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Articles by A. Makkiya
Total Records ( 2 ) for A. Makkiya
  N. Saleh , M.A. Ibrahim , E. Archoukieh , A. Makkiya , M. Al-Obaidi and H. Alobydi
  The aim of this study is to ascertain the possible application of Random Amplification of Polymorphic DNA (RAPD) analysis as a genetic test to investigate DNA polymorphisms and detection of genomic markers in various types of leukemia. The results showed unique profiles of amplified DNA fragments produced in genomic DNA of three types of leukemia by an arbitrary primer of decamer oligonucleotides OPA-09. The primer produced four types of amplified DNA fragments (980, 1659, 2187 and 3162 bp). The smallest amplified DNA fragment (980 bp) appeared in 14.3 and 13.3% of tested acute myeloid leukemia and chronic myeloid leukemia patients, respectively; but was absent in genomic DNA of chronic lymphoid leukemia and normal individuals. Whereas the largest amplified fragment (3162 bp) was present in 12.5, 20 and 75% of chronic lymphoid leukemia, chronic myeloid leukemia and normal individuals, respectively and was absent in acute myeloid leukemia. On the other hand, the two amplified fragments (1659 and 2187 bp) were present in normal and leukemia patients. Cluster analysis of amplified DNA fragments grouped the leukemia patients in two main groups. The detected DNA polymorphisms by the arbitrary primer OPA-09 might find application in developing efficient RAPD primer for diagnosis of leukemia.
  A. Makkiya , M.A. Ibrahim , N. Al-Hmoud , H.H. Hasani and H.M. Said
  Acute Myeloid Leukemia (AML) is the most frequent cause of acute leukemia affecting adults, its incidence increases steadily with age and has been correlated with DNA methylation aberration and inactivation of tumor suppressor genes. Recent epigenomic studies showed that DNA methylation abnormalities have been observed in age related acute myeloid leukemia and indicated the importance of DNA methylation analysis for AML diagnosis. An insight of the connection between DNA methylation and AML might help in development of novel molecular diagnostic and genomic therapies. Epigenetic drugs of two families namely the DNA-demethylating agents and inhibitors of histone deacetylase have emerged as the most promising compounds in this area and several pharmaceutical compounds have received approval for the treatment of specific leukaemia and lymphoma subtypes. In addition possible combination between molecular therapeutic approaches including the activation of certain signal transduction pathway(s) like the interleukins family and chromatin-remodeling events is feasible by the application of epigenetic drugs. This approach might be one of the potential promising solutions for the AML treatment. The present study reviewed recent advances in the research related to genomic and epigenomic of acute myeloid leukemia.
 
 
 
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