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Articles by A. Nazemi
Total Records ( 2 ) for A. Nazemi
  Saeed Gholamian , Soudabeh Gholamian , A. Nazemi and M. Miri Nargesi
  L-asparaginase is an anti-neoplastic agent that has been particularly studied for its therapeutic aspects in the treatment of certain types of cancer, especially lymphoblastic leukemia. This study performed screening of soil microbial isolates that were potential producers of extracellular L-asparaginase. The isolates were mainly characterized using both biochemical and molecular approaches. Ninety-six of all L-asparaginase producer bacteria isolated from southern boundaries of the Caspian Sea, were found to belong to Bacillus sp. Among them, Bacillus sp. GH5 exhibited a distinctly high asparaginase activity and therefore underwent further analyses. This study specifically details the results of analyses performed on the mentioned isolate. After incubation at 80°C for 10 min, plate assay was performed on modified M9 medium supplemented with phenol red. Bacillus sp. GH5 showed a significant pink zone. L-asparaginase activity was assayed using direct Nesslerization method showing approx. 300 IU at 37°C. Molecular identification was carried out using the bacterial universal primer set of BAc08F and Uni1390R. Phylogenetic analysis of the 16S rRNA gene revealed that this isolate was a member of the genus Bacillus. Furthermore, effects of temperature and pH on both bacterial grow and enzyme activity was investigated and showed that maximum growth occurred at 37°C and pH 7. In addition, maximum asparaginase activity was observed at 37°C and pH 8.5. Accordingly, the results of this study suggest that Bacillus sp. GH5 is a promising candidate for treatment of leukemia considering its great enzyme activity and particular optimal conditions.
  A. Nazemi , L.T. Shui and M.H. Davoudi
  Recently pervious rockfill detention dam is used as a flood mitigating structure. Analysis of hydraulics of turbulent flow through this kind of dam is mostly done using Darcy-Weisbach equation. So far, many attempts have been made to study the friction coefficient as a function of Reynolds Number in turbulent flow of clean water through pervious rockfill detention dams, while this subject has remained intact for sediment laden flow of water. In this paper relationship between friction coefficient and Reynolds Number for sediment laden flow through highly pervious rockfill dams was investigated. Required data for a regression analysis obtained by conducting a series of laboratory tests to calibrate and validate a proposed power law friction coefficient-Reynolds Number relationship. A changeable bed slop Plexiglas flume, an adjustable rate sediment feeder and a recirculating flow electro pump system were used in present study. The tests were carried out on four different rectangular laboratory rockfill dams and three different non-cohesive suspended sediments. A power law relationship was obtained with a correlation coefficient of 0.74 using two thirds of laboratory measured friction coefficient and Reynolds Number. The obtained relationship was validated employing the remaining unused data with a Mean Square Error of 0.29 which is an acceptable agreement. A new power law relationship was found between friction coefficient and Reynolds Number in sediment laden flow through pervious rockfill dams. This new relationship is the only one thus has been proposed for the sediment laden flow of water through pervious rockfill dams.
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