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Articles by A.A. El-Sanousi
Total Records ( 2 ) for A.A. El-Sanousi
  H.A. Hussein , H.A. Sultan , A.H. EL-Deeb and A.A. El-Sanousi
  Re-emerging of H5N1 severe outbreaks in vaccinated chickens housed in poultry farms in Sharkia governorate in Egypt was observed starting from October 2007 and continued for 3 months until control measures have been taken in such farms, besides multiple vaccination during this period were applied using reassortant H5N1 and 6 types of H5N2 vaccines. In the present study, 9922 serum samples were collected from vaccinated chickens including some broiler breeders and commercial layers from the period of December 2006 to February 2008 and tested for the immune response to the multiple vaccination with different H5N1 and H5N2 avian influenza vaccines (reassortant H5N1 and 6 types of H5N2 vaccines) by Hemagglutination Inhibition (HI) test using heterologous H5 antigen which represent non of the vaccine antigens. The samples were collected from 578 houses mostly commercial layers. H5N1 viral nucleic acid was also detected in swab samples collected from mortalities occurred in some of vaccinated birds after vaccination policy has been applied in Egypt at March 2006. The viral RNAs of the detected H5N1 circulating viruses between Feb. 2006 and Feb. 2008 in Sharkia governorate were sequenced. Results of nucleotide sequence analysis of 11 detected viruses confirm the existence and circulation of the H5N1 in Sharkia governorate along the period of study and no H7 viruses were detected. The serum samples collected from different farms were divided into 4 groups based on the date of collection. Hemagglutination Inhibition (HI )results showed that in the first group (samples collected between December 2006 to March 2007) 43% of the tested samples were of HI titers of 6 log 2 or more which we propose such titer as a protective titer against H5N1 virus. In group 2 (samples collected between April and July, 2007), titers in 36.4% of the tested samples were protective. In group 3 (samples collected between August to November, 2007), titers in 33.2% of the samples were protective. The last group contains samples collected from chicken vaccinated twice during the period of collection (December 2007 to February 2008), besides being previously vaccinated with at least 3 vaccine shots. Hemagglutination Inhibition (HI) titers in such group were protective in 54% of the tested samples although these chickens received 5 doses of H5 vaccines. The present study is highlight the possible multiple causes of re-emergence of H5N1 outbreaks in Egypt.
  N.M. Hagag , A. Arafa , M.A. Shalaby , A.A. El-Sanousi and M.M. Aly
  Highly Pathogenic Avian Influenza (HPAI) caused by influenza A H5N1 virus, poses a significant threat to the poultry industry and humans in Egypt. Since it was first recognized in 2006, the disease has become enzootic in poultry throughout Egypt and still circulates in the poultry population, so the ability to rapidly recognize AIVs in biological specimens is critical for limiting further spread of the disease in poultry. Application of molecular methods such as Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and Real time RT-PCR (RRT-PCR) as a rapid, specific and sensitive detection methods currently used in national and reference laboratories worldwide. In this study a comparison of the specificity and sensitivity between 2 different formats of conventional RT-PCR (one and two steps) and 3 different formats of RRT-PCR (one step using TaqMan® probe, two steps using TaqMan® probe and two steps using hybridization® probe) were performed and compared as a diagnostic tools for H5N1 virus detection. All these formats of PCRs appeared within the same specificity for H5 gene detection, while they showed difference in sensitivity as the one step conventional RT-PCR showed to be more sensitive than two steps conventional RT-PCR by 10 folds, one step RRT-PCR TaqMan® probe was more sensitive than two steps RRT-PCR TaqMan® probe by 10 folds, two steps RRT-PCR hyberidization® probe is more sensitive than two steps RRT-PCR TaqMan® probe by 100 folds, finally two step RRT-PCR using hyperidization® probe is more sensitive than conventional RT-PCR two steps by 1000 folds. Fifty one field samples were further tested by all mentioned PCR formats the results were the same of that obtained in the sensitivity experiment and agreed with them in placing hybridization probe system of higher sensitivity than TaqMan one step than TaqMan two steps and conventional PCR were of the lowest sensitivity although one step showed higher sensitivity than two steps.
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