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Articles by A. Latif
Total Records ( 2 ) for A. Latif
  H. Das , S.U. Ahmed , S.K. Shukla , S. Shukla , A. Latif and D. Sharma
  Mastitis represents a failure of innate immune mechanism of udder teat canal epithelial cells and peripheral PMN cells, whose cationic peptides constituted innate immunity. An attempt was made to examine in the year 2007, the actual presence of β-defensins peptides in milk of mastitis-affected buffalo (Bubalus bubalis), due to lack of such information. AU-PAGE revealed higher frequency and density of the peptides in case of mastitis milk as compared to normal milk sample, particularly in the most cationic zone with highest electrophoretic mobility or lower molecular weight region. These low molecular peptides were also separated out using 10 kDa cut-off membrane ultra filtration, which was subsequently examined for in vitro antibacterial sensitivity and subjected to SDS-PAGE and low molecular weight zone was further subjected to MALDI-TOF mass spectrometry, which identified the most anodic two peptides of about 5 kDa as β-defensin viz. The LAP (lingual antibiotic peptide) and BNBD-2 (bovine neutrophil beta-defensin-2) based on amino acid sequences unlike that of healthy buffalo milk. Sequence homology studies also supported mass spectrometry data as both the peptides shared 100 and 98.5% identities with cattle β-defensins, respectively. Antibacterial assay against S. aureus and E. coli revealed significant activity of these peptides against both gram-positive and gram-negative organisms at 10 μg concentration. It is concluded that expression of β-defensin peptides in ductal epithelium were induced in mastitis and these defensin peptides were also released into the milk.
  Fahad N. Al-Majhdi , A. Al-Jarallah , M. Elaeed , A. Latif , L. Gissmann and Haitham M. Amer
  In the current study, two RT-PCR systems for detection (mono-specific) and typing (duplex) of RSV in clinical samples have been developed and validated. The two assays were exploited for studying the dissemination of RSV infection in Riyadh, Saudi Arabia during the winter season 2007/2008. Furthermore, the effect of certain risk factors, including gender, age and disease form, on extend and impact of the infection was analyzed. Of two hundreds collected samples, 70 (35%) were positive for RSV infection, among which 40 (57.1%) were type A and 30 (42.9%) were type B viruses. This investigation is the first that describes the existence of both RSV subtypes in co-ordinance in Saudi Arabia children, with slight dominance of type A viruses. By analyzing the effect of risk factors on the disease epidemiology we confirmed that males, patients at young age and asthmatics are more susceptible for RSV infection and disease progression.
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