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| Articles
by
A. Keyvan |
Total Records (
3 ) for
A. Keyvan |
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A. Keyvan
,
S. Moini
,
N. Ghaemi
,
A.A. Haghdoost
,
S. Jalili
and
M. Pourkabir
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The study was designed to investigate the effect of
duration of frozen storage on chemical analysis, lipid damage and extractability
of Myofibrillar proteins of Kutum (Rutilus frisi kutum. The fish
were collected from Anzaly landings in north of Iran and were subjected
to 12 months of frozen storage and analyzed at intervals of three month.
Protein content ranges from 21.8±0.01 to 19.9±0.01. Protein
decrease with increasing duration of frozen storage; fish samples that
were for frozen thirty days; having highest protein content 21.8±0.01
while the least 19.9±0.01 was recorded for fish samples that were
frozen for 12 months. Similar results obtained for the fat content where
the highest fat content 3.21±0.01 was recorded for the fish samples
that were for frozen 30 days and the least value was recorded for those
stored for 12 months. The least moisture content was observed for fish
samples the was stored for 12 months but, the highest Ash content was
observed for fish samples the was stored for 12 month. Lipid damage were
measured on the basis of Free Fatty Acids (FFA), Peroxide Value (PV),
Thiobarbituric acid index (TBA-i). PV, TBARS and FFA concentration of
frozen Caspian Sea white fish stored at -18°C; the temporal variation
of these three variables were statistically significant (p<0.001).
SDS-PAGE patterns showed that myosin heavy chain was much more susceptible
to hydrolysis than actin. |
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M. Norouzi
,
M. Pourkazemi
,
A. Keyvan
,
S.M.R. Fatemi
and
B. Kazemi
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In this study, 197 samples of adult stellate sturgeon from four fishery regions were collected. DNA was extracted using 15 pairs of microsatellite primers, Polymerase Chain Reaction (PCR) was conducted. DNA bands were analyzed using Biocapt and GenAlex software package. Out of 15 microsatellite primers, 11 loci were produced, in which 10 of them were polymorphic and 1 monomorph. Analyses revealed that average of 13 alleles per locus (range 8 to 18 alleles per locus). Average observed and expected heterozygosity were 0.650 and 0.855 and significant genetic differences between 4 regions were observed (p≤0.01). Deviations from Hardy-Weinberg equilibrium were in most cases. Maximum genetic difference were observed between regions 2 and 4 (FST = 0.063, Nm = 3.728). These results indicate that at least, 3 populations of stellate sturgeon exist in the South Caspian Sea. Population of stellate sturgeon in region 2 where Sefidrud drainage is located was consider as independent population, therefore management of this unique stocks for restocking and conservation of gene pools is highly recommended. |
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M.F. Lasheidani
,
S.N. Balouchi
,
A. Keyvan
,
S. Jamili
and
K. Falakrou
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Morphological assessment of sexually mature Rutilus
frisii kutum Kamenskii 1901 caught from the rivers (Shirud, Khoshkrud,
Sepidrud and Chelavand Rivers) flowing in the southwest Caspian Sea region
was conducted and sperm volume, total sperm count and sperm concentration
of abnormal sperms were determined after exposing the spawners to 60%
herbicide butachlor (machete). Spawners under study were maintained in
tanks (1000 L) at the Shahid Ansari Teleost Fish Hatchery and exposed
to two different concentrations (25 and 75% of its LC50 value)
of butachlor. Results obtained indicate that exposure to high butachlor
toxicity (75% of its LC50 value) decreased sperm volume to
0.61 ± 0.42 cc in 2-3 year old fishes and to 0.55 ± 0.42
cc in fishes above 3 years of age, while that in fish exposed to low butachlor
toxicity (25% of its LC50 value) decreased to 1.55 ±
0.42 cc in 2-3 year old fishes and to 1.28 ± 0.42 cc in fishes
above 3 years of age. The sperm volume under normal conditions in R.
frisii kutum is 4.6 ± 0.42 cc in 2-3 years old and 4.58 ±
0.42 cc in fishes above 3 years of age. The total sperm count in R.
frisii kutum is 39.74 ± 2.5 billion spermatozoa cc-1
in 2-3 year olds and 42.99 ± 2.5 billion spermatozoa cc-1
in fishes above 3 years of age. When exposed to high butachlor toxicity,
total sperm count dropped to 16.92 ± 2.5 billion spermatozoa cc-1
in 2-3 year olds and to 15.98 ± 2.5 billion spermatozoa cc-1
in fishes above 3 years of age. Similarly total sperm count in R.
frisii kutum exposed to low butachlor toxicity was recorded as 23.6
± 2.5 billion spermatozoa cc-1 in 2-3 year olds and
29.4 ± 2.5 billion spermatozoa cc-1 in fishes above
3 years of age. Under normal conditions, on the basis of morphology, spermatozoa
showed only 10 ± 1.92% of abnormal sperms. The number of abnormal
sperms increased by 28.6 ± 1.92% in fishes exposed to high butachlor
toxicity, while that in fishes exposed to low butachlor toxicity increased
by 19.7 ± 1.92% in 2-3 year olds and 16.6 ± 19.2% in fishes
above 3 years of age. It is evident from the results obtained that increase
in level of pollution caused a decrease in sperm volume but an increase
in the percentage of abnormal sperms. |
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