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Articles by A. Mehrvar
Total Records ( 3 ) for A. Mehrvar
  A. Mehrvar , R.J. Rabindra , K. Veenakumari and G.B. Narabenchi
  Experiments conducted to study the effect of various adjuvants on the larval mortality of Helicoverpa armigera NPV under simulated sunlight revealed that all the adjuvants (either alone or in combination) resulted in significantly higher larval mortality as compared to the virus alone. A combination of three adjuvants, viz., molasses (5.0%) + Tinopal (0.2%) + lampblack (0.1%) showed the highest larval mortality (94.2%) under simulated sunlight with the lowest LT50 values (99.6 h). It was also observed that the persistence of HearNPV was higher when molasses (5.0%) + Tinopal (0.2%) + lampblack (0.1%) was used as adjuvant with an OAR (original activity remaining) of 86 and 36.2% after 1 and 6 days after treatment, respectively under open weather conditions on tomato plants.
  A. Mehrvar
  An experiment was conducted to evaluate the persistence of Helicoverpa armigera NPV after 6 months of storage in two types of soil viz., black soil and red soil under different conditions to screen the relatively persistent HearNPV geographical isolate. The isolates of the virus used in this study were CMB (Coimbatore), NGM (Negamum) and OTY (Ooty) all collected from Tamil Nadu, India. Two types of soils viz., black soil and red soil were selected to study the persistence of HearNPV. Each virus isolate (1x109 OB mL-1) was added to 2 kg of each soil and kept in indoor and outdoor conditions in plastic troughs for 6 months. Assays were carried out with extracted viruses from each soil at monthly intervals against early second instar larvae of H. armigera. Results indicated that as the period of storage of virus in soil was advanced the mean mortality percentage declined. Also, the comparison of results showed that the virus treated black soil kept indoor could relatively retain its infectivity (52.66, 58.56 and 54.35% OAR with CMB, NGM and OTY isolates, respectively) longer period compared to the soil kept outdoor. Similar kind of trend was also noticed with red soil. However, the infectivity was relatively more in the case of HearNPV stored in red soil compared to those stored in black soil. This high amount of persistence in red soil may be due to the fact that adsorption rate was higher in red soil than black soil. However, among the isolates evaluated in this study, NGM isolate was found to be the most relatively tolerant to inactivation through the storage time.
  A. Mehrvar , R.J. Rabindra , K. Veenakumari and G.B. Narabenchi
  The effect of four major parameters, viz., larval age and weight, inoculation dose, incubation temperature and time of harvesting the larvae, on the production of three isolates of nucleopolyhedrovirus of Helicoverpa armigera (HaNPV), viz., Ooty (OTY), Coimbatore (CMB) and Negamum (NGM) were evaluated. Early 5th instar larvae recorded the maximum yield of virus per larva when inoculated with a dose of 5χ105 POB larva‾1 and incubated at a temperature of 25°C. Also, highest POB yield was recorded when virosed larvae were harvested as cadavers. However, among the isolates tested in this study, CMB isolate collected from Tamil Nadu, India, showed the highest yield per larva in all of the conditions.
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