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Articles by A. Kousha
Total Records ( 6 ) for A. Kousha
  The aims of this study was to assess the effect of two lactic acid bacteria (LAB), Lactobacillus curvatus and Leuconostoc mesenteroides, originally isolated from gastrointestinal (GI) tract of beluga (Huso huso) and Persian sturgeon (Acipenser persicus), respectively, on growth, survival and digestive enzyme (amylase, lipase and protease) activities and the population level of LAB in the GI tract. The treatments included 10 different groups; control, separate supplements of Lcurvatus and Leumesenteroides at three different counts [2 x 109, 5 x 109 and 9 x 109 colony forming units (CFU) per gram food] and three combinations of the two LAB (2 x 109 + 2 x 109, 5 x 109 + 5 x 109 and 9 x 109 + 9 x 109 CFU per gram food). The bacteria used in this study were added in lyophilized form to chopped Chironomidae. In the beluga study, highest specific growth rate, survival and improved intestinal enzyme activities were noted in the rearing group fed 9 x 109 L. curvatus per gram food. In Persian sturgeon, the inclusion level of 2 x 109 Leu. mesenteroides had similar positive effect. The ability of LAB to colonize the digestive tract seems to involve host specificity, and our bacteriological results are relevant to initiate future probiotic studies in sturgeons and future directions will be discussed.
  A. Kousha and M. Bayat
  The herb Persian hogweed (Heracleum persicum Desf. Ex Fischer) has been used in folkloric and traditional medicine especially in Asia and Middle East to increasing immunity and resistance to some diseases. The aim of the present study was screening antibacterial and antifungal activity of the methanolic extract of mixed leaves and flowers of Heracleum persicum collected from Mazandaran province Kelardasht area was conducted by the disc diffusion method. The methanolic extracts of this plant extracts were tested at concentration of 15 mg per disc and showed antimicrobial impact against selected pathogenic bacteria tested such as Aeromonas salmonicida, Aeromonas hydrophila, Streptococcus iniae (zoonotic agent), Bacillus cereus, Bacillus subtilis Micrococcus luteus, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia, Klebsiella oxytoca and Escherichia coli and fungi such Aspergillus niger and Candida albicans. The result showed that, leaves and flowers extracts of H. persicum have antimicrobial activity over 4 Gram-negative, 4 Gram-positive bacteria and antifungal effect over 2-fungi’s, upon increasing of concentrations of extracts the antimicrobial activities in all of microorganisms also increases. Fungi are more sensitive than bacteria. This species has a huge distribution around the world especially in Europe and United state, achieve to this question that whether those herbs have similar effects at different climate, merits further investigation.
  F. Askarian , A. Matinfar , A. Kousha , M. Bahmani , K. Khorshidi , A. Shenavar and E. Ringo
  The composition of lactic acid bacteria (LAB) in intestine of two species of sturgeon, beluga (Huso huso) and Persian sturgeon (Acipenser persicus), was analyzed. LAB in the gastrointestinal (GI) tract of the two sturgeon species was not similar as LAB population levels in beluga was significantly higher than Persian sturgeon. Six strains of lactic acid bacteria isolated from the GI tract of beluga and Persian sturgeon were characterised by 16S rDNA. Two species of LAB including Enterococcus seriolicida and Leuconostoc mesenteroides were isolated from GI tract of Persian sturgeon and the predominant species was L. mesenteroide. Furthermore, Lactobacillus curvatus, Lactococcus raffinolactis, Lactococcus lactis and Streptococcus sp. were isolated from the GI tract of beluga and the counts of L. curvatus was significantly higher in the GI tract of beluga than other species.
  T. Faghani , A. Kousha , G.H. Azari Takami and S. Faghani
  The aim of this study was the evaluation of the immunostimulatory effects of alginic acid and anti-streptococcus vaccine on the Growth Rate (GR), Specific Growth Rate (SGR), Feed Conversion Ratio (FCR), Condition Factor (KF) and Survival Rate (SR%). The weight range of fish were 5-8 g and the trials were divided to 4 treatments with triplicate groups including control, vaccine, vaccine+alginic acid and alginic acid and each treatment included 3000 fishes. The period of this study was 4 month and biometry was done 6 times (every 20 days intervals) at the end of experimental period the blood samples were taken from all treatments and hematological parameters were evaluated. The results showed that GR had a significant increase in vaccine+alginic acid treatment when compared with the control group (p<0.05). SR% showed a significant increase in vaccine+alginic acid and alginic acid treatment in compared with control group (p<0.05). Significant elevation in the percentage of lymphocyte observed in vaccine, vaccine+alginic acid and alginic acid treatment when compared with control group have observed. According to the results, it seems that prescription of vaccine and alginic acid were stimulated lymphocyte proliferation in all treated groups and therefore fish resistance increase to environmental stress and pathogen.
  F. Askarian and A. Kousha
  In this study, the effect of 90 days maintenance-feeding period on the acute stress response of 45 one year old beluga, Huso huso, to handling and confinement were determined. The fishes were divided to three treatments including Control, High food ration (H>L) and low food ration (L>H). Fishes were raised on an optimal feeding level of 4% of body weight per day during the experiment. The fishes in High treatment (H>L) was fed 4% of body weight per day for 60 days and food ration was reduced to 1% of body weight per days in the last month of experiment. The fishes in Low treatment (L>H) was fed 1% of body weight per day for 60 days and food ration was suddenly increased to 4% of body weight per days in the last month of experiment. All fishes were sampled at the end of experiment one time before exposure to stress and 3 times including half an hour, 1 and 3 h after stress. The results revealed that food ration had significant effects on the growth rate and food conversion values because fishes fed the high ration performing better than those on a lower level. Prior to the application of the stressor, only plasma levels of triglycerides were lower in fish fed a low food ration at the last month of experiment (H>L). Feeding history Influenced the onset of the stress response with stressor-induced elevations of plasma cortisol, glucose and free fatty acids being higher in fish fed a high ration compared with those fed a low ration prior to sampling (H>L). These results suggest that feeding history through modification of the energy reserves can influence the onset of the acute stress response.
  F. Askarian and A. Kousha
  Data on the concentrations of some blood constituents of reared Beluga sturgeon, Huso huso, including Serum cortisol, glucose, triglyceride, cholesterol, osmolality, Na+, K+, Ca2+, ALP that reared under different light regimes were measured. The light regimes were consist of natural photoperiod (NP), continuous dark (0L:24D), continuous light (24L:0D) and long day regime (16L:8D) and tested on 4 group of 30 one year old reared Beluga for 6 month which sampled four times during the experiment. No significant difference in serum cortisol and ALP levels was found between treatments (p>0.05). Elevations of serum cortisol, glucose, cholestrol and triglyceride concentrations were reported in continues dark regime. Increase of serum osmolality, Na+, K+ and decrease of Ca2+ were also associated with increase of cortisol levels. Results are compared with the few data available in condroestean fish and with those on teleosts.
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