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Articles by A.A. Mousa
Total Records ( 4 ) for A.A. Mousa
  Kh.A. El-Dougdoug , S.A. Ghazal , A.A. Mousa , H. Fahmy and A.R. Sofy
  This study was aimed to determined PCR product of the CP gene by electrophoresis. Three Egyptian isolates of Citrus psorosis virus (CPsV-EG), namely ARC, TB and TN were obtained from citrus cvs. Grapefruit, Balady and Navel, respectively. These isolates were differed in some of their external symptoms. The CPsV-EG isolates were detected by biological indexing, giving rise to Oak Leaf Pattern (OLP) on Dweet tangor. The three isolates were differentiated using Double Antibody Sandwich-Enzyme-Linked Immunosorbent Assay (DAS-ELISA), woody indicator plants, differential hosts, peroxidase isozymes and activity, total RNA content and Reserves Transcription-Polymerase Chain Reaction (RT-PCR). The severe isolate (ARC) gave the highest OD value (2.204) in ELISA, followed by the mild isolate (TB) (1.958) and the last latent isolate (TN) (1.669). These isolates differed also in incubation period, intensity of symptoms and response to sensitivity of woody indicator plants and differential hosts. The CPsV-EG isolates showed differences in isozymes fractions, RF value and intensity as compared with healthy plant. Results were confirmed by peroxidase activity where the level of peroxidase activity was considerably higher in ARC leaves than TB and the last TN. The total RNA content in infected leaves gave the highest content in ARC followed by TB isolate while the lowest was recorded in TN isolate. Finally, RT-PCR showed differences between CPsV-EG isolates of PCR products using specific primer (Ps66 and Ps65) where base number of coat protein gene ARC isolate 571 bp; TB isolate 529 bp and TN isolate 546 bp.
  A.R. Sofy , A.M. Soliman , A.A. Mousa and Kh.A. El-Dougdoug
  This study reports the characterization of Hop stunt viroid which has been isolated previously in Egypt from sweet orange infected with gummy bark disease namely citrus viroid II. The native structure of mature, circular forms of the gummy bark agent was detected by gel electrophoresis. The expected size of amplified cDNA by RT-PCR was approximately 300 bp. A phylogenetic tree of the Egyptian citrus gummy bark agent (Accession no. FJ984562) revealed 100, 99.7 and 97.8% a moderate degree of similarity to CVd-IIb (USA), CCaVd (Spain) and CCaVd (Egypt), respectively. The minimum free energy of a secondary structure for HSVd-EG-RNA was determined using its primary sequence at 37°C. The sequence appeared to fold into a rod-like structure at -122.1 kcal moL-1 while CCaVd-EG at -120.5 kcal moL-1. The five domains of the rod-like structure were determined. The sequence variations between Egyptian citrus gummy bark isolate and Egyptian citrus cachexia isolate in the pathogenic domain (P) tend to influence the pathogenicity of the HSVd-EG. Finally, the genetic diversity and evaluation of entropy power for the Egyptian citrus gummy bark agent and HSVd-citrus populations registered in GenBank, were viewed against the phylogenetic background of known CVd-II variants including the non-cachexia (CVd-IIa) and the causal agents of severe (CVd-IIb, CVd-IIc), more moderate (Ca903) and mild (Ca909).
  A.R. Sofy , A.A. Mousa , A.M. Soliman and Kh.A. El- Dougdoug
  Gummy bark disease is a disorder of sweet orange on sour orange rootstock in Egypt. There is an importance for hot-growing temperatures to symptom development of citrus viroids. The geographical distribution of the gummy bark disease in some world countries depend on high temperatures for viroid-symptom expression. So, correlation between climatic factors and gummy bark disease through GIS is studied. We designed a satellite map for the gummy bark disease distribution all over the world using the previous registered results. Superimposed maps of BIOCLIM annual Min-temperature, Max-Temperature and the points distribution, indicated that gummy bark disease naturally occurs in the low temperature zones range from 8-18°C at winter and from 27-38°C at summer season where the altitude ranged from -351 to 1320 m. A novel method called maximum entropy distribution modeling was used for predicting potential suitable habitat for gummy bark disease in Egypt using occurrence records. The Maxent model’s internal jackknife test of variable importance showed that altitude and mean temperature of driest quarter are the most important predictors of citrus gummy bark disease-habitat distribution.
  Taghreed A. Hassan and A.A. Mousa
  In this study, the stochastic production planning involving random variable coefficients and random demand is optimized using an interactive stochastic approach based on reference point satisficing method. This approach combines the concept of probability efficiency for stochastic problems with the reference point method for deterministic multiobjective problems. The decision maker expresses her/his references of each objective and by setting the desired probability for each objective to achieve values belonging to each reference. The proposed approach enable the DM to learn in depth the features of the problem, to evaluate the consequences of each decision and to know the trade-offs between the levels and the probabilities inside each objective function and also among them.
 
 
 
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