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Articles by A. Zakeri
Total Records ( 4 ) for A. Zakeri
  M. Keykhahsaber , A.A.V. Sedehi , A. Zakeri , H. Khaje and Z. Keykhah
  In order to determine the level of low temperature response genes expression in ten selected barley accessions were obtained from Sistan Agricultural Research at spring of 2008. RNA from each of barley germination was extracted in two cold acclimation and one control condition. The result of real time PCR, using cDNA from three low temperature response gene (bit2, bit14 and bit101), showed that there were significant difference in gene expression between three treatments and in each gene the highest percentage of gene expression belonged to accession 5 while accession 8, 9 and 10 composed one separate. In bit14 gene, the increase in the amount of mRNA was carried out when the maximum level of freezing (4 °C day/2 °C night) apply. All of these genes are shown to be transcriptional regulated and root meristem had maximum level of RNA under cold treatment.
  M. Farahbakhsh , S. Fozounkhah , H. Sadeghi-Bazargani , A. Zakeri , N. Houshiyan , N. Asmani and A. Naghili
  Human resources comprise an important element in implementation of any successful health information system including any kind of information technology used. Our aim of study was to investigate some aspects of the current health information system in Tabraiz University of Medical Sciences and its different subdivisions and study attitudes of health managers and health experts regarding this system. In this cross-sectional study a total of 240 health system experts along with 32 health system managers working in different subdivisions of Tabriz University of Medical Sciences and Health Services through out the province were entered into the study. A structured questionnaire was used to collect the main data and some extra data were gathered checking the registers and available files. Data were entered into the computer and analyzed with SPSS 11 statistical package. We found in this study that Excel and Access were the main statistical software packages installed and used in information and statistics units. Data collection was commonly done in 71.2% of information and statistics units based on a given standard plan. The main procedures used for analyzing or interpreting the data were comparing with previous data in 59.4%. Planning and decision making were the most common purposes of using information as declared both by managers and experts. 61.9% of managers compared to 47.5% of health system experts said they commonly use available statistical data to evaluate services supplied by their own units. Nearly a quarter of both managers and health system experts said they commonly receive feedback from higher levels of organization regarding the statistical information sent by them.
  A. Zakeri and P. Kashefi
  Antimicrobial agents are used extremely in order to reducing the enormous losses caused by Escherichia coli infections (Colibacillosis) in Iran poultry industry. In this investigation, 200 Avian Pathogenic Escherichia coli (APEC) strains isolated from broiler chickens with Colisepticemia and examined for susceptibility to antimicrobials of veterinary and human significance. Multiple resistances to antibiotics were observed in all the isolates. The highest rate of resistance was against Nalidixic acid (98), Lincomycin (97.5), Erythromycin (97), Oxytetracycline (92), Chloretracycline (92), Flumequine (90), Doxycycline (80), Difloxacin (80), Neomycin (62), Streptomycin (62), Trimethoprim-Sulphamethoxazonle (60), Kanamycin (60), Enrofloxacin (60), Norfloxacin (55), Ciprofloxacin (50), Chloramphenicol (49), Furazolidone (45.5) and Nitrofurantoin (45%). Resistance to Gentamicin, Ceftifur and Fosbac were not observed and to Amikacin, Cefazolin, Colistin, Lincospectin and also Florfenicol were low. This study showed resistance rate against the antibiotics that are commonly used in poultry is very high but against them that are only used in human or less frequently used in poultry is significantly low. This study also showed that the prevalence of Quinolone Resistant Escherichia coli (QREC) are very high in broiler farms in Tabriz province. The high presence of OREC from broiler chickens probably id due to overuse of enrofloxacin in these farms for therapeutic purposes.
  A. Zakeri and P. Kashefi
  Infectious Bursal disease is one of the important diseases of poultry. This disease caused severe economical losses such as mortality, retardation of growth and immunosuppressant, especially at 3-6 weeks chickens. Histopathologic lesions were appeared in Cecal tonsil and other lymphoid tissues. Destruction of infected lymphocytes with virus and peripheral cells and depletion of lymphocytes in Cecal tonsil were caused by necrosis and apoptosis. In this study, 50 SPF 28 days olds chickens were divided in to tow groups (control and experimental) with 25 chickens in each group. The experimental group was infected orally by 106EID50 in 1 mL of IR499 (IRAN499) virus (vvIBDV) in control group physiological saline solution was used. At 4th days post infection, all birds were sacrificed and their Cecal tonsil were taken out and prepared for EM (Electronic Microscope) assay and light microscopic study. By light microscopic study about numeration of apoptotic cells, statistic difference were appeared (p = 0.000) between control and experimental groups. With EM, apoptotic cells were appeared by submargination of chromatin of nuclear membrane concurrent chromatin condensation in experimental group but there were not any apoptotic cells in control group. Apoptosis was appeared by attachment of virus to IgM+ receptors of LB surface and to enforce of cells to secretion of some cytokines. VP2 and 17KD were major viral proteins induced apoptosis in bursa and spleen in infected chickens. In this study and previous studies were demonstrated that IBDV (Infectious Bursal Disease Virus) affected chickens with both of necrosis and apoptosis.
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