed Extracts (GSE), to protect rat testis against DOX-induced histomorphometric impairment was investigated. Four groups of Wistar rats were used; The GSE- alone group received intraperitoneal (i.p.) Normal Saline (NS) 2.5 mg kg-1 b.wt. followed by orally GSE 10 mg kg-1 b.wt. daily for 16 weeks. The DOX-alone group were given i.p., DOX 20 mg kg-1 b.wt. as a single dose. The GSE plus DOX-group were similarly given DOX, but also had oral GSE 10 mg kg-1 b.wt. post-treatment for 16 weeks. Another group of rats were each given orally 2.5 mL kg-1 b.wt. peanut oil (vehicle) daily for 16 weeks, after 2.5 mL kg-1 b.wt. normal saline was given as a single dose i.p., to serve as the control. The animals were sacrificed 16 weeks after DOX or NS injections. The testicular toxicity induced by DOX was assessed by histologic and stereologic evaluation of the testis. Present results demonstrated that post-treatment with GSE was capable of reversing the reduction of body and testicular weights as well as the testicular histomorphometric evidences of high dose and delayed DOX toxicity in the animals. The GSE was therefore shown to exert testicular cytorejuvinative effects on rats challenged with DOX.
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