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Articles by A.A. Hamid
Total Records ( 7 ) for A.A. Hamid
  H.M. Ibrahim , W.M.W. Yusoff , A.A. Hamid and O. Omar
  The objective of this study was to enhance the production of Cyclodextrin glucanotransferase (CGTase) produced by a local isolate Bacillus G1. CGTase produced in an optimized medium using different fermentation modes was investigated. The performance of batch mode, continuous mode and fed-batch mode was achieved in 5 L-stirred tank fermenter. Maximum CGTase production (77.49 U mL-1) was achieved in fed-batch fermentation with 23.7% improvement compared to batch fermentation (62.63) and 16.6% improvement compared to continuous fermentation (66.47). CGTase productivity in fed-batch fermentation (1.6 U/mL/h) improved by 14.3% compared to that obtained by continuous fermentation (1.4 U/mL/h) and 23.1% improvement compared to batch fermentation (1.3 U/mL/h).
  N.K.N. Al-Shorgani , M.S. Kalil , E. Ali , W.M.W. Yusoff and A.A. Hamid
  Butanol is an important industrial chemical and has gained attention as an important fuel because of its advantages of being less corrosive and water tolerant as compared to the ethanol. This study revealed the effects of butyric acid as an additive on growth and Acetone-butanol-ethanol (ABE) production using batch culture of Clostridium saccharoperbutylacetonicum N1-4. Different combinations of glucose and butyric acid were studied to finalize the best productive ratio for ABE and butanol production. The highest ABE and butanol production was obtained when 4 g L-1 of butyric acid was used in the presence of 30 g L-1 of glucose. The inhibitory effects of butyric acid on bacterial growth were also investigated using C. saccharoperbutylacetonicum N1-4 and mild inhibitory effects were found at high butyric acid concentration. On the other hand, no linear correlation between butyric acid and butanol production was observed. Production of 17.76 g L-1 butanol with a productivity of 0.15 g L-1 h-1 from 4 g L-1 of butyric acid proved the ability of C. saccharoperbutylacetonicum N1-4 to be tolerant to the certain concentration of butyric acid for the enhanced butanol production. Butyric acid was not only contributing as an additive or stimulating agent to the butanol pathway but also was being utilized as a co-substrate. Enhanced butanol production using growing cells of C. saccharoperbutylacetonicum N1-4 in the presence of specific concentration of butyric acid (4 g L-1 butyric acid) as a co-substrate with glucose can be carried out without any remarkable inhibition to bacterial growth.
  H. Alshiyab , M.S. Kalil , A.A. Hamid and W.M.W. Yusoff
  The aim of this study was to investigate the influence of some environmental factors on bacterial metabolism. Fermentative hydrogen production by C. acetobutylicum, using glucose as the substrate. The effect of initial pH (4-8), inoculum size (1-20% (v/v)) and glucose concentration (1-30 g L-1) on hydrogen production were studied. The optimum cultivation temperature for hydrogen production was at 30 °C. The results show that substrate concentration and inoculum size resulted in hydrogen yield (YP/S) of 391 mL g-1 glucose utilized with maximum hydrogen productivity of 77.5 mL/L/h. Higher substrate concentration or inoculum size adversely affects hydrogen production, which decreases hydrogen yield by 15% to 334 mL g-1 glucose utilized when 30% (v/v) inoculum size was used. The use of 30 g L-1 substrate concentration resulted in a 75% decrease to 97 mL g-1 glucose supplied. Concluded that proper Xo/So enhanced the hydrogen production.
  H. Alshiyab , M.S. Kalil , A.A. Hamid and W.M. Wan Yusoff
  The objective of this study is to investigate the effect of salts addition to fermentation medium on hydrogen production, under anaerobic batch culture system. In this study, batch experiments were conducted to investigate the inhibitory effect of both NaCl and sodium acetate on hydrogen production. The optimum pH and temperature for hydrogen production were at initial pH of 7.0 and 30°C. Enhanced production of hydrogen, using glucose as substrate was achieved. In the absence of Sodium Chloride and Sodium Acetate enhanced hydrogen yield (YP/S) from 350 mL g-1 glucose utilized to 391 mL g-1 glucose utilized with maximum hydrogen productivity of 77.5 ml/L/h. Results also show that sodium chloride and sodium acetate in the medium adversely affect growth. Hydrogen yield per biomass (YP/X) of 254 ml/L/g, biomass per substrate utilized (YX/S) of 0.268 and (YH2/S) of 0.0349. The results suggested that Sodium at any concentration resulted to inhibit the bacterial productivity of hydrogen.
  A.M. Mukred , A.A. Hamid , A. Hamzah and W.M. Wan Yusoff
  Addition of nitrogen sources as supplementary nutrient into MSM medium to enhance biodegradation by stimulating the growth four isolates, Acinetobacter faecalis, Staphylococcus sp., Pseudomonas putida and Neisseria elongata isolated from petroleum contaminated groundwater, wastewater aeration pond and biopond at the oil refinery Terengganu Malaysia was investigated. The organic nitrogen sources tested not only supported growth but also enhances biodegradation of 1% Tapis crude oil. All four isolates showed good growth especially when peptone was employed as the organic nitrogen compared to growth in the basal medium. Gas chromatography showed that more then 91, 93, 94 and 95% degradation of total hydrocarbon was observed after 5 days of incubation by isolates Pseudomonas putida, Neisseria elongate, Acinetobacter faecalis and Staphylococcus sp., respectively.
  H. Alshiyab , M.S. Kalil , A.A. Hamid and W.M.W. Yusoff
  The effect of removal of resultant gas resulted in enhancement of the H2 yield. The technique of CO2 scavenging resulted in H2 yield being improved from 408 mL g-1 to reach the maximum of 422 mL g-1. The highest hydrogen productivity of 87.9 ml L-1 h-1 was obtained by CO2 scavenging. Biomass concentration was enhanced to 1.47 g L-1, YP/X of 287 ml g-1 L-1, YX/S of 0.294 and YH2/s of 0.0377 by the use of CO2 scavenging. The results suggested that the presence of the gaseous products in fermentation medium and headspace adversely effect biomass growth and hydrogen production.
  Noura, K.M. Salih , N.H. Jusuf , A.A. Hamid and W.M.W. Yusoff
  In the present study, Ten soil samples were examined and the pH of the soil was recorded. For bacterial isolation, a sterile nutrient and blood agars were used. Gram stain and biochemical tests were done for identification. A total of 384 genus were isolated, 314 (81.8%) were identified as Pseudomonas species of which 245 (78.0%) were Pseudomonas aeruginosa, 42 (13.4%) were Pseudomonas fluorescens, 13 (4.2%) were Pseudomonas mallei, 10 (3.1%) were Pseudomonas putida and 4 (1.3%) were Pseudomonas syringe and are regarded as pathogenic and harmful to man, animal and plants. This study shows that Pseudomonas aeruginosa had a high adaptation capability to grow in soil samples from Ternate, Indonesia. The rest of the bacterial isolates (18.2%) were identified as follows: 24 samples (6.2%) were Micrococcus, 23 samples (6.0%) were E. coli, 12 samples (3.1%) were Pasteurella and 11 samples (2.9%) were Staphylococcus. Pencillium was also isolated.
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