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Articles by A. Javanmard
Total Records ( 6 ) for A. Javanmard
  A. Mohsenpour Azary , F. Mohebbi , A. Eimanifar , A. Javanmard and Agamaliyev F.Q. Aliyev
  Problem statement: Since there is no evidence to identify present species in the Bukan dam reservoir, therefore, this study was conducted to provide background for fisheries purposes. Approach: The abundance and species composition of ciliates were analyzed in the Bukan Dam reservoir (west Azerbaijan, Iran) from January to December 2007. Surface water samples were collected at monthly intervals at two different points, open water (Station 1, 3) and in the shallowest area of the reservoir (Station 2, 4) and examined for planktonic protozoa composition and density. Chlorophyll a was analyzed and a few ecological parameters such as dissolved oxygen, pH, turbidity and temperature were measured. Results: Totally 50 ciliates species were found in Bukan dam reservoir. Concerning the protozoa density, a high-density period was detected from January to April with a mean number of 2.86x103 cells.L-1 protozoa at Stations 1, 3 and 4.16x103 cells.L-1 protozoa at shallowest areas at stations 2, 4 thanks to disappear of phytoplankton in the winter. The summer development of protozoa was possible thanks to the development of bacteria and moderate metazooplankton densities due to the appearance of non-edible algae. The density of ciliates was low during the rest of the year. The mean number of organisms at the high-density period was approximately 4-fold more than that obtained during the low-density one. The ciliates occurring at the highest densities were Coleps tessellates, Paradileptus elephantinus, Zosterograptus labiatus, Cyclidium citrullus. Conclusion/Recommendations: It was concluded that ciliates density have important role for saprobic condition of Bukan reservoir. Therefore, it can be recommended to determine more effective parameters for density of ciliates and management policies must be programmed in order to improve ecological condition for this reservoir.
  M.R. Nassiry , A. Javanmard and Reza Tohidi
  Problem statement: A wide range of studies for the assessment of genetic diversity in livestock breed were conducted using genetic distance. For high-accuracy and unbiased estimation sampling methods, criteria of choosing type of DNA markers, distance measurement strategies, cluster analysis will be important for any genetic diversity projects. Approach: Main objective of this short review is focusing on application statistical procedures and methods in analysis of genetic diversity data in animals. Results: There is no simple strategy to address for best and effectively genetic diversity results by the way regarding to some important factors can make reliable results for next analysis. Conclusion: There is still a distinct need for developing comprehensive and user-friendly statistical packages that facilitate an integrated analysis of different data sets for generating reliable information about genetic relationships, genome diversity, and favorable allele variation. Equally important and perhaps more challenging, is the concerted and planned utilization of genome information in animal breeding programs on the basis of knowledge accrued from studies on genetic diversity.
  G. Elyasi , J. Shodja , M.R. Nassiry , A. Tahmasebi , O. Pirahary and A. Javanmard
  β-lactoglobulin is the major milk whey protein in ruminants and its coding gene located on ovine chromosome 3. This protein produces in mammary glands during pregnancy and lactation stages. Studies have shown that the protein is polymorphic in many breeds of sheep. This is the result of single base pair substitution in β-lactoglobulin gene that also rises to RsaI restriction Fragment Length Polymorphism (RFLP). Blood samples were supplied from 142 animals of Iranian sheep breeds (Ghezel, Afshary, Moghani, Makoii and Arkharmerino). Genomic DNA was extracted from 100 μL blood sample according to Boom method modified by Shaikhayev. Gel monitoring and spectrophotometeric methods were used for determination of DNA quality and quantity. BLg 5 and 3 primers amplified a 452 bp fragment from exon II of ovine β-lactoglobulin gene. Products of amplification were recognized by electrophoresis on 1% agarose gel stained with ethidium bromide. RsaI enzyme was used for restriction of PCR products. Digestion products were separated by electrophoresis on 8% non-denaturant polyacrylamide gel and visualized after staining with ethidium bromide on UV gel documentation. PopGen 32 software (ver. 1.31) was used to estimating the frequency of allele, genotype and Hardy-Weinberg equilibrium. Frequency of A-allele in Ghezel, Afshary, Moghani, Makoii and Arkharmerino sheep was 56, 34, 36, 53 and 48%, respectively. The populations were in Hardy-Weinberg equilibrium except of Afshary breed.
  A. Javanmard , K. Khaledi , N. Asadzadeh and A.R. Solimanifarjam
  The objective of this study was to assess the association of polymorphism in leptin gene with breeding value of milk traits in Iranian Holstein cattle. Traits analyzed were breeding values of Milk and Fat production. A strategy employing polymerase chain reaction was used to amplify a 422 bp from blood, semen. Digestion of polymerase chain reaction products with Sau3AI revealed two alleles: Allele A was 390, 32 fragment and allele B was 303, 88 and 32 (only 303 fragment visible on the gel). Three patterns were observed frequencies were 0.900, 0.100 and 0.00 for AA, AB and BB, respectively (p≤0.21). The AB bulls were superior for milk fat production, in relation to homozygous AA. The Bovine leptin gene is strong candidates in the dairy cattle QTL search. This polymorphism could be further evaluated for marker assisted selection and the developed PCR methodology would expedite screening for large numbers of animal required for such studies.
  N. Asadzadeh , A. Javanmard and M.R. Nassiry
  Clean, high molecular weight DNA is pre-requisite for DNA markers. The amount and quality of DNA is a crucial point for all further analysis. A unique advantage of these PCR techniques is the rapid DNA analysis of many animal samples using small quantities of DNA. Thus, a simple and rapid DNA extraction method is needed for studies such as genetic analysis that require large populations. Several methods for minimizing the DNA extraction steps have been reported but they require a large amount of animal tissues. In addition, bleeding and management of sampling and storage of the blood sample in freezers is often difficult due to space constraints. To overcome these problems, some techniques developed a DNA extraction method using the milk or hair root or semen. Researchers compared 4 methods of rapid DNA extraction with isolations of mammalian whole blood samples. DNA extraction methods included boiling, salting out, phenol-chloroform and silica gel procedures. Spectrophotometry and gel monitoring evaluated the DNA yield and purity for the 4 methods. The silica gel and phenol-chloroform methods yielded significantly purity and higher concentration of extracted DNA compared with other DNA extraction methods.
  M. Ramin , A.R. Alimon , J.M. Panandam , K. Sijam , A. Javanmard and N. Abdullah
  The digestion and Volatile Fatty Acid (VFA) production from rice straw and oil palm fronds by cellulolytic bacteria isolated from the termite Coptotermes curvignathus were investigated. The bacteria were Acinetobacter strain Raminalimon, Enterobacter aerogenes strain Razmin C, Enterobacter cloacae strain Razmin B, Bacillus cereus strain Razmin A and Chryseobacterium kwangyangense strain Cb. Acinetobacter strain Raminalimon is an aerobic bacterium, while the other species are facultative anaerobes. There were significant differences (p<0.05) among the bacteria for Dry Matter (DM) lost and acetic acid production from rice straw and Acinetobacter strain Raminalimon showed the highest activity. The facultative bacteria C. kwangyangense strain Cb (cfu mL-1 231x10-6, OD: 0.5), E. cloacae (cfu mL-1 68x10-7, OD: 0.5) and E. aerogenes (cfu mL-1 33x10-7, OD: 0.5) were used for digestion study with the rumen fluid microflora. The in vitro gas production technique was applied for the comparative study and the parameters measured were pH, gas (volume), dry matter lost, acetic acid, propionic acid and butyric acid concentrations. pH was not significantly (p<0.05) different among the five treatments. The bacterium C. kwangyangense strain Cb showed the highest activity (p<0.05) for DM lost, acetic acid, propionic acid and butyric acid production from rice straw when compared to the other bacterial activities. There was no significance (p<0.05) difference between the three bacteria for the dry matter lost of oil palm fronds but the production of Volatile Fatty Acids (VFA) was significantly (p<0.05) high in the treatment which was inoculated with C. kwangyangense strain Cb. The Gen Bank NCBI/EMBL accession numbers for the bacterial strains are EU332791, EU305608, EU305609, EU294508 and EU169201.
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