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Articles by A. Pandey
Total Records ( 4 ) for A. Pandey
  A. Pandey , M. Kamle , L.P. Yadava , M. Muthukumar , P. Kumar , V. Gupta , M. Ashfaque and B.K. Pandey
  In the context of the GM food regulations crop improvement via transgenic technology is a new stage of introducing novel food which supercedes over the conventional breeding. It was analyzed that worlds hunger, malnutrition problems, environmental pollution and phytoremediation in agriculture are the challenges for scientist as well as governments those can be combated by application of genetic engineering in crops. Genetically modified microbes/plant/animals or GM microbes/plant/animals results from modification in the genetic make-up of microorganisms, plants and animals using recombinant DNA technology to improve the nutritional requirement, disease resistant traits, increased production and medicinal properties. In many instances, these modification processes represent faster, more efficient mechanisms for achieving changes than traditional breeding. However, a wide variety of modifications are possible through genetic manipulation and the potential for the introduction of toxic compounds, unexpected secondary effects and changes in nutritional and toxicological characteristics may give rise to safety concerns about GM crops. Thus, generation of GM food explores new vistas for future food requirement but the assessment of policy regarding environmental risks is also to be concerned.
  A. Pandey , M. Kamle , L.P. Yadava , M. Muthukumar , P. Kumar , V. Gupta , M. Ashfaque and B.K. Pandey
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  A. Pandey , B.K. Pandey , M. Muthukumar , L.P. Yadava and U.K. Chauhan
  Anthracnose, caused by Colletotrichum gloeosporioides, is a serious postharvest disease of mango. The histopathological studies on anatomy of naturally infected by Colletotrichum gloeosporioides and artificially inoculated leaves and healthy leaves were performed to understand the infection process of anthracnose at various intervals after inoculation. Germination and penetration processes of the pathogen within the whole leaf were observed. The first evidence of penetration into the whole leaf was observed 48 h after invasion. It also revealed that mycelia were prominent after 120 h after invasion by the fungus (C. gloeosporioides). Subcuticular infection by hyphae was present in transverse leaf sections (T.S.) of the diseased sample after 72 h. Also, both inter and intra-cellular hyphal invasion were observed after 72 h. Mesophyll cells were highly affected by fungal invasion and rapidly collapsed. Swelling of epidermal cell walls was also observed. After 96 h almost all the cells became necrotized (Nc). Necrotized mycelial mats (M) of C. gloeosporioides was observed after 120 h and all the invaded cells became necrotized (Nc) forming a spot which eventually the cells ruptured leaving a shot hole symptom. All these observations pertained to the cells of mesophyll tissue indicating that these are the regions of fungal invasion and host tissue damage resulting in the disease symptoms. Naturally infected and artificially inoculated (in vitro) presented no significant differences suggesting that the pathogen invasion and symptom development process is similar in both the conditions.
  A. Ghildiyal and A. Pandey
  Three species of Trichoderma viz., T. harzianum, T. konengii and T. viride have been isolated from the soil samples collected from forest sites in higher altitudes of Indian Himalayan Region. The species could grow between 9 to 35°C temperature and 4 to 12 pH on agar plates; the optimum requirement being 24°C and 5.5 pH, respectively. Further incubation of the agar plates showing normal growth of Trichoderma sp. at 4°C, induced heavy sporulation in three weeks of time. Induction of sporulation on exposure to low temperature appeared to be a strategy for survival of these species in extreme cold environment experiencing sub zero temperatures. Antifungal activities were demonstrated between Trichoderma sp. and phytopathogenic fungi in dual cultures. The antifungal metabolites produced by Trichoderma sp., diffusible as well as volatile, caused abnormalities in fungal structures of pathogenic fungi. Plant growth promotion abilities of Trichoderma sp. was also demonstrated through a plant based bioassay in greenhouse. The study is important for documentation of microbial diversity of Indian Himalayan Region (IHR) and determination of the associated biotechnological applications.
 
 
 
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